CA2, CPTAC-177 - CPTAC Assay Portal
- ️ABCC's Scientific Web Programming Group, Frederick National Laboratory for Cancer Research (FNLCR)
Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.
Protein Sequence hover to view complete sequence
| 10 | 20 | 30 | 40 | 50 |
| MSHHWGYGKH | NGPEHWHKDF | PIAKGERQSP | VDIDTHTAKY | DPSLKPLSVS |
| 60 | 70 | 80 | 90 | 100 |
| YDQATSLRIL | NNGHAFNVEF | DDSQDKAVLK | GGPLDGTYRL | IQFHFHWGSL |
| 110 | 120 | 130 | 140 | 150 |
| DGQGSEHTVD | KKKYAAELHL | VHWNTKYGDF | GKAVQQPDGL | AVLGIFLKVG |
| 160 | 170 | 180 | 190 | 200 |
| SAKPGLQKVV | DVLDSIKTKG | KSADFTNFDP | RGLLPESLDY | WTYPGSLTTP |
| 210 | 220 | 230 | 240 | 250 |
| PLLECVTWIV | LKEPISVSSE | QVLKFRKLNF | NGEGEPEELM | VDNWRPAQPL |
| 260 | ||||
| KNRQIKASFK |
Data source: UniProt
Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs
Uniprot Database Entry PhosphoSitePlus ®
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Assay Details for CPTAC-178 Collapse assay details
Data source: Panorama
-
Official Gene Symbol
- CA2 Peptide Sequence
- SADFTNFDPR Modification Type
- unmodified Protein - Site of Modification
- N/A Peptide - Site of Modification
- N/A Peptide Start
- 172 Peptide End
- 181
-
CPTAC ID
- CPTAC-178 Peptide Molecular Mass
- 1,168.5149 Species
- Homo sapiens (Human) Assay Type
- Direct MRM Matrix
- cell line lysate pool Submitting Laboratory
- Fred Hutchinson Cancer Research Center Submitting Lab PI
- Amanda Paulovich
Publication
View Details (opens in a new window)
Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253
Assay Parameters Collapse assay parameters
Data source: Panorama
-
Instrument
- 5500 QTRAP (ABSCIEX) Internal Standard
- peptide Peptide Standard Purity
- >95% Peptide Standard Label Type
- 13C and 15N R
-
LC
- nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent) Column Packing
- ChromXP C18-CL, 3 um, 120A Column Dimensions
- 150 x 0.075 mm Flow Rate
- 500 nL / min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y8 (1+) | 6.1 | 9.7 | 6.9 | 13.3 | 13 | 10 | 14.6 | 16.2 | 12.1 | 15 | 25 | 15 |
| y6 (1+) | 6.8 | 7.6 | 7.6 | 9.2 | 9.7 | 8.1 | 11.4 | 12.3 | 11.1 | 15 | 25 | 15 |
| y5 (1+) | 9.2 | 9 | 7.3 | 10.7 | 10 | 9.8 | 14.1 | 13.5 | 12.2 | 15 | 25 | 15 |
| sum | 4.6 | 6.8 | 5.5 | 7.4 | 8.7 | 4.9 | 8.7 | 11 | 7.4 | 15 | 25 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-177 Collapse assay details
Data source: Panorama
-
Official Gene Symbol
- CA2 Peptide Sequence
- VVDVLDSIK Modification Type
- unmodified Protein - Site of Modification
- N/A Peptide - Site of Modification
- N/A Peptide Start
- 159 Peptide End
- 167
-
CPTAC ID
- CPTAC-177 Peptide Molecular Mass
- 986.5648 Species
- Homo sapiens (Human) Assay Type
- Direct MRM Matrix
- cell line lysate pool Submitting Laboratory
- Fred Hutchinson Cancer Research Center Submitting Lab PI
- Amanda Paulovich
Publication
View Details (opens in a new window)
Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253
Assay Parameters Collapse assay parameters
Data source: Panorama
-
Instrument
- 5500 QTRAP (ABSCIEX) Internal Standard
- peptide Peptide Standard Purity
- >95% Peptide Standard Label Type
- 13C and 15N K
-
LC
- nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent) Column Packing
- ChromXP C18-CL, 3 um, 120A Column Dimensions
- 150 x 0.075 mm Flow Rate
- 500 nL / min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y7 (1+) | 9.5 | 13.6 | 9.9 | 19 | 21.2 | 24 | 21.2 | 25.2 | 26 | 15 | 25 | 15 |
| y6 (1+) | 16.6 | 16.3 | 9.9 | 16.1 | 17.5 | 9.8 | 23.1 | 23.9 | 13.9 | 15 | 25 | 15 |
| y5 (1+) | 10 | 10.5 | 13.6 | 7.9 | 12.3 | 12.3 | 12.7 | 16.2 | 18.3 | 15 | 25 | 15 |
| sum | 7.7 | 11.3 | 7.7 | 14.8 | 16.4 | 17.7 | 16.7 | 19.9 | 19.3 | 15 | 25 | 15 |
Additional Resources and Comments
Assay Details for non-CPTAC-3365 Collapse assay details
Data source: Panorama
-
Official Gene Symbol
- Ca2 Peptide Sequence
- GGPLSDSYR Modification Type
- unmodified Protein - Site of Modification
- N/A Peptide - Site of Modification
- N/A Peptide Start
- 81 Peptide End
- 89
-
CPTAC ID
- non-CPTAC-3365 Peptide Molecular Mass
- 950.4458 Species
- Mus musculus (Mouse) Assay Type
- Direct MRM Matrix
- Plasma Submitting Laboratory
- UVic-Genome BC Proteomics Centre Submitting Lab PI
- Christoph Borchers
Assay Parameters Collapse assay parameters
Data source: Panorama
-
Instrument
- Agilent 6490/6495 QQQ Internal Standard
- synthetic peptide Peptide Standard Purity
- >95% Peptide Standard Label Type
- 13C and 15N at C-terminus R
-
LC
- 1290 LC (Agilent) Column Packing
- Zorbax Eclipse Plus C18, 1.8 µm Column Dimensions
- 2.1 x 150 mm Flow Rate
- 400 µL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| b3 (1+) | 4.9 | 8 | 4.9 | 10.2 | 9.1 | 8.5 | 11.3 | 12.1 | 9.8 | 15 | 15 | 15 |
| y7 (2+) | 6.9 | 4.1 | 3.6 | 12 | 7.7 | 6.9 | 13.8 | 8.7 | 7.8 | 15 | 15 | 15 |
| y3 (1+) | 5.8 | 4.8 | 3.9 | 14.4 | 8.9 | 4.4 | 15.5 | 10.1 | 5.9 | 15 | 15 | 15 |
| y4 (1+) | 7.8 | 5.2 | 6.9 | 12.3 | 10 | 9.7 | 14.6 | 11.3 | 11.9 | 15 | 15 | 15 |
| y5 (1+) | 3.2 | 3.6 | 5.5 | 12.6 | 9.8 | 9.4 | 13 | 10.4 | 10.9 | 15 | 15 | 15 |
| sum | 3.1 | 2.5 | 2.1 | 11.3 | 7.1 | 5.9 | 11.7 | 7.5 | 6.3 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for non-CPTAC-3303 Collapse assay details
Data source: Panorama
-
Official Gene Symbol
- Ca2 Peptide Sequence
- IGPASQGLQK Modification Type
- unmodified Protein - Site of Modification
- N/A Peptide - Site of Modification
- N/A Peptide Start
- 149 Peptide End
- 158
-
CPTAC ID
- non-CPTAC-3303 Peptide Molecular Mass
- 997.5556 Species
- Mus musculus (Mouse) Assay Type
- Direct MRM Matrix
- Plasma Submitting Laboratory
- UVic-Genome BC Proteomics Centre Submitting Lab PI
- Christoph Borchers
Publication
View Details (opens in a new window)
Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.
Assay Parameters Collapse assay parameters
Data source: Panorama
-
Instrument
- Agilent 6490/6495 QQQ Internal Standard
- synthetic peptide Peptide Standard Purity
- >95% Peptide Standard Label Type
- 13C and 15N at C-terminus K
-
LC
- 1290 LC (Agilent) Column Packing
- Zorbax Eclipse Plus C18, 1.8 µm Column Dimensions
- 2.1 x 150 mm Flow Rate
- 400 µL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y9 (1+) | 9.9 | 5.1 | 6.9 | 14.5 | 9.1 | 7 | 17.6 | 10.4 | 9.8 | 15 | 15 | 15 |
| y6 (1+) | 11 | 6.6 | 4.4 | 27.5 | 8.7 | 5.6 | 29.6 | 10.9 | 7.1 | 15 | 15 | 15 |
| y7 (1+) | 9.7 | 5.7 | 6.2 | 11.5 | 6.6 | 5.7 | 15 | 8.7 | 8.4 | 15 | 15 | 15 |
| y9 (2+) | 13.8 | 6.8 | 4.1 | 15.4 | 9.5 | 5 | 20.7 | 11.7 | 6.5 | 15 | 15 | 15 |
| y8 (2+) | 5.6 | 5.1 | 5.8 | 19.1 | 7.2 | 6.4 | 19.9 | 8.8 | 8.6 | 15 | 15 | 15 |
| sum | 3.7 | 3 | 3.6 | 14.2 | 6.4 | 4.3 | 14.7 | 7.1 | 5.6 | 15 | 15 | 15 |