CTNNB1, CPTAC-1745 - CPTAC Assay Portal
- ️ABCC's Scientific Web Programming Group, Frederick National Laboratory for Cancer Research (FNLCR)
Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.
Protein Sequence hover to view complete sequence
| 10 | 20 | 30 | 40 | 50 |
| MATQADLMEL | DMAMEPDRKA | AVSHWQQQSY | LDSGIHSGAT | TTAPSLSGKG |
| 60 | 70 | 80 | 90 | 100 |
| NPEEEDVDTS | QVLYEWEQGF | SQSFTQEQVA | DIDGQYAMTR | AQRVRAAMFP |
| 110 | 120 | 130 | 140 | 150 |
| ETLDEGMQIP | STQFDAAHPT | NVQRLAEPSQ | MLKHAVVNLI | NYQDDAELAT |
| 160 | 170 | 180 | 190 | 200 |
| RAIPELTKLL | NDEDQVVVNK | AAVMVHQLSK | KEASRHAIMR | SPQMVSAIVR |
| 210 | 220 | 230 | 240 | 250 |
| TMQNTNDVET | ARCTAGTLHN | LSHHREGLLA | IFKSGGIPAL | VKMLGSPVDS |
| 260 | 270 | 280 | 290 | 300 |
| VLFYAITTLH | NLLLHQEGAK | MAVRLAGGLQ | KMVALLNKTN | VKFLAITTDC |
| 310 | 320 | 330 | 340 | 350 |
| LQILAYGNQE | SKLIILASGG | PQALVNIMRT | YTYEKLLWTT | SRVLKVLSVC |
| 360 | 370 | 380 | 390 | 400 |
| SSNKPAIVEA | GGMQALGLHL | TDPSQRLVQN | CLWTLRNLSD | AATKQEGMEG |
| 410 | 420 | 430 | 440 | 450 |
| LLGTLVQLLG | SDDINVVTCA | AGILSNLTCN | NYKNKMMVCQ | VGGIEALVRT |
| 460 | 470 | 480 | 490 | 500 |
| VLRAGDREDI | TEPAICALRH | LTSRHQEAEM | AQNAVRLHYG | LPVVVKLLHP |
| 510 | 520 | 530 | 540 | 550 |
| PSHWPLIKAT | VGLIRNLALC | PANHAPLREQ | GAIPRLVQLL | VRAHQDTQRR |
| 560 | 570 | 580 | 590 | 600 |
| TSMGGTQQQF | VEGVRMEEIV | EGCTGALHIL | ARDVHNRIVI | RGLNTIPLFV |
| 610 | 620 | 630 | 640 | 650 |
| QLLYSPIENI | QRVAAGVLCE | LAQDKEAAEA | IEAEGATAPL | TELLHSRNEG |
| 660 | 670 | 680 | 690 | 700 |
| VATYAAAVLF | RMSEDKPQDY | KKRLSVELTS | SLFRTEPMAW | NETADLGLDI |
| 710 | 720 | 730 | 740 | 750 |
| GAQGEPLGYR | QDDPSYRSFH | SGGYGQDALG | MDPMMEHEMG | GHHPGADYPV |
| 760 | 770 | 780 | 781 | |
| DGLPDLGHAQ | DLMDGLPPGD | SNQLAWFDTD | L |
Data source: UniProt
Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs
Uniprot Database Entry PhosphoSitePlus ®
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to view detailed assay information below
All other points link out to UniProt
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Assay Details for non-CPTAC-3265 Collapse assay details
Data source: Panorama
-
Official Gene Symbol
- CTNNB1 Peptide Sequence
- RTSMGGTQQQFVEGVR Modification Type
- unmodified Protein - Site of Modification
- N/A Peptide - Site of Modification
- N/A Peptide Start
- 550 Peptide End
- 565
-
CPTAC ID
- non-CPTAC-3265 Peptide Molecular Mass
- 1,779.8686 Species
- Homo sapiens (Human) Assay Type
- Direct MRM or SRM Matrix
- Cell Line Pool: H2444, H2122, AsPC-1, BxPC-3 Submitting Laboratory
- Moffitt Cancer Center Submitting Lab PI
- John Koomen
Publication
View Details (opens in a new window)
Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.
Assay Parameters Collapse assay parameters
Data source: Panorama
-
Instrument
- Thermo TSQ Quantiva Internal Standard
- light Peptide Standard Purity
- >95% Peptide Standard Label Type
- 13C and 15N at C-terminus R
-
LC
- Dionex Ultimate 3000 RSLCnano System Column Packing
- C18, 2 uM, 100 A Column Dimensions
- 75 uM x 25 cm Flow Rate
- 300 nl/min
Assay Multiplexing Expand assay panel
Pacific Northwest National Laboratory-TiO2SRM
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y7 (1+) | 11.1 | 4.8 | 1.6 | 15.1 | 12.5 | 7.4 | 18.7 | 13.4 | 7.6 | 15 | 15 | 15 |
| y6 (1+) | 7.2 | 3.8 | 2.6 | 13.7 | 10.6 | 8.4 | 15.5 | 11.3 | 8.8 | 15 | 15 | 15 |
| y5 (1+) | 9.7 | 3.7 | 3.1 | 11.2 | 9.7 | 7.5 | 14.8 | 10.4 | 8.1 | 15 | 15 | 15 |
| y4 (1+) | 7 | 3 | 2.3 | 10 | 9.7 | 9.9 | 12.2 | 10.2 | 10.2 | 15 | 15 | 15 |
| sum | 4.6 | 3.1 | 1.3 | 10.3 | 10 | 8.2 | 11.3 | 10.5 | 8.3 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for non-CPTAC-5716 Collapse assay details
Data source: Panorama
-
Official Gene Symbol
- CTNNB1 Peptide Modified Sequence
- RTS[+79.966331]MGGTQQQFVEGVR Modification Type
- Phospho (ST) Protein - Site of Modification
- N/A Peptide - Site of Modification
- 3 Peptide Start
- 550 Peptide End
- 565
-
CPTAC ID
- non-CPTAC-5716 Peptide Molecular Mass
- 1,859.8349 Species
- Homo sapiens (Human) Assay Type
- Enrichment MRM or SRM Enrichment Method
- Titanium Dioxide Matrix
- Cell Lysate Submitting Laboratory
- Moffitt Cancer Center Submitting Lab PI
- John Koomen
Publication
View Details (opens in a new window)
Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.
Assay Parameters Collapse assay parameters
Data source: Panorama
-
Instrument
- Thermo Quantiva Internal Standard
- peptide Peptide Standard Purity
- >95% Peptide Standard Label Type
- 13C and 15N at C-terminus R
-
LC
- Dionex Ultimate 3000 RSLCnano Column Packing
- Pepmap 100 Column Dimensions
- 75um x 25cm Flow Rate
- 300 nL/min
Assay Multiplexing Expand assay panel
Pacific Northwest National Laboratory-TiO2SRM
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y3 (1+) | 4.4 | 3.4 | 2.3 | 6.1 | 3.9 | 3.1 | 7.5 | 5.2 | 3.9 | 15 | 15 | 15 |
| y4 (1+) | 5.6 | 2.9 | 2.6 | 6 | 4 | 2.6 | 8.2 | 4.9 | 3.7 | 15 | 15 | 15 |
| y6 (1+) | 2.6 | 3.2 | 3.2 | 2.3 | 3.6 | 3.4 | 3.5 | 4.8 | 4.7 | 15 | 15 | 15 |
| y7 (1+) | 7 | 5.2 | 2.5 | 5.4 | 4.8 | 3.1 | 8.8 | 7.1 | 4 | 15 | 15 | 15 |
| sum | 2.7 | 2.1 | 2.1 | 2.4 | 2.7 | 2.4 | 3.6 | 3.4 | 3.2 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for non-CPTAC-3266 Collapse assay details
Data source: Panorama
-
Official Gene Symbol
- CTNNB1 Peptide Sequence
- TSMGGTQQQFVEGVR Modification Type
- unmodified Protein - Site of Modification
- N/A Peptide - Site of Modification
- N/A Peptide Start
- 551 Peptide End
- 565
-
CPTAC ID
- non-CPTAC-3266 Peptide Molecular Mass
- 1,623.7675 Species
- Homo sapiens (Human) Assay Type
- Direct MRM or SRM Matrix
- Cell Line Pool: H2444, H2122, AsPC-1, BxPC-3 Submitting Laboratory
- Moffitt Cancer Center Submitting Lab PI
- John Koomen
Publication
View Details (opens in a new window)
Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.
Assay Parameters Collapse assay parameters
Data source: Panorama
-
Instrument
- Thermo TSQ Quantiva Internal Standard
- light Peptide Standard Purity
- >95% Peptide Standard Label Type
- 13C and 15N at C-terminus R
-
LC
- Dionex Ultimate 3000 RSLCnano System Column Packing
- C18, 2 uM, 100 A Column Dimensions
- 75 uM x 25 cm Flow Rate
- 300 nl/min
Assay Multiplexing Expand assay panel
Pacific Northwest National Laboratory-TiO2SRM
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y8 (1+) | 9.9 | 3.4 | 1.6 | 11.8 | 9.3 | 10 | 15.4 | 9.9 | 10.1 | 15 | 15 | 15 |
| y7 (1+) | 8.1 | 4.3 | 2.4 | 14.6 | 10.2 | 9.2 | 16.7 | 11.1 | 9.5 | 15 | 15 | 15 |
| y6 (1+) | 8.3 | 4.2 | 2.4 | 15.2 | 10.9 | 10.1 | 17.3 | 11.7 | 10.4 | 15 | 15 | 15 |
| y4 (1+) | 7.3 | 2.5 | 1.3 | 15.4 | 10.2 | 8.5 | 17 | 10.5 | 8.6 | 15 | 15 | 15 |
| y3 (1+) | 5.3 | 2.7 | 1.4 | 11.6 | 10.4 | 9.1 | 12.8 | 10.7 | 9.2 | 15 | 15 | 15 |
| sum | 1.9 | 1.8 | 0.9 | 12.2 | 10 | 9 | 12.3 | 10.2 | 9 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for non-CPTAC-5365 Collapse assay details
Data source: Panorama
-
Official Gene Symbol
- CTNNB1 Peptide Sequence
- TSMGGTQQQFVEGVR Modification Type
- unmodified Protein - Site of Modification
- N/A Peptide - Site of Modification
- N/A Peptide Start
- 551 Peptide End
- 565
-
CPTAC ID
- non-CPTAC-5365 Peptide Molecular Mass
- 1,623.7675 Species
- Homo sapiens (Human) Assay Type
- Direct MRM or SRM Matrix
- Cell Lysate Submitting Laboratory
- Moffitt Cancer Center Submitting Lab PI
- John Koomen
Publication
View Details (opens in a new window)
Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.
Assay Parameters Collapse assay parameters
Data source: Panorama
-
Instrument
- RSLCnano and Quantiva Internal Standard
- Stable Isotope Labeled Standard Peptide Standard Purity
- >95% Peptide Standard Label Type
- 13C and 15N at C-terminus R
-
LC
- RSLCnano Column Packing
- C18 PepMap 100 Column Dimensions
- 75 micron ID x 25 cm length Flow Rate
- 300 nl/min
Assay Multiplexing Expand assay panel
Pacific Northwest National Laboratory-TiO2SRM
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y4 (1+) | 8.1 | 3.3 | 2.8 | 8.7 | 4 | 2.9 | 11.9 | 5.2 | 4 | 15 | 15 | 15 |
| y6 (1+) | 6.8 | 2.8 | 3.1 | 9.8 | 3.1 | 4.5 | 11.9 | 4.2 | 5.5 | 15 | 15 | 15 |
| y7 (1+) | 8.9 | 3.8 | 3.4 | 9.7 | 5 | 3.3 | 13.2 | 6.3 | 4.7 | 15 | 15 | 15 |
| y8 (1+) | 12.2 | 4.4 | 3.4 | 13.3 | 3.4 | 4.8 | 18 | 5.6 | 5.9 | 15 | 15 | 15 |
| y3 (1+) | 4.9 | 2.3 | 1.6 | 7.4 | 3 | 2.6 | 8.9 | 3.8 | 3.1 | 15 | 15 | 15 |
| sum | 4.8 | 1.2 | 1.7 | 6.1 | 2.2 | 2.8 | 7.8 | 2.5 | 3.3 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for non-CPTAC-5715 Collapse assay details
Data source: Panorama
-
Official Gene Symbol
- CTNNB1 Peptide Modified Sequence
- TS[+79.966331]MGGTQQQFVEGVR Modification Type
- Phospho (ST) Protein - Site of Modification
- N/A Peptide - Site of Modification
- 3 Peptide Start
- 551 Peptide End
- 565
-
CPTAC ID
- non-CPTAC-5715 Peptide Molecular Mass
- 1,703.7338 Species
- Homo sapiens (Human) Assay Type
- Enrichment MRM or SRM Enrichment Method
- Titanium Dioxide Matrix
- Cell Lysate Submitting Laboratory
- Moffitt Cancer Center Submitting Lab PI
- John Koomen
Publication
View Details (opens in a new window)
Targeted mass spectrometry-based assays enable multiplex quantification of receptor tyrosine kinase, MAP Kinase, and AKT signaling. Whiteaker JR, Sharma K, Hoffman MA, Kuhn E, Zhao L, Cocco AR, Schoenherr RM, Kennedy JJ, Voytovich U, Lin C, Fang B, Bowers K, Whiteley G, Colantonio S, Bocik W, Roberts R, Hiltke T, Boja E, Rodriguez H, McCormick F, Holderfield M, Carr SA, Koomen JM, Paulovich AG. Cell Reports Methods doi: 10.1016/j.crmeth.2021.100015.
Assay Parameters Collapse assay parameters
Data source: Panorama
-
Instrument
- Thermo Quantiva Internal Standard
- peptide Peptide Standard Purity
- >95% Peptide Standard Label Type
- 13C and 15N at C-terminus R
-
LC
- Dionex Ultimate 3000 RSLCnano Column Packing
- Pepmap 100 Column Dimensions
- 75um x 25cm Flow Rate
- 300 nL/min
Assay Multiplexing Expand assay panel
Pacific Northwest National Laboratory-TiO2SRM
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y6 (1+) | 10.1 | 4.8 | 3.3 | 9.7 | 5.3 | 4.5 | 14 | 7.2 | 5.6 | 15 | 15 | 15 |
| y4 (1+) | 8.5 | 4.8 | 2.6 | 8.9 | 6.6 | 5.4 | 12.3 | 8.2 | 6 | 15 | 15 | 15 |
| y5 (1+) | 7.5 | 5.7 | 1.9 | 6.9 | 7.4 | 3.4 | 10.2 | 9.3 | 3.9 | 15 | 15 | 15 |
| y8 (1+) | 11.2 | 4.3 | 3.1 | 10.7 | 4.8 | 3.9 | 15.5 | 6.4 | 5 | 15 | 15 | 15 |
| sum | 5.7 | 2.6 | 1.6 | 6.7 | 3.2 | 4 | 8.8 | 4.1 | 4.3 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-1745 Collapse assay details
Data source: Panorama
-
Official Gene Symbol
- CTNNB1 Peptide Modified Sequence
- S[+80.0]PQMVSAIVR Modification Type
- Phospho (ST) Protein - Site of Modification
- 191 Peptide - Site of Modification
- 1 Peptide Start
- 191 Peptide End
- 200
-
CPTAC ID
- CPTAC-1745 Peptide Molecular Mass
- 1,166.5519 Species
- Homo sapiens (Human) Assay Type
- Enrichment MRM or SRM Enrichment Method
- Titanium Dioxide Matrix
- Ovarian cancer cell lysate Submitting Laboratory
- Pacific Northwest National Laboratory Submitting Lab PI
- Tao Liu
Assay Parameters Collapse assay parameters
Data source: Panorama
-
Instrument
- TSQ Vantage Internal Standard
- peptide Peptide Standard Purity
- >95% Peptide Standard Label Type
- 13C and 15N at C-terminus R
-
LC
- Waters nanoACQUITY (Part Number 176016000) Column Packing
- Waters BEH C18, 1.7 um 130 Å (Part Number 186007485) Column Dimensions
- 100 um x 100 mm Flow Rate
- 0.5 uL/min
Assay Multiplexing Expand assay panel
Pacific Northwest National Laboratory-TiO2SRM
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y5 (1+) | 23.6 | 5.4 | 7.2 | 26.3 | 6.2 | 12.1 | 35.3 | 8.2 | 14.1 | 15 | 15 | 15 |
| y6 (1+) | 23.1 | 9.1 | 7 | 21.6 | 10.9 | 9.9 | 31.6 | 14.2 | 12.1 | 15 | 15 | 15 |
| y7 (1+) | 17.9 | 6.8 | 3.9 | 18.7 | 6.9 | 5.2 | 25.9 | 9.7 | 6.5 | 15 | 15 | 15 |
| sum | 14.5 | 4 | 3.1 | 15.8 | 5.1 | 4.9 | 21.4 | 6.5 | 5.8 | 15 | 15 | 15 |