Cardiolipin synthase of Arabidopsis thaliana
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Abstract
Functional expression studies in microorganisms showed that the Arabidopsis thaliana gene At4g04870 represents the cardiolipin synthase ( CLS) gene encoding a hydrophobic preprotein of 38 kDa with a cleavable signal peptide for the import into mitochondria. CLS of Arabidopsis over-expressed in Escherichia coli has an alkaline pH optimum, a strict requirement for divalent cations and a distinctly lower K m for cytidinediphosphate-diacylglycerol than for phosphatidylglycerol. It displayed a preference for both its substrates esterified with unsaturated acyl groups. Solubilization and purification experiments revealed that the protein requires a defined phospholipid environment, particularly the presence of cardiolipin, to acquire its catalytically active conformation.
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Publication:
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FEBS Letters
Pub Date:
- January 2005 DOI:
- Bibcode:
- 2005FEBSL.579.2161N Keywords:
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- CDP-DAG;
- cytidinediphosphate-diacylglycerol;
- CL;
- cardiolipin;
- CLS;
- cardiolipin synthase;
- PC;
- phosphatidylcholine;
- PE;
- phosphatidylethanolamine;
- PG;
- phosphatidylglycerol;
- PGP;
- phosphatidylglycerophosphate;
- PI;
- phosphatidylinositol;
- Cytidinediphosphate-diacylglycerol;
- Cardiolipin synthase;
- Functional expression;
- Mitochondria;
- Phospholipid synthesis;
- Arabidopsis thaliana