Stimulus-triggered fate conversion of somatic cells into pluripotency - PubMed

Affiliations

• PMID: 24476887
• DOI: 10.1038/nature12968

Stimulus-triggered fate conversion of somatic cells into pluripotency

Haruko Obokata et al. Nature. 2014.

Retraction in

• Retraction: Stimulus-triggered fate conversion of somatic cells into pluripotency.

  Obokata H, Wakayama T, Sasai Y, Kojima K, Vacanti MP, Niwa H, Yamato M, Vacanti CA. Obokata H, et al. Nature. 2014 Jul 3;511(7507):112. doi: 10.1038/nature13598. Nature. 2014. PMID: 24990753 No abstract available.

Abstract

Here we report a unique cellular reprogramming phenomenon, called stimulus-triggered acquisition of pluripotency (STAP), which requires neither nuclear transfer nor the introduction of transcription factors. In STAP, strong external stimuli such as a transient low-pH stressor reprogrammed mammalian somatic cells, resulting in the generation of pluripotent cells. Through real-time imaging of STAP cells derived from purified lymphocytes, as well as gene rearrangement analysis, we found that committed somatic cells give rise to STAP cells by reprogramming rather than selection. STAP cells showed a substantial decrease in DNA methylation in the regulatory regions of pluripotency marker genes. Blastocyst injection showed that STAP cells efficiently contribute to chimaeric embryos and to offspring via germline transmission. We also demonstrate the derivation of robustly expandable pluripotent cell lines from STAP cells. Thus, our findings indicate that epigenetic fate determination of mammalian cells can be markedly converted in a context-dependent manner by strong environmental cues.

Comment in

• Cell biology: Potency unchained.

  Smith A. Smith A. Nature. 2014 Jan 30;505(7485):622-3. doi: 10.1038/505622a. Nature. 2014. PMID: 24476883 Retracted. No abstract available.

• Stress management: a new path to pluripotency.

  Pera MF. Pera MF. Cell Stem Cell. 2014 Mar 6;14(3):273-4. doi: 10.1016/j.stem.2014.02.009. Cell Stem Cell. 2014. PMID: 24607402

• Stimulus-triggered acquisition of pluripotency: revolutionizing human disease modeling and regenerative therapies?

  Seibler P, Klein C. Seibler P, et al. Mov Disord. 2014 Apr;29(4):451. doi: 10.1002/mds.25879. Mov Disord. 2014. PMID: 24700468 No abstract available.

• STAP cells are derived from ES cells.

  Konno D, Kasukawa T, Hashimoto K, Itoh T, Suetsugu T, Miura I, Wakana S, Carninci P, Matsuzaki F. Konno D, et al. Nature. 2015 Sep 24;525(7570):E4-5. doi: 10.1038/nature15366. Nature. 2015. PMID: 26399834 No abstract available.

• Failure to replicate the STAP cell phenomenon.

  De Los Angeles A, Ferrari F, Fujiwara Y, Mathieu R, Lee S, Lee S, Tu HC, Ross S, Chou S, Nguyen M, Wu Z, Theunissen TW, Powell BE, Imsoonthornruksa S, Chen J, Borkent M, Krupalnik V, Lujan E, Wernig M, Hanna JH, Hochedlinger K, Pei D, Jaenisch R, Deng H, Orkin SH, Park PJ, Daley GQ. De Los Angeles A, et al. Nature. 2015 Sep 24;525(7570):E6-9. doi: 10.1038/nature15513. Nature. 2015. PMID: 26399835 No abstract available.

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