GMD - Corticosterone (2MEOX) (2TMS) MP - InChI=1S/C29H52N2O4Si2/c1-28-16-15-21(30-32-3)17-20(28)11-12-22-23-13-14-24(25(31-33-4)19-34-36(5,6)7)29(23,2)18-26(27(22)28)35-37(8,9)10/h17,22-24,26-27H,11-16,18-19H2,1-10H3/b30-21+,31-25+/t22-,23-,24+,26-,27+,28-,29-/m0/s1
- ️hummel@mpimp-golm.mpg.de
- ️Tue Aug 31 2021
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| attribute | text | details |
|---|---|---|
| deconvolution | ChromaTOF | OFFSET: "just above noise"; SMOOTHING: 20; PEAK WIDTH: 6; S/N: 2 |
| derivatization | MEOX; TMS | METHOXYAMINATION: 90min at 30°C; TRIMETHYLSILYLATION: 30min at 37°C |
| detector | MS-TOF | m/z = 70-600; scans:20/s; Pegasus III TOF mass spectrometer |
| extraction | water:chloroform | (2:1; v/v) 15min at 70°C |
| gas chromatograph | Agilent GC 6890 | |
| ion source | EI | 70eV |
| mass spectrometry instrument | Pegasus III TOF mass spectrometer | |
| metabolic inactivation | shock-frozen in liquid nitrogen | |
| RI | ALKANE | C10, C12, C15, C18, C19, C22, C28, C32, C36 |
| separation | GC | COLUMN:5%phenyl-95%dimethylpolysiloxane, 30+10m, ID:0.25mm, DF:0.25µm, 5PDM VF-5ms (Varian, Darmstadt, Germany); PROGRAM:iso1min 70°C, ramp 9°C/min, iso 5min 350°C; FLOW:Helium, 0.6mL/min; INJECTION:1µL, splitless, 230°C; TRANSFER:250°C; IONSOURCE:250°C |