ACE, CPTAC-2597 - CPTAC Assay Portal
- ️ABCC's Scientific Web Programming Group, Frederick National Laboratory for Cancer Research (FNLCR)
Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.
Protein Sequence hover to view complete sequence
| 10 | 20 | 30 | 40 | 50 |
| MGAASGRRGP | GLLLPLPLLL | LLPPQPALAL | DPGLQPGNFS | ADEAGAQLFA |
| 60 | 70 | 80 | 90 | 100 |
| QSYNSSAEQV | LFQSVAASWA | HDTNITAENA | RRQEEAALLS | QEFAEAWGQK |
| 110 | 120 | 130 | 140 | 150 |
| AKELYEPIWQ | NFTDPQLRRI | IGAVRTLGSA | NLPLAKRQQY | NALLSNMSRI |
| 160 | 170 | 180 | 190 | 200 |
| YSTAKVCLPN | KTATCWSLDP | DLTNILASSR | SYAMLLFAWE | GWHNAAGIPL |
| 210 | 220 | 230 | 240 | 250 |
| KPLYEDFTAL | SNEAYKQDGF | TDTGAYWRSW | YNSPTFEDDL | EHLYQQLEPL |
| 260 | 270 | 280 | 290 | 300 |
| YLNLHAFVRR | ALHRRYGDRY | INLRGPIPAH | LLGDMWAQSW | ENIYDMVVPF |
| 310 | 320 | 330 | 340 | 350 |
| PDKPNLDVTS | TMLQQGWNAT | HMFRVAEEFF | TSLELSPMPP | EFWEGSMLEK |
| 360 | 370 | 380 | 390 | 400 |
| PADGREVVCH | ASAWDFYNRK | DFRIKQCTRV | TMDQLSTVHH | EMGHIQYYLQ |
| 410 | 420 | 430 | 440 | 450 |
| YKDLPVSLRR | GANPGFHEAI | GDVLALSVST | PEHLHKIGLL | DRVTNDTESD |
| 460 | 470 | 480 | 490 | 500 |
| INYLLKMALE | KIAFLPFGYL | VDQWRWGVFS | GRTPPSRYNF | DWWYLRTKYQ |
| 510 | 520 | 530 | 540 | 550 |
| GICPPVTRNE | THFDAGAKFH | VPNVTPYIRY | FVSFVLQFQF | HEALCKEAGY |
| 560 | 570 | 580 | 590 | 600 |
| EGPLHQCDIY | RSTKAGAKLR | KVLQAGSSRP | WQEVLKDMVG | LDALDAQPLL |
| 610 | 620 | 630 | 640 | 650 |
| KYFQPVTQWL | QEQNQQNGEV | LGWPEYQWHP | PLPDNYPEGI | DLVTDEAEAS |
| 660 | 670 | 680 | 690 | 700 |
| KFVEEYDRTS | QVVWNEYAEA | NWNYNTNITT | ETSKILLQKN | MQIANHTLKY |
| 710 | 720 | 730 | 740 | 750 |
| GTQARKFDVN | QLQNTTIKRI | IKKVQDLERA | ALPAQELEEY | NKILLDMETT |
| 760 | 770 | 780 | 790 | 800 |
| YSVATVCHPN | GSCLQLEPDL | TNVMATSRKY | EDLLWAWEGW | RDKAGRAILQ |
| 810 | 820 | 830 | 840 | 850 |
| FYPKYVELIN | QAARLNGYVD | AGDSWRSMYE | TPSLEQDLER | LFQELQPLYL |
| 860 | 870 | 880 | 890 | 900 |
| NLHAYVRRAL | HRHYGAQHIN | LEGPIPAHLL | GNMWAQTWSN | IYDLVVPFPS |
| 910 | 920 | 930 | 940 | 950 |
| APSMDTTEAM | LKQGWTPRRM | FKEADDFFTS | LGLLPVPPEF | WNKSMLEKPT |
| 960 | 970 | 980 | 990 | 1000 |
| DGREVVCHAS | AWDFYNGKDF | RIKQCTTVNL | EDLVVAHHEM | GHIQYFMQYK |
| 1010 | 1020 | 1030 | 1040 | 1050 |
| DLPVALREGA | NPGFHEAIGD | VLALSVSTPK | HLHSLNLLSS | EGGSDEHDIN |
| 1060 | 1070 | 1080 | 1090 | 1100 |
| FLMKMALDKI | AFIPFSYLVD | QWRWRVFDGS | ITKENYNQEW | WSLRLKYQGL |
| 1110 | 1120 | 1130 | 1140 | 1150 |
| CPPVPRTQGD | FDPGAKFHIP | SSVPYIRYFV | SFIIQFQFHE | ALCQAAGHTG |
| 1160 | 1170 | 1180 | 1190 | 1200 |
| PLHKCDIYQS | KEAGQRLATA | MKLGFSRPWP | EAMQLITGQP | NMSASAMLSY |
| 1210 | 1220 | 1230 | 1240 | 1250 |
| FKPLLDWLRT | ENELHGEKLG | WPQYNWTPNS | ARSEGPLPDS | GRVSFLGLDL |
| 1260 | 1270 | 1280 | 1290 | 1300 |
| DAQQARVGQW | LLLFLGIALL | VATLGLSQRL | FSIRHRSLHR | HSHGPQFGSE |
| 1306 | ||||
| VELRHS |
Data source: UniProt
Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs
Uniprot Database Entry PhosphoSitePlus ®
Click a point on a node
to view detailed assay information below
All other points link out to UniProt
loading
Assay Details for CPTAC-2597 Collapse assay details
Data source: Panorama
-
Official Gene Symbol
- ACE Peptide Modified Sequence
- ELYEPIWQN[+1.0]FTDPQLR Modification Type
- Deamidated (N) Protein - Site of Modification
- 111 Peptide - Site of Modification
- 9 Peptide Start
- 103 Peptide End
- 118
-
CPTAC ID
- CPTAC-2597 Peptide Molecular Mass
- 2,048.9844 Species
- Homo sapiens (Human) Assay Type
- Direct MRM Matrix
- Human ovarian tumor tissue digest Submitting Laboratory
- Johns Hopkins University Submitting Lab PI
- Daniel Chan, Hui Zhang, Zhen Zhang
Assay Parameters Collapse assay parameters
Data source: Panorama
-
Instrument
- TSQ Vantage Internal Standard
- Stable isotope-labeled peptides Peptide Standard Purity
- Crude (~60%) Peptide Standard Label Type
- 13C and 15N at C-terminus R
-
LC
- Accela 1250 Quaternary Low Pump Column Packing
- C18, 3 µm, 300 Å Column Dimensions
- 1.0 mm I.D. x 15 cm Flow Rate
- 50 µL/min
Assay Multiplexing Expand assay panel
Johns Hopkins University-I
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y8 (1+) | 60.9 | 14.4 | 13.3 | 70.4 | 15.8 | 16.5 | 93.1 | 21.4 | 21.2 | 15 | 15 | 15 |
| y5 (1+) | 43.9 | 19.9 | 14.6 | 44.6 | 19.8 | 16.9 | 62.6 | 28.1 | 22.3 | 15 | 15 | 15 |
| y4 (1+) | 26.3 | 7.4 | 7.7 | 24.9 | 11.4 | 9.3 | 36.2 | 13.6 | 12.1 | 15 | 15 | 15 |
| sum | 25.9 | 5.7 | 8.1 | 23.5 | 10.4 | 9.7 | 35 | 11.9 | 12.6 | 15 | 15 | 15 |