CD63, CPTAC-1281 - CPTAC Assay Portal
- ️ABCC's Scientific Web Programming Group, Frederick National Laboratory for Cancer Research (FNLCR)
Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.
Assay Details for CPTAC-1281 Collapse assay details
Data source: Panorama
-
Official Gene Symbol
- CD63 Peptide Modified Sequence
- NN[+1.0]HTASILDR Modification Type
- Deamidated (N) Protein - Site of Modification
- 130 Peptide - Site of Modification
- 2 Peptide Start
- 129 Peptide End
- 138
-
CPTAC ID
- CPTAC-1281 Peptide Molecular Mass
- 1,140.5524 Species
- Homo sapiens (Human) Assay Type
- Direct MRM Matrix
- Human serum digest Submitting Laboratory
- Johns Hopkins University Submitting Lab PI
- Daniel Chan, Hui Zhang, Zhen Zhang
Assay Parameters Collapse assay parameters
Data source: Panorama
-
Instrument
- LCMS-8040 Internal Standard
- Stable isotope-labeled peptides Peptide Standard Purity
- Crude (~60%) Peptide Standard Label Type
- 13C and 15N at C-terminus R
-
LC
- Nexera X2 UHPLC Column Packing
- C18, 3 µm, 300 Å Column Dimensions
- 1.0 mm I.D. x 15 cm Flow Rate
- 50 µL/min
Assay Multiplexing Expand assay panel
Johns Hopkins University-C
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y5 (1+) | 52.2 | 23.1 | 26.8 | 68.6 | 25.4 | 30.1 | 86.2 | 34.3 | 40.3 | 14 | 15 | 15 |
| y6 (1+) | 47 | 10.1 | 21.6 | 65.3 | 19.2 | 24 | 80.5 | 21.7 | 32.3 | 15 | 15 | 15 |
| y7 (1+) | 34.6 | 25.2 | 8.4 | 45.6 | 24.3 | 12.9 | 57.2 | 35 | 15.4 | 15 | 15 | 15 |
| sum | 32.9 | 10.8 | 8.5 | 34.1 | 13.8 | 13.5 | 47.4 | 17.5 | 16 | 15 | 15 | 15 |