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Cucumis sativus fruit-potential antioxidant, anti-hyaluronidase, and anti-elastase agent - Archives of Dermatological Research

  • ️Mukherjee, Pulok K.
  • ️Tue Dec 14 2010

Abstract

The aim of this study was to screen the antioxidant, anti-hyaluronidase, and anti-elastase activity of the lyophilized juice of Cucumis sativus fruit (CSLJ). The CSLJ was subjected to DPPH and superoxide radical scavenging assay in reference to butylated hydroxytoluene. The hyaluronidase and elastase inhibitory assay was performed in reference to oleanolic acid. Furthermore, the activities have been rationalized with HPLC analysis of the CSLJ with standard reference compound of ascorbic acid. The CSLJ exhibited DPPH-free radical and superoxide radical scavenging activity, IC50 at a concentration of 14.73 ± 1.42 and 35.29 ± 1.30 μg/mL, respectively. The CSLJ also showed strong anti-hyaluronidase (c P < 0.001) and anti-elastase (c P < 0.001) activity, IC50 at a concentration of 20.98 ± 1.78 and 6.14 ± 1.74 μg/mL, respectively. The HPLC ‘chromatogram’ of standard and CSLJ showed specific peak at retention time 2.905 and 3.066 min, respectively. Content of ascorbic acid was calculated with respect to the standard compound and it was found to be 3.5 ± 0.23% w/w. CSLJ is the rich source of ascorbic acid and this study thereby rationalizes the use of C. sativus as potential anti-wrinkle agent in cosmetic products.

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Acknowledgments

The study was supported by Department of Science and Technology, through Drug and Pharmaceutical Research Programme [DST-DPRP, File No. VI-D&P/287/08-09/TDT], Government of India, New Delhi and Parker Robinson Pvt. Ltd., Kolkata, India.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. School of Natural Product Studies, Department of Pharmaceutical Technology, Jadavpur University, Kolkata, 700 032, India

    Neelesh K. Nema, Niladri Maity & Pulok K. Mukherjee

  2. Parker Robinson (P) Ltd., 1, Nimak Mahal Road, Kolkata, 700043, India

    B. Sarkar

Authors

  1. Neelesh K. Nema

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  2. Niladri Maity

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  3. B. Sarkar

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  4. Pulok K. Mukherjee

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Correspondence to Pulok K. Mukherjee.

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Nema, N.K., Maity, N., Sarkar, B. et al. Cucumis sativus fruit-potential antioxidant, anti-hyaluronidase, and anti-elastase agent. Arch Dermatol Res 303, 247–252 (2011). https://doi.org/10.1007/s00403-010-1103-y

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  • Received: 08 October 2010

  • Revised: 20 November 2010

  • Accepted: 22 November 2010

  • Published: 14 December 2010

  • Issue Date: May 2011

  • DOI: https://doi.org/10.1007/s00403-010-1103-y

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