SRF, CPTAC-1727 - CPTAC Assay Portal
- ️ABCC's Scientific Web Programming Group, Frederick National Laboratory for Cancer Research (FNLCR)
Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.
Assay Details for CPTAC-1727 Collapse assay details
Data source: Panorama
-
Official Gene Symbol
- SRF Peptide Modified Sequence
- ALIQTC[+57.0]LNSPDS[+80.0]PPR Modification Type
- Carbamidomethyl (C), Phospho (ST) Protein - Site of Modification
- 218, 224 Peptide - Site of Modification
- 6, 12 Peptide Start
- 213 Peptide End
- 227
-
CPTAC ID
- CPTAC-1727 Peptide Molecular Mass
- 1,747.7964 Species
- Homo sapiens (Human) Assay Type
- Enrichment MRM or SRM Enrichment Method
- Titanium Dioxide Matrix
- Ovarian cancer cell lysate Submitting Laboratory
- Pacific Northwest National Laboratory Submitting Lab PI
- Tao Liu
Assay Parameters Collapse assay parameters
Data source: Panorama
-
Instrument
- TSQ Vantage Internal Standard
- peptide Peptide Standard Purity
- >95% Peptide Standard Label Type
- 13C and 15N at C-terminus R
-
LC
- Waters nanoACQUITY (Part Number 176016000) Column Packing
- Waters BEH C18, 1.7 um 130 Å (Part Number 186007485) Column Dimensions
- 100 um x 100 mm Flow Rate
- 0.5 uL/min
Assay Multiplexing Expand assay panel
Pacific Northwest National Laboratory-TiO2SRM
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y3 (1+) | 6.9 | 5.5 | 3.2 | 12.2 | 6.5 | 7.1 | 14 | 8.5 | 7.8 | 15 | 15 | 15 |
| y6 (1+) | 14.1 | 5 | 3.4 | 14.8 | 4.8 | 5.4 | 20.4 | 6.9 | 6.4 | 15 | 15 | 15 |
| y7 (1+) | 13.9 | 7.1 | 3.8 | 13.8 | 4.2 | 6.8 | 19.6 | 8.2 | 7.8 | 15 | 15 | 15 |
| sum | 6.9 | 4.3 | 1.9 | 9 | 4.7 | 5.7 | 11.3 | 6.4 | 6 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-1764 Collapse assay details
Data source: Panorama
-
Official Gene Symbol
- SRF Peptide Modified Sequence
- ALIQTC[+57.0]LNSPDSPPR Modification Type
- Carbamidomethyl (C) Protein - Site of Modification
- 218 Peptide - Site of Modification
- 6 Peptide Start
- 213 Peptide End
- 227
-
CPTAC ID
- CPTAC-1764 Peptide Molecular Mass
- 1,667.8301 Species
- Homo sapiens (Human) Assay Type
- Fractionation MRM or SRM Fractionation Approach
- N/A Matrix
- Ovarian cancer tumor tissue lysate Submitting Laboratory
- Pacific Northwest National Laboratory Submitting Lab PI
- Tao Liu
Assay Parameters Collapse assay parameters
Data source: Panorama
-
Instrument
- TSQ Vantage Internal Standard
- peptide Peptide Standard Purity
- >95% Peptide Standard Label Type
- 13C and 15N at C-terminus R
-
LC
- Waters nanoACQUITY (Part Number 176016000) Column Packing
- Waters BEH C18, 1.7 um 130 Å (Part Number 186007485) Column Dimensions
- 100 um x 100 mm Flow Rate
- 0.4 (uL/min)
Assay Multiplexing Expand assay panel
Pacific Northwest National Laboratory-TiO2SRM
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y4 (1+) | 4.4 | 3.7 | 3.9 | 5.8 | 5.4 | 5.6 | 7.3 | 6.5 | 6.8 | 15 | 15 | 15 |
| y6 (1+) | 3.8 | 3.8 | 4.1 | 5.1 | 3.6 | 3.6 | 6.4 | 5.2 | 5.5 | 15 | 15 | 15 |
| y8 (1+) | 2.5 | 3.6 | 6.7 | 6.4 | 3.5 | 7.3 | 6.9 | 5 | 9.9 | 15 | 15 | 15 |
| sum | 2.5 | 3.2 | 3.1 | 5 | 3.4 | 3.1 | 5.6 | 4.7 | 4.4 | 15 | 15 | 15 |