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The Clinical Proteomic Technologies for Cancer | Details for CPTC-ODC1-1

  • ️Thu Sep 09 2021

Ornithine Decarboxylase 1

Background

Catalog Number:

CPTC-ODC1-1

RRID:

AB_1553747

Target Antigen:

Ornithine Decarboxylase 1

Isotype:

IgG1

Species:

Mouse Monoclonal Antibody

Last Updated:

09/09/2021

Antigen Recognition(s):

Recombinant Full-length, Endogenous

SOP:

Purchase
External Links
Characterization Data [Compare Characterization Data]
  Immunofluorescence - HPA

Click to enlarge image Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is partly supported by literature or no literature is available. Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol. Human assay: A-431 fixed with PFA, dilution: 1:30 Human assay: U-2 OS fixed with PFA, dilution: 1:30 Human assay: U-251 MG fixed with PFA, dilution: 1:30 Click image to enlarge

Result: Positive

Results provided by the Human Protein Atlas (www.proteinatlas.org). The subcellular location is partly supported by literature or no literature is available. Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.
Human assay: A-431 fixed with PFA, dilution: 1:30
Human assay: U-2 OS fixed with PFA, dilution: 1:30
Human assay: U-251 MG fixed with PFA, dilution: 1:30


  Western Blot - Recombinant Protein or Peptide

Click to enlarge image Western Blot Using CPTC-ODC1-1 as primary Ab against Ag 10532(Lane 2). Also included are Molecular Weight markers (Lane 1) and mouse IgG positive control (Lane 3). Click image to enlarge

Result: Positive

Western Blot Using CPTC-ODC1-1 as primary Ab against Ag 10532(Lane 2). Also included are Molecular Weight markers (Lane 1) and mouse IgG positive control (Lane 3).


Background

Catalog Number:

CPTC-ODC1-2

RRID:

AB_2267589

Target Antigen:

Ornithine Decarboxylase 1

Isotype:

IgG2a

Species:

Mouse Monoclonal Antibody

Last Updated:

05/28/2024

Antigen Recognition(s):

Recombinant Full-length, Endogenous

SOP:

Purchase
Characterization Data [Compare Characterization Data]
  CyTOF

Click to enlarge image Imaging mass cytometry on bone marrow core using CPTC-ODC1-2 metal-labeled antibody. Data shows an overlay of the target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (liver, bone marrow, spleen, colon, pancreas, breast, lung, endometrium, and kidney) and cancer tissues (breast, colon, ovarian, and lung). Click image to enlarge

Result: Positive

Imaging mass cytometry on bone marrow core using CPTC-ODC1-2 metal-labeled antibody. Data shows an overlay of the target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (liver, bone marrow, spleen, colon, pancreas, breast, lung, endometrium, and kidney) and cancer tissues (breast, colon, ovarian, and lung).


  NCI 60 Protein Array

Click to enlarge image Protein Array in which CPTC-ODC1-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red). Click image to enlarge

Result: Positive

Protein Array in which CPTC-ODC1-2 is screened against the NCI60 cell line panel for expression. Data is normalized to a mean signal of 1.0 and standard deviation of 0.5. Color conveys over-expression level (green), basal level (blue), under-expression level (red).


  Western Blot - Recombinant Protein or Peptide

Click to enlarge image Western Blot Using CPTC-ODC1-2 as primary Ab against Ag 10532(Lane 2). Also included are Molecular Weight markers (Lane 1) and mouse IgG positive control (Lane 3). Click image to enlarge

Result: Positive

Western Blot Using CPTC-ODC1-2 as primary Ab against Ag 10532(Lane 2). Also included are Molecular Weight markers (Lane 1) and mouse IgG positive control (Lane 3).


Background

Catalog Number:

CPTC-ODC1-3

RRID:

AB_1553749

Target Antigen:

Ornithine Decarboxylase 1

Isotype:

IgG2a

Species:

Mouse Monoclonal Antibody

Last Updated:

05/28/2024

Antigen Recognition(s):

Recombinant Full-length, Endogenous

SOP:

Purchase
Characterization Data [Compare Characterization Data]
  CyTOF

Click to enlarge image Imaging mass cytometry on breast cancer tissue core using CPTC-ODC1-3 metal-labeled antibody. Data shows an overlay of the target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (liver, bone marrow, spleen, placenta, prostate, colon, pancreas, breast, lung, endometrium, appendix, and kidney) and cancer tissues (breast, colon, ovarian, lung, and prostate). Click image to enlarge

Result: Positive

Imaging mass cytometry on breast cancer tissue core using CPTC-ODC1-3 metal-labeled antibody. Data shows an overlay of the target protein signal (red) and DNA (blue). Dilution: 1:100 of 0.5mg/mL stock. Signal was also obtained in other normal tissues (liver, bone marrow, spleen, placenta, prostate, colon, pancreas, breast, lung, endometrium, appendix, and kidney) and cancer tissues (breast, colon, ovarian, lung, and prostate).


  Western Blot - Recombinant Protein or Peptide

Click to enlarge image Western Blot Using CPTC-ODC1-3 as primary Ab against Ag 10532(Lane 2). Also included are Molecular Weight markers (Lane 1) and mouse IgG positive control (Lane 3). Click image to enlarge

Result: Positive

Western Blot Using CPTC-ODC1-3 as primary Ab against Ag 10532(Lane 2). Also included are Molecular Weight markers (Lane 1) and mouse IgG positive control (Lane 3).


Background

NCI Identification Number:

10532

Antigen Name:

Ornithine Decarboxylase 1

CPTC Name:

CPTC-ODC1

Aliases:

ODC1; EC 4.1.1.17; ODC

Function:

This gene encodes the rate-limiting enzyme of the polyamine biosynthesis pathway which catalyzes ornithine to putrescine. The activity level for the enzyme varies in response to growth-promoting stimuli and exhibits a high turnover rate in comparison to other mammalian proteins. Originally localized to both chromosomes 2 and 7, the gene encoding this enzyme has been determined to be located on 2p25, with a pseudogene located on 7q31-qter.

Chromosomal Localization:

2p25

Expression System:

E. Coli

Accession Number:

BC025296

UniProt Accession Number:

P11926

DNA Source:

HIP:HsCD00005772

Immunogen:

Recombinant Full Length Protein

Vector Name:

pMCSG7

Extinction Coefficient:

53205

Buffers:

50mM NH4HC03

Expressed Sequence:

SNAMNNFGNEEFDCHFLDEGFTAKDILDQKINEVSSSDDKDAFYVADLGD
ILKKHLRWLKALPRVTPFYAVKCNDSKAIVKTLAATGTGFDCASKTEIQL
VQSLGVPPERIIYANPCKQVSQIKYAANNGVQMMTFDSEVELMKVARAHP
KAKLVLRIATDDSKAVCRLSVKFGATLRTSRLLLERAKELNIDVVGVSFH
VGSGCTDPETFVQAISDARCVFDMGAEVGFSMYLLDIGGGFPGSEDVKLK
FEEITGVINPALDKYFPSDSGVRIIAEPGRYYVASAFTLAVNIIAKKIVL
KEQTGSDDEDESSEQTFMYYVNDGVYGSFNCILYDHAHVKPLLQKRPKPD
EKYYSSSIWGPTCDGLDRIVERCDLPEMHVGDWMLFENMGAYTVAAASTF
NGFQRPTIYYVMSGPAWQLMQQFQNPDFPPEVEEQDASTLPVSCAWESGM
KRHRAACASASINV

Native Sequence:

MNNFGNEEFDCHFLDEGFTAKDILDQKINEVSSSDDKDAFYVADLGDILK
KHLRWLKALPRVTPFYAVKCNDSKAIVKTLAATGTGFDCASKTEIQLVQS
LGVPPERIIYANPCKQVSQIKYAANNGVQMMTFDSEVELMKVARAHPKAK
LVLRIATDDSKAVCRLSVKFGATLRTSRLLLERAKELNIDVVGVSFHVGS
GCTDPETFVQAISDARCVFDMGAEVGFSMYLLDIGGGFPGSEDVKLKFEE
ITGVINPALDKYFPSDSGVRIIAEPGRYYVASAFTLAVNIIAKKIVLKEQ
TGSDDEDESSEQTFMYYVNDGVYGSFNCILYDHAHVKPLLQKRPKPDEKY
YSSSIWGPTCDGLDRIVERCDLPEMHVGDWMLFENMGAYTVAAASTFNGF
QRPTIYYVMSGPAWQLMQQFQNPDFPPEVEEQDASTLPVSCAWESGMKRH
RAACASASINV

Calculated Isoelectric Point:

5.1

Molecular Weight:

51420

Last Updated:

09/01/2008

Links

Characterization Data

Gel

Click to enlarge image PAGE of Ag 10532 with molecular weight standards
Click image to enlarge

Ag 10532 Gel

PAGE of Ag 10532 with molecular weight standards

SOPs

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