Reconstitution and structure of a plant NLR resistosome conferring immunity - PubMed

Affiliations

• PMID: 30948527
• DOI: 10.1126/science.aav5870

Reconstitution and structure of a plant NLR resistosome conferring immunity

Jizong Wang et al. Science. 2019.

Abstract

Nucleotide-binding, leucine-rich repeat receptors (NLRs) perceive pathogen effectors to trigger plant immunity. Biochemical mechanisms underlying plant NLR activation have until now remained poorly understood. We reconstituted an active complex containing the Arabidopsis coiled-coil NLR ZAR1, the pseudokinase RKS1, uridylated protein kinase PBL2, and 2'-deoxyadenosine 5'-triphosphate (dATP), demonstrating the oligomerization of the complex during immune activation. The cryo-electron microscopy structure reveals a wheel-like pentameric ZAR1 resistosome. Besides the nucleotide-binding domain, the coiled-coil domain of ZAR1 also contributes to resistosome pentamerization by forming an α-helical barrel that interacts with the leucine-rich repeat and winged-helix domains. Structural remodeling and fold switching during activation release the very N-terminal amphipathic α helix of ZAR1 to form a funnel-shaped structure that is required for the plasma membrane association, cell death triggering, and disease resistance, offering clues to the biochemical function of a plant resistosome.

Comment in

• A resistosome-activated 'death switch'.

  Adachi H, Kamoun S, Maqbool A. Adachi H, et al. Nat Plants. 2019 May;5(5):457-458. doi: 10.1038/s41477-019-0425-9. Nat Plants. 2019. PMID: 31036914 No abstract available.

• The Rise of Plant Resistosomes.

  Mermigka G, Sarris PF. Mermigka G, et al. Trends Immunol. 2019 Aug;40(8):670-673. doi: 10.1016/j.it.2019.05.008. Epub 2019 Jun 7. Trends Immunol. 2019. PMID: 31182341

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