FOSB, CPTAC-1760 - CPTAC Assay Portal
- ️ABCC's Scientific Web Programming Group, Frederick National Laboratory for Cancer Research (FNLCR)
Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.
Protein Sequence hover to view complete sequence
| 10 | 20 | 30 | 40 | 50 |
| MFQAFPGDYD | SGSRCSSSPS | AESQYLSSVD | SFGSPPTAAA | SQECAGLGEM |
| 60 | 70 | 80 | 90 | 100 |
| PGSFVPTVTA | ITTSQDLQWL | VQPTLISSMA | QSQGQPLASQ | PPVVDPYDMP |
| 110 | 120 | 130 | 140 | 150 |
| GTSYSTPGMS | GYSSGGASGS | GGPSTSGTTS | GPGPARPARA | RPRRPREETL |
| 160 | 170 | 180 | 190 | 200 |
| TPEEEEKRRV | RRERNKLAAA | KCRNRRRELT | DRLQAETDQL | EEEKAELESE |
| 210 | 220 | 230 | 240 | 250 |
| IAELQKEKER | LEFVLVAHKP | GCKIPYEEGP | GPGPLAEVRD | LPGSAPAKED |
| 260 | 270 | 280 | 290 | 300 |
| GFSWLLPPPP | PPPLPFQTSQ | DAPPNLTASL | FTHSEVQVLG | DPFPVVNPSY |
| 310 | 320 | 330 | 338 | |
| TSSFVLTCPE | VSAFAGAQRT | SGSDQPSDPL | NSPSLLAL |
Data source: UniProt
Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs
Uniprot Database Entry PhosphoSitePlus ®
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Assay Details for CPTAC-1760 Collapse assay details
Data source: Panorama
-
Official Gene Symbol
- FOSB Peptide Sequence
- IPYEEGPGPGPLAEVR Modification Type
- unmodified Protein - Site of Modification
- N/A Peptide - Site of Modification
- N/A Peptide Start
- 224 Peptide End
- 239
-
CPTAC ID
- CPTAC-1760 Peptide Molecular Mass
- 1,679.8519 Species
- Homo sapiens (Human) Assay Type
- Fractionation PRISM-SRM Fractionation Approach
- high-pressure, high-resolution, high pH reverse phase frationation with online monitoring (PRISM) Matrix
- Ovarian cancer tumor tissue lysate Submitting Laboratory
- Pacific Northwest National Laboratory Submitting Lab PI
- Tao Liu
Assay Parameters Collapse assay parameters
Data source: Panorama
-
Instrument
- TSQ Vantage Internal Standard
- peptide Peptide Standard Purity
- >95% Peptide Standard Label Type
- 13C and 15N at C-terminus R
-
LC
- Waters nanoACQUITY (Part Number 176016000) Column Packing
- Waters BEH C18, 1.7 um 130 Å (Part Number 186007485) Column Dimensions
- 100 um x 100 mm Flow Rate
- 0.4 (uL/min)
Assay Multiplexing Expand assay panel
Pacific Northwest National Laboratory-PRISMSRM
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y10 (1+) | 2.8 | 2.9 | 1.7 | 3.5 | 5.3 | 1.9 | 4.5 | 6 | 2.5 | 15 | 15 | 15 |
| y11 (1+) | 2.8 | 2.3 | 2.4 | 2 | 2.2 | 2.6 | 3.4 | 3.2 | 3.5 | 15 | 15 | 15 |
| y12 (1+) | 5.1 | 2.3 | 2.2 | 5.5 | 2.8 | 3.4 | 7.5 | 3.6 | 4 | 15 | 15 | 15 |
| sum | 2.3 | 1.4 | 1.5 | 2.3 | 2.9 | 1.8 | 3.3 | 3.2 | 2.3 | 15 | 15 | 15 |