MCM10, CPTAC-1210 - CPTAC Assay Portal
- ️ABCC's Scientific Web Programming Group, Frederick National Laboratory for Cancer Research (FNLCR)
Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.
Assay Details for CPTAC-1210 Collapse assay details
Data source: Panorama
-
Official Gene Symbol
- MCM10 Peptide Sequence
- GTNLIIQETR Modification Type
- unmodified Protein - Site of Modification
- N/A Peptide - Site of Modification
- N/A Peptide Start
- 494 Peptide End
- 503
-
CPTAC ID
- CPTAC-1210 Peptide Molecular Mass
- 1,143.6248 Species
- Homo sapiens (Human) Assay Type
- Direct MRM or SRM Matrix
- Ovarian cancer tumor tissue lysate Submitting Laboratory
- Pacific Northwest National Laboratory Submitting Lab PI
- Tao Liu
Assay Parameters Collapse assay parameters
Data source: Panorama
-
Instrument
- TSQ Vantage Internal Standard
- peptide Peptide Standard Purity
- >95% Peptide Standard Label Type
- 13C and 15N at C-terminus R
-
LC
- Waters nanoACQUITY (Part Number 176016000) Column Packing
- Waters BEH C18, 1.7 um 130 Å (Part Number 186007485) Column Dimensions
- 100 um x 100 mm Flow Rate
- 0.5 uL/min
Assay Multiplexing Expand assay panel
Pacific Northwest National Laboratory-DirectMRM
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y4 (1+) | 21 | 6.6 | 7.6 | 29.2 | 9.7 | 7.4 | 36 | 11.7 | 10.6 | 15 | 15 | 15 |
| y5 (1+) | 13.1 | 5.2 | 2.2 | 13 | 9.5 | 3.5 | 18.5 | 10.8 | 4.1 | 15 | 15 | 15 |
| y6 (1+) | 13.2 | 8.9 | 3.2 | 14.7 | 11.1 | 5.1 | 19.8 | 14.2 | 6 | 15 | 15 | 15 |
| sum | 8.9 | 4.5 | 2.5 | 10.2 | 8.7 | 4 | 13.5 | 9.8 | 4.7 | 15 | 15 | 15 |
Selectivity
Data source: Panorama
| Slope of Curve Fit for cell line | ||||||
|---|---|---|---|---|---|---|
| fragment_ion | 1 | 2 | 3 | 4 | 5 | 6 |
| y6 (1+) | 0.75 | 0.77 | 1.14 | 0.77 | 0.79 | 0.83 |
| y5 (1+) | 0.79 | 0.78 | 1.19 | 0.8 | 0.82 | 0.86 |
| y4 (1+) | 0.77 | 0.85 | 1.03 | 0.91 | 0.97 | 1.23 |
| sum | 0.77 | 0.78 | 1.15 | 0.8 | 0.82 | 0.89 |
Stability
Data source: Panorama
| Fragment ion / Transition | control_intra_CV | actual_temp_intra_CV | frozen_intra_CV | FTx1_intra_CV | FTx2_intra_CV |
|---|---|---|---|---|---|
| y4 (1+) | 13.6 | 9.1 | 16.8 | 7.8 | 5.8 |
| y5 (1+) | 3 | 4.3 | 1.7 | 1.4 | 5.5 |
| y6 (1+) | 4.2 | 6.9 | 2.5 | 7.5 | 3.1 |
| sum | 2.4 | 2.7 | 0.8 | 3 | 1.3 |
| Fragment ion / Transition | all_intra_CV | all_inter_CV |
|---|---|---|
| y4 (1+) | 10.4 | 12.9 |
| y5 (1+) | 3.4 | 6.3 |
| y6 (1+) | 5.2 | 5.6 |
| sum | 2.2 | 3.6 |
Endogenous
Data source: Panorama
| Fragment ion / Transition | intra_CV | inter_CV | total_CV |
|---|---|---|---|
| y6 (1+) | 50.2 | 42.2 | 65.6 |
| y5 (1+) | 52.2 | 56.9 | 77.2 |
| y4 (1+) | 47.7 | 45.9 | 66.2 |
| sum | 42.8 | 41.3 | 59.5 |
Additional Resources and Comments
Assay Details for CPTAC-1211 Collapse assay details
Data source: Panorama
-
Official Gene Symbol
- MCM10 Peptide Sequence
- IGGETLLPR Modification Type
- unmodified Protein - Site of Modification
- N/A Peptide - Site of Modification
- N/A Peptide Start
- 855 Peptide End
- 863
-
CPTAC ID
- CPTAC-1211 Peptide Molecular Mass
- 954.5498 Species
- Homo sapiens (Human) Assay Type
- Direct MRM or SRM Matrix
- Ovarian cancer tumor tissue lysate Submitting Laboratory
- Pacific Northwest National Laboratory Submitting Lab PI
- Tao Liu
Assay Parameters Collapse assay parameters
Data source: Panorama
-
Instrument
- TSQ Vantage Internal Standard
- peptide Peptide Standard Purity
- >95% Peptide Standard Label Type
- 13C and 15N at C-terminus R
-
LC
- Waters nanoACQUITY (Part Number 176016000) Column Packing
- Waters BEH C18, 1.7 um 130 Å (Part Number 186007485) Column Dimensions
- 100 um x 100 mm Flow Rate
- 0.5 uL/min
Assay Multiplexing Expand assay panel
Pacific Northwest National Laboratory-DirectMRM
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y5 (1+) | 16.8 | 7.5 | 6.3 | 21.9 | 8.8 | 8.7 | 27.6 | 11.6 | 10.7 | 15 | 15 | 15 |
| y7 (1+) | 21.4 | 6.9 | 6.4 | 23.1 | 8.3 | 5.7 | 31.5 | 10.8 | 8.6 | 15 | 15 | 15 |
| y8 (1+) | 14 | 5.3 | 4.5 | 18.7 | 9.5 | 5.8 | 23.4 | 10.9 | 7.3 | 15 | 15 | 15 |
| sum | 8 | 4.8 | 4.4 | 12.3 | 7.9 | 5 | 14.7 | 9.2 | 6.7 | 15 | 15 | 15 |
Selectivity
Data source: Panorama
| Slope of Curve Fit for cell line | ||||||
|---|---|---|---|---|---|---|
| fragment_ion | 1 | 2 | 3 | 4 | 5 | 6 |
| y8 (1+) | 1.19 | 1.23 | 1.84 | 1.25 | 1.33 | 1.55 |
| y7 (1+) | 1.31 | 1.4 | 1.85 | 1.37 | 1.37 | 1.65 |
| y5 (1+) | 1.23 | 1.15 | 1.67 | 1.07 | 1.4 | 1.59 |
| sum | 1.23 | 1.24 | 1.8 | 1.23 | 1.35 | 1.58 |
Stability
Data source: Panorama
| Fragment ion / Transition | control_intra_CV | actual_temp_intra_CV | frozen_intra_CV | FTx1_intra_CV | FTx2_intra_CV |
|---|---|---|---|---|---|
| Fragment ion / Transition | all_intra_CV | all_inter_CV |
|---|---|---|
Endogenous
Data source: Panorama
| Fragment ion / Transition | intra_CV | inter_CV | total_CV |
|---|---|---|---|
| y8 (1+) | 41.7 | 54.6 | 68.7 |
| y7 (1+) | 53 | 56.1 | 77.2 |
| y5 (1+) | 43.1 | 40.5 | 59.1 |
| sum | 34.8 | 37 | 50.8 |