pubmed.ncbi.nlm.nih.gov

Chromatin techniques for plant cells - PubMed

Chromatin techniques for plant cells

Chris Bowler et al. Plant J. 2004 Sep.

Free article

Abstract

A large number of recent studies have demonstrated that many important aspects of plant development are regulated by heritable changes in gene expression that do not involve changes in DNA sequence. Rather, these regulatory mechanisms involve modifications of chromatin structure that affect the accessibility of target genes to regulatory factors that can control their expression. The central component of chromatin is the nucleosome, containing the highly conserved histone proteins that are known to be subject to a wide range of post-translational modifications, which act as recognition codes for the binding of chromatin-associated factors. In addition to these histone modifications, DNA methylation can also have a dramatic influence on gene expression. To accommodate the burgeoning interest of the plant science community in the epigenetic control of plant development, a series of methods used routinely in our laboratories have been compiled that can facilitate the characterization of putative chromatin-binding factors at the biochemical, molecular and cellular levels.

PubMed Disclaimer

Cited by

Uridylation and the SKI complex orchestrate the Calvin cycle of photosynthesis through RNA surveillance of TKL1 in Arabidopsis.
Wang X, Kong W, Wang Y, Wang J, Zhong L, Lao K, Dong X, Zhang D, Huang H, Mo B, Yu Y, Ren G. Wang X, et al. Proc Natl Acad Sci U S A. 2022 Sep 20;119(38):e2205842119. doi: 10.1073/pnas.2205842119. Epub 2022 Sep 12. Proc Natl Acad Sci U S A. 2022. PMID: 36095196 Free PMC article.
Integration of stress-related and reactive oxygen species-mediated signals by Topoisomerase VI in Arabidopsis thaliana.
Simková K, Moreau F, Pawlak P, Vriet C, Baruah A, Alexandre C, Hennig L, Apel K, Laloi C. Simková K, et al. Proc Natl Acad Sci U S A. 2012 Oct 2;109(40):16360-5. doi: 10.1073/pnas.1202041109. Epub 2012 Sep 17. Proc Natl Acad Sci U S A. 2012. PMID: 22988090 Free PMC article.
Feedback Regulation of ABA Signaling and Biosynthesis by a bZIP Transcription Factor Targets Drought-Resistance-Related Genes.
Zong W, Tang N, Yang J, Peng L, Ma S, Xu Y, Li G, Xiong L. Zong W, et al. Plant Physiol. 2016 Aug;171(4):2810-25. doi: 10.1104/pp.16.00469. Epub 2016 Jun 20. Plant Physiol. 2016. PMID: 27325665 Free PMC article.
Epigenetics and its implications for plant biology. 1. The epigenetic network in plants.
Grant-Downton RT, Dickinson HG. Grant-Downton RT, et al. Ann Bot. 2005 Dec;96(7):1143-64. doi: 10.1093/aob/mci273. Epub 2005 Oct 27. Ann Bot. 2005. PMID: 16254022 Free PMC article. Review.
Chromatin immunoprecipitation (ChIP) of plant transcription factors followed by sequencing (ChIP-SEQ) or hybridization to whole genome arrays (ChIP-CHIP).
Kaufmann K, Muiño JM, Østerås M, Farinelli L, Krajewski P, Angenent GC. Kaufmann K, et al. Nat Protoc. 2010 Mar;5(3):457-72. doi: 10.1038/nprot.2009.244. Epub 2010 Feb 18. Nat Protoc. 2010. PMID: 20203663

Chromatin techniques for plant cells - PubMed