ATR, CPTAC-3214 - CPTAC Assay Portal
- ️ABCC's Scientific Web Programming Group, Frederick National Laboratory for Cancer Research (FNLCR)
Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.
Protein Sequence hover to view complete sequence
| 10 | 20 | 30 | 40 | 50 |
| MGEHGLELAS | MIPALRELGS | ATPEEYNTVV | QKPRQILCQF | IDRILTDVNV |
| 60 | 70 | 80 | 90 | 100 |
| VAVELVKKTD | SQPTSVMLLD | FIQHIMKSSP | LMFVNVSGSH | EAKGSCIEFS |
| 110 | 120 | 130 | 140 | 150 |
| NWIITRLLRI | AATPSCHLLH | KKICEVICSL | LFLFKSKSPA | IFGVLTKELL |
| 160 | 170 | 180 | 190 | 200 |
| QLFEDLVYLH | RRNVMGHAVE | WPVVMSRFLS | QLDEHMGYLQ | SAPLQLMSMQ |
| 210 | 220 | 230 | 240 | 250 |
| NLEFIEVTLL | MVLTRIIAIV | FFRRQELLLW | QIGCVLLEYG | SPKIKSLAIS |
| 260 | 270 | 280 | 290 | 300 |
| FLTELFQLGG | LPAQPASTFF | SSFLELLKHL | VEMDTDQLKL | YEEPLSKLIK |
| 310 | 320 | 330 | 340 | 350 |
| TLFPFEAEAY | RNIEPVYLNM | LLEKLCVMFE | DGVLMRLKSD | LLKAALCHLL |
| 360 | 370 | 380 | 390 | 400 |
| QYFLKFVPAG | YESALQVRKV | YVRNICKALL | DVLGIEVDAE | YLLGPLYAAL |
| 410 | 420 | 430 | 440 | 450 |
| KMESMEIIEE | IQCQTQQENL | SSNSDGISPK | RRRLSSSLNP | SKRAPKQTEE |
| 460 | 470 | 480 | 490 | 500 |
| IKHVDMNQKS | ILWSALKQKA | ESLQISLEYS | GLKNPVIEML | EGIAVVLQLT |
| 510 | 520 | 530 | 540 | 550 |
| ALCTVHCSHQ | NMNCRTFKDC | QHKSKKKPSV | VITWMSLDFY | TKVLKSCRSL |
| 560 | 570 | 580 | 590 | 600 |
| LESVQKLDLE | ATIDKVVKIY | DALIYMQVNS | SFEDHILEDL | CGMLSLPWIY |
| 610 | 620 | 630 | 640 | 650 |
| SHSDDGCLKL | TTFAANLLTL | SCRISDSYSP | QAQSRCVFLL | TLFPRRIFLE |
| 660 | 670 | 680 | 690 | 700 |
| WRTAVYNWAL | QSSHEVIRAS | CVSGFFILLQ | QQNSCNRVPK | ILIDKVKDDS |
| 710 | 720 | 730 | 740 | 750 |
| DIVKKEFASI | LGQLVCTLHG | MFYLTSSLTE | PFSEHGHVDL | FCRNLKATSQ |
| 760 | 770 | 780 | 790 | 800 |
| HECSSSQLKA | SVCKPFLFLL | KKKIPSPVKL | AFIDNLHHLC | KHLDFREDET |
| 810 | 820 | 830 | 840 | 850 |
| DVKAVLGTLL | NLMEDPDKDV | RVAFSGNIKH | ILESLDSEDG | FIKELFVLRM |
| 860 | 870 | 880 | 890 | 900 |
| KEAYTHAQIS | RNNELKDTLI | LTTGDIGRAA | KGDLVPFALL | HLLHCLLSKS |
| 910 | 920 | 930 | 940 | 950 |
| ASVSGAAYTE | IRALVAAKSV | KLQSFFSQYK | KPICQFLVES | LHSSQMTALP |
| 960 | 970 | 980 | 990 | 1000 |
| NTPCQNADVR | KQDVAHQREM | ALNTLSEIAN | VFDFPDLNRF | LTRTLQVLLP |
| 1010 | 1020 | 1030 | 1040 | 1050 |
| DLAAKASPAA | SALIRTLGKQ | LNVNRREILI | NNFKYIFSHL | VCSCSKDELE |
| 1060 | 1070 | 1080 | 1090 | 1100 |
| RALHYLKNET | EIELGSLLRQ | DFQGLHNELL | LRIGEHYQQV | FNGLSILASF |
| 1110 | 1120 | 1130 | 1140 | 1150 |
| ASSDDPYQGP | RDIISPELMA | DYLQPKLLGI | LAFFNMQLLS | SSVGIEDKKM |
| 1160 | 1170 | 1180 | 1190 | 1200 |
| ALNSLMSLMK | LMGPKHVSSV | RVKMMTTLRT | GLRFKDDFPE | LCCRAWDCFV |
| 1210 | 1220 | 1230 | 1240 | 1250 |
| RCLDHACLGS | LLSHVIVALL | PLIHIQPKET | AAIFHYLIIE | NRDAVQDFLH |
| 1260 | 1270 | 1280 | 1290 | 1300 |
| EIYFLPDHPE | LKKIKAVLQE | YRKETSESTD | LQTTLQLSMK | AIQHENVDVR |
| 1310 | 1320 | 1330 | 1340 | 1350 |
| IHALTSLKET | LYKNQEKLIK | YATDSETVEP | IISQLVTVLL | KGCQDANSQA |
| 1360 | 1370 | 1380 | 1390 | 1400 |
| RLLCGECLGE | LGAIDPGRLD | FSTTETQGKD | FTFVTGVEDS | SFAYGLLMEL |
| 1410 | 1420 | 1430 | 1440 | 1450 |
| TRAYLAYADN | SRAQDSAAYA | IQELLSIYDC | REMETNGPGH | QLWRRFPEHV |
| 1460 | 1470 | 1480 | 1490 | 1500 |
| REILEPHLNT | RYKSSQKSTD | WSGVKKPIYL | SKLGSNFAEW | SASWAGYLIT |
| 1510 | 1520 | 1530 | 1540 | 1550 |
| KVRHDLASKI | FTCCSIMMKH | DFKVTIYLLP | HILVYVLLGC | NQEDQQEVYA |
| 1560 | 1570 | 1580 | 1590 | 1600 |
| EIMAVLKHDD | QHTINTQDIA | SDLCQLSTQT | VFSMLDHLTQ | WARHKFQALK |
| 1610 | 1620 | 1630 | 1640 | 1650 |
| AEKCPHSKSN | RNKVDSMVST | VDYEDYQSVT | RFLDLIPQDT | LAVASFRSKA |
| 1660 | 1670 | 1680 | 1690 | 1700 |
| YTRAVMHFES | FITEKKQNIQ | EHLGFLQKLY | AAMHEPDGVA | GVSAIRKAEP |
| 1710 | 1720 | 1730 | 1740 | 1750 |
| SLKEQILEHE | SLGLLRDATA | CYDRAIQLEP | DQIIHYHGVV | KSMLGLGQLS |
| 1760 | 1770 | 1780 | 1790 | 1800 |
| TVITQVNGVH | ANRSEWTDEL | NTYRVEAAWK | LSQWDLVENY | LAADGKSTTW |
| 1810 | 1820 | 1830 | 1840 | 1850 |
| SVRLGQLLLS | AKKRDITAFY | DSLKLVRAEQ | IVPLSAASFE | RGSYQRGYEY |
| 1860 | 1870 | 1880 | 1890 | 1900 |
| IVRLHMLCEL | EHSIKPLFQH | SPGDSSQEDS | LNWVARLEMT | QNSYRAKEPI |
| 1910 | 1920 | 1930 | 1940 | 1950 |
| LALRRALLSL | NKRPDYNEMV | GECWLQSARV | ARKAGHHQTA | YNALLNAGES |
| 1960 | 1970 | 1980 | 1990 | 2000 |
| RLAELYVERA | KWLWSKGDVH | QALIVLQKGV | ELCFPENETP | PEGKNMLIHG |
| 2010 | 2020 | 2030 | 2040 | 2050 |
| RAMLLVGRFM | EETANFESNA | IMKKYKDVTA | CLPEWEDGHF | YLAKYYDKLM |
| 2060 | 2070 | 2080 | 2090 | 2100 |
| PMVTDNKMEK | QGDLIRYIVL | HFGRSLQYGN | QFIYQSMPRM | LTLWLDYGTK |
| 2110 | 2120 | 2130 | 2140 | 2150 |
| AYEWEKAGRS | DRVQMRNDLG | KINKVITEHT | NYLAPYQFLT | AFSQLISRIC |
| 2160 | 2170 | 2180 | 2190 | 2200 |
| HSHDEVFVVL | MEIIAKVFLA | YPQQAMWMMT | AVSKSSYPMR | VNRCKEILNK |
| 2210 | 2220 | 2230 | 2240 | 2250 |
| AIHMKKSLEK | FVGDATRLTD | KLLELCNKPV | DGSSSTLSMS | THFKMLKKLV |
| 2260 | 2270 | 2280 | 2290 | 2300 |
| EEATFSEILI | PLQSVMIPTL | PSILGTHANH | ASHEPFPGHW | AYIAGFDDMV |
| 2310 | 2320 | 2330 | 2340 | 2350 |
| EILASLQKPK | KISLKGSDGK | FYIMMCKPKD | DLRKDCRLME | FNSLINKCLR |
| 2360 | 2370 | 2380 | 2390 | 2400 |
| KDAESRRREL | HIRTYAVIPL | NDECGIIEWV | NNTAGLRPIL | TKLYKEKGVY |
| 2410 | 2420 | 2430 | 2440 | 2450 |
| MTGKELRQCM | LPKSAALSEK | LKVFREFLLP | RHPPIFHEWF | LRTFPDPTSW |
| 2460 | 2470 | 2480 | 2490 | 2500 |
| YSSRSAYCRS | TAVMSMVGYI | LGLGDRHGEN | ILFDSLTGEC | VHVDFNCLFN |
| 2510 | 2520 | 2530 | 2540 | 2550 |
| KGETFEVPEI | VPFRLTHNMV | NGMGPMGTEG | LFRRACEVTM | RLMRDQREPL |
| 2560 | 2570 | 2580 | 2590 | 2600 |
| MSVLKTFLHD | PLVEWSKPVK | GHSKAPLNET | GEVVNEKAKT | HVLDIEQRLQ |
| 2610 | 2620 | 2630 | 2640 | 2644 |
| GVIKTRNRVT | GLPLSIEGHV | HYLIQEATDE | NLLCQMYLGW | TPYM |
Data source: UniProt
Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs
Uniprot Database Entry PhosphoSitePlus ®
Click a point on a node
to view detailed assay information below
All other points link out to UniProt
loading
Assay Details for CPTAC-3214 Collapse assay details
Data source: Panorama
-
Official Gene Symbol
- ATR Peptide Modified Sequence
- GVELC[+57.0]FPENETPPEGK Modification Type
- Carbamidomethyl Cysteine Protein - Site of Modification
- 1983 Peptide - Site of Modification
- 5 Peptide Start
- 1979 Peptide End
- 1994
-
CPTAC ID
- CPTAC-3214 Peptide Molecular Mass
- 1,801.8193 Species
- Homo sapiens (Human) Assay Type
- Enrichment ImmunoMRM Enrichment Method
- peptide immunoaffinity Matrix
- cell lysate Submitting Laboratory
- Fred Hutchinson Cancer Research Center Submitting Lab PI
- Amanda Paulovich
Publication
View Details (opens in a new window)
A Multiplexed Mass Spectrometry-Based Assay for Robust Quantification of Phosphosignaling in Response to DNA Damage. Whiteaker JR, Zhao L, Saul R, Kaczmarczyk JA, Schoenherr RM, Moore HD, Jones-Weinert C, Ivey RG, Lin C, Hiltke T, Reding KW, Whiteley G, Wang P, Paulovich AG. Radiat Res. 2018 Feb 23. doi: 10.1667/RR14963.1. [Epub ahead of print]
Assay Parameters Collapse assay parameters
Data source: Panorama
-
Instrument
- 6500 QTRAP (Sciex) Internal Standard
- synthetic peptide Peptide Standard Purity
- >95% Peptide Standard Label Type
- 13C and 15N at C-terminus K
-
LC
- Ultra NanoLC 2D+ (Eksigent) Column Packing
- ReproSil-Pur C18-AQ 3um Column Dimensions
- 100 x 0.075 mm Flow Rate
- 300 uL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y6 (1+) | 33.4 | 11.3 | 12.5 | 33.7 | 13.7 | 15.1 | 47.4 | 17.8 | 19.6 | 15 | 15 | 15 |
| y5 (1+) | 16.6 | 11.7 | 11.3 | 28.8 | 16.8 | 14.7 | 33.2 | 20.5 | 18.5 | 15 | 15 | 15 |
| y4 (1+) | 24.5 | 11 | 18.1 | 40.7 | 17.2 | 24 | 47.5 | 20.4 | 30.1 | 15 | 15 | 15 |
| y9 (1+) | 34.7 | 20.1 | 11.8 | 43.1 | 22.2 | 19.7 | 55.3 | 29.9 | 23 | 15 | 15 | 15 |
| y8 (1+) | 35.7 | 24.8 | 15.7 | 23.7 | 26.8 | 22.3 | 42.9 | 36.5 | 27.3 | 15 | 15 | 15 |
| y10 (1+) | 14.9 | 13.6 | 10.7 | 27.9 | 19.3 | 16.8 | 31.6 | 23.6 | 19.9 | 15 | 15 | 15 |
| sum | 11.2 | 6.9 | 9.9 | 22.4 | 13.8 | 14.7 | 25 | 15.4 | 17.7 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-3215 Collapse assay details
Data source: Panorama
-
Official Gene Symbol
- ATR Peptide Modified Sequence
- GVELC[+57.0]FPENET[+80.0]PPEGK Modification Type
- Carbamidomethyl Cysteine, Phospho (ST) Protein - Site of Modification
- 1983, 1989 Peptide - Site of Modification
- 5, 11 Peptide Start
- 1979 Peptide End
- 1994
-
CPTAC ID
- CPTAC-3215 Peptide Molecular Mass
- 1,881.7856 Species
- Homo sapiens (Human) Assay Type
- Enrichment ImmunoMRM Enrichment Method
- peptide immunoaffinity Matrix
- cell lysate Submitting Laboratory
- Fred Hutchinson Cancer Research Center Submitting Lab PI
- Amanda Paulovich
Publication
View Details (opens in a new window)
A Multiplexed Mass Spectrometry-Based Assay for Robust Quantification of Phosphosignaling in Response to DNA Damage. Whiteaker JR, Zhao L, Saul R, Kaczmarczyk JA, Schoenherr RM, Moore HD, Jones-Weinert C, Ivey RG, Lin C, Hiltke T, Reding KW, Whiteley G, Wang P, Paulovich AG. Radiat Res. 2018 Feb 23. doi: 10.1667/RR14963.1. [Epub ahead of print]
Assay Parameters Collapse assay parameters
Data source: Panorama
-
Instrument
- 6500 QTRAP (Sciex) Internal Standard
- synthetic peptide Peptide Standard Purity
- >95% Peptide Standard Label Type
- 13C and 15N at C-terminus K
-
LC
- Ultra NanoLC 2D+ (Eksigent) Column Packing
- ReproSil-Pur C18-AQ 3um Column Dimensions
- 100 x 0.075 mm Flow Rate
- 300 uL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y10 (1+) | 16 | 18.2 | 12.6 | 25.9 | 18.6 | 19.9 | 30.4 | 26 | 23.6 | 15 | 15 | 15 |
| y9 (1+) | 29.3 | 27.4 | 23 | 52.6 | 28.8 | 26.1 | 60.2 | 39.8 | 34.8 | 15 | 15 | 15 |
| y10-98 (1+) | 30.7 | 12.3 | 11.7 | 37.4 | 12.1 | 16.4 | 48.4 | 17.3 | 20.1 | 15 | 15 | 15 |
| y5 (1+) | 24.1 | 12.5 | 9.5 | 38 | 21 | 13.5 | 45 | 24.4 | 16.5 | 15 | 15 | 15 |
| sum | 11.5 | 11.9 | 8.8 | 26.1 | 16.4 | 14.1 | 28.5 | 20.3 | 16.6 | 15 | 15 | 15 |