CBR1, CPTAC-329 - CPTAC Assay Portal
- ️ABCC's Scientific Web Programming Group, Frederick National Laboratory for Cancer Research (FNLCR)
Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.
Protein Sequence hover to view complete sequence
| 10 | 20 | 30 | 40 | 50 |
| MSSGIHVALV | TGGNKGIGLA | IVRDLCRLFS | GDVVLTARDV | TRGQAAVQQL |
| 60 | 70 | 80 | 90 | 100 |
| QAEGLSPRFH | QLDIDDLQSI | RALRDFLRKE | YGGLDVLVNN | AGIAFKVADP |
| 110 | 120 | 130 | 140 | 150 |
| TPFHIQAEVT | MKTNFFGTRD | VCTELLPLIK | PQGRVVNVSS | IMSVRALKSC |
| 160 | 170 | 180 | 190 | 200 |
| SPELQQKFRS | ETITEEELVG | LMNKFVEDTK | KGVHQKEGWP | SSAYGVTKIG |
| 210 | 220 | 230 | 240 | 250 |
| VTVLSRIHAR | KLSEQRKGDK | ILLNACCPGW | VRTDMAGPKA | TKSPEEGAET |
| 260 | 270 | 277 | ||
| PVYLALLPPD | AEGPHGQFVS | EKRVEQW |
Data source: UniProt
Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs
Uniprot Database Entry PhosphoSitePlus ®
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to view detailed assay information below
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Assay Details for CPTAC-330 Collapse assay details
Data source: Panorama
-
Official Gene Symbol
- CBR1 Peptide Modified Sequence
- ILLNAC[+57.0]C[+57.0]PGWVR Modification Type
- Carbamidomethyl Cysteine, Carbamidomethyl Cysteine Protein - Site of Modification
- 226, 227 Peptide - Site of Modification
- 6, 7 Peptide Start
- 221 Peptide End
- 232
-
CPTAC ID
- CPTAC-330 Peptide Molecular Mass
- 1,457.7272 Species
- Homo sapiens (Human) Assay Type
- Direct MRM Matrix
- cell line lysate pool Submitting Laboratory
- Broad Institute and FHCRC Submitting Lab PI
- Steve Carr
Publication
View Details (opens in a new window)
Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253
Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.
Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.
Assay Parameters Collapse assay parameters
Data source: Panorama
-
Instrument
- 5500 QTRAP (ABSCIEX) Internal Standard
- peptide Peptide Standard Purity
- >95% Peptide Standard Label Type
- 13C,15N
-
LC
- nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent) Column Packing
- ChromXP C18-CL, 3 um, 120A Column Dimensions
- 150 x 0.075 mm Flow Rate
- 500 nL / min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y9 (1+) | 11.2 | 8.2 | 6.3 | 11.3 | 9.5 | 9.1 | 15.9 | 12.5 | 11.1 | 15 | 25 | 15 |
| y8 (1+) | 15 | 8.1 | 8.5 | 15.9 | 11.1 | 6.5 | 21.9 | 13.7 | 10.7 | 15 | 25 | 15 |
| y7 (1+) | 14.7 | 8.6 | 5.9 | 10.2 | 10.2 | 6.5 | 17.9 | 13.3 | 8.8 | 15 | 25 | 15 |
| sum | 9.9 | 6.7 | 5.7 | 8.3 | 9.2 | 5.1 | 12.9 | 11.4 | 7.6 | 15 | 25 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-329 Collapse assay details
Data source: Panorama
-
Official Gene Symbol
- CBR1 Peptide Sequence
- LFSGDVVLTAR Modification Type
- unmodified Protein - Site of Modification
- N/A Peptide - Site of Modification
- N/A Peptide Start
- 28 Peptide End
- 38
-
CPTAC ID
- CPTAC-329 Peptide Molecular Mass
- 1,176.6503 Species
- Homo sapiens (Human) Assay Type
- Direct MRM Matrix
- cell line lysate pool Submitting Laboratory
- Broad Institute and FHCRC Submitting Lab PI
- Steve Carr
Publication
View Details (opens in a new window)
Demonstrating the feasibility of large-scale development of standardized assays to quantify human proteins. Kennedy JJ, Abbatiello SE, Kim K, Yan P, Whiteaker JR, Lin C, Kim JS, Zhang Y, Wang X, Ivey RG, Zhao L, Min H, Lee Y, Yu MH, Yang EG, Lee C, Wang P, Rodriguez H, Kim Y, Carr SA, Paulovich AG. Nat Methods. 2014 Feb;11(2):149-55. doi: 10.1038/nmeth.2763. Epub 2013 Dec 8. PMID: 24317253
Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.
Response curves acquired at Broad Institute. Repeatability data acquired at Fred Hutchinson Cancer Research Center.
Assay Parameters Collapse assay parameters
Data source: Panorama
-
Instrument
- 5500 QTRAP (ABSCIEX) Internal Standard
- peptide Peptide Standard Purity
- >95% Peptide Standard Label Type
- 13C and 15N at C-terminus R
-
LC
- nanoLC-Ultra 2D, cHiPLC-nanoflex (Eksigent) Column Packing
- ChromXP C18-CL, 3 um, 120A Column Dimensions
- 150 x 0.075 mm Flow Rate
- 500 nL / min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y9 (1+) | 5.9 | 8 | 4.9 | 9.2 | 13.1 | 13.3 | 10.9 | 15.3 | 14.2 | 15 | 25 | 15 |
| y8 (1+) | 8.6 | 13.5 | 10.7 | 9 | 16.3 | 14.1 | 12.4 | 21.2 | 17.7 | 15 | 25 | 15 |
| y6 (1+) | 17.5 | 21.5 | 8.6 | 23.8 | 26.7 | 23.5 | 29.5 | 34.3 | 25 | 15 | 25 | 15 |
| sum | 6.7 | 8.4 | 5.3 | 9 | 13.4 | 13 | 11.2 | 15.8 | 14 | 15 | 25 | 15 |
Additional Resources and Comments
Assay Details for non-CPTAC-6083 Collapse assay details
Data source: Panorama
-
Official Gene Symbol
- CBR1 Peptide Sequence
- LFSGDVVLTAR Modification Type
- unmodified Protein - Site of Modification
- N/A Peptide - Site of Modification
- N/A Peptide Start
- 28 Peptide End
- 38
-
CPTAC ID
- non-CPTAC-6083 Peptide Molecular Mass
- 1,176.6503 Species
- Homo sapiens (Human) Assay Type
- Direct MRM Matrix
- Bovine Serum Albumin Submitting Laboratory
- McGill University Submitting Lab PI
- Christoph H. Borchers, Gerald Batist
Publication
View Details (opens in a new window)
mTORC1-driven protein translation correlates with clinical benefit of capivasertib within a genetically preselected cohort of PIK3CA-altered tumours. Constance A Sobsey, Bjoern C Froehlich, Georgia Mitsa, Sahar Ibrahim, Robert Popp, Rene P Zahedi, Elza C de Bruin, Christoph H Borchers, Gerald Batist
Assay Parameters Collapse assay parameters
Data source: Panorama
-
Instrument
- Agilent 6495B QQQ-MS Internal Standard
- Standard Isotope labeled internal standard peptides with 13Cx, 15Ny (R+10 or K+8) Peptide Standard Purity
- >95% Peptide Standard Label Type
- 13C and 15N at C-terminus R
-
LC
- Infinity 1290 Column Packing
- commercial (Agilent ZORBAX Eclipse Plus C18) Column Dimensions
- 4.6 mm × 150 mm, (5 µm) Flow Rate
- 0.4 mL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| b2 (1+) | 16.5 | 4 | 7.1 | 22.6 | 4.8 | 6.3 | 28 | 6.2 | 9.5 | 15 | 16 | 17 |
| y4 (1+) | 17.4 | 8 | 7.4 | 23.9 | 6 | 9.6 | 29.6 | 10 | 12.1 | 15 | 16 | 17 |
| y5 (1+) | 17.6 | 4.4 | 6.4 | 22 | 5.4 | 6.5 | 28.2 | 7 | 9.1 | 15 | 16 | 17 |
| y8 (1+) | 19.7 | 9.4 | 6.8 | 25.2 | 5.7 | 9.6 | 32 | 11 | 11.8 | 15 | 16 | 17 |
| y9 (1+) | 15.6 | 6.8 | 6.7 | 21.3 | 6.9 | 6.1 | 26.4 | 9.7 | 9.1 | 15 | 16 | 17 |
| sum | 13.6 | 3.9 | 3.7 | 18.2 | 4 | 3.2 | 22.7 | 5.6 | 4.9 | 15 | 16 | 17 |
Additional Resources and Comments
Assay Details for non-CPTAC-3775 Collapse assay details
Data source: Panorama
-
Official Gene Symbol
- Cbr1 Peptide Sequence
- FSGDVVLAAR Modification Type
- unmodified Protein - Site of Modification
- N/A Peptide - Site of Modification
- N/A Peptide Start
- 29 Peptide End
- 38
-
CPTAC ID
- non-CPTAC-3775 Peptide Molecular Mass
- 1,033.5556 Species
- Mus musculus (Mouse) Assay Type
- Direct MRM Matrix
- Plasma Submitting Laboratory
- UVic-Genome BC Proteomics Centre Submitting Lab PI
- Christoph Borchers
Assay Parameters Collapse assay parameters
Data source: Panorama
-
Instrument
- Agilent 6490/6495 QQQ Internal Standard
- synthetic peptide Peptide Standard Purity
- >80% Peptide Standard Label Type
- 13C and 15N at C-terminus R
-
LC
- 1290 LC (Agilent) Column Packing
- Zorbax Eclipse Plus C18, 1.8 µm Column Dimensions
- 2.1 x 150 mm Flow Rate
- 400 µL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| b2 (1+) | 8.4 | 8.1 | 8.9 | 8.8 | 13.3 | 12.8 | 12.2 | 15.6 | 15.6 | 15 | 15 | 15 |
| y4 (1+) | 8.7 | 4.4 | 5 | 10.4 | 6.3 | 8.1 | 13.6 | 7.7 | 9.5 | 15 | 15 | 15 |
| y5 (1+) | 8.8 | 4.3 | 3.7 | 10.6 | 8.2 | 7.5 | 13.8 | 9.3 | 8.4 | 15 | 15 | 15 |
| y8 (1+) | 7.8 | 3.4 | 6.2 | 12.7 | 7.1 | 7.9 | 14.9 | 7.9 | 10 | 15 | 15 | 15 |
| y9 (1+) | 9.6 | 5.4 | 4.7 | 10.2 | 9 | 8.8 | 14 | 10.5 | 10 | 15 | 15 | 15 |
| sum | 3.7 | 2 | 2.1 | 7.2 | 7.1 | 7.2 | 8.1 | 7.4 | 7.5 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for non-CPTAC-3416 Collapse assay details
Data source: Panorama
-
Official Gene Symbol
- Cbr1 Peptide Sequence
- TNFFGTR Modification Type
- unmodified Protein - Site of Modification
- N/A Peptide - Site of Modification
- N/A Peptide Start
- 113 Peptide End
- 119
-
CPTAC ID
- non-CPTAC-3416 Peptide Molecular Mass
- 841.4083 Species
- Mus musculus (Mouse) Assay Type
- Direct MRM Matrix
- Plasma Submitting Laboratory
- UVic-Genome BC Proteomics Centre Submitting Lab PI
- Christoph Borchers
Publication
View Details (opens in a new window)
Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.
Assay Parameters Collapse assay parameters
Data source: Panorama
-
Instrument
- Agilent 6490/6495 QQQ Internal Standard
- synthetic peptide Peptide Standard Purity
- >95% Peptide Standard Label Type
- 13C and 15N at C-terminus R
-
LC
- 1290 LC (Agilent) Column Packing
- Zorbax Eclipse Plus C18, 1.8 µm Column Dimensions
- 2.1 x 150 mm Flow Rate
- 400 µL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| b2 (1+) | 5.7 | 4.6 | 4.8 | 10.8 | 8.7 | 5 | 12.2 | 9.8 | 6.9 | 15 | 14 | 15 |
| y3 (1+) | 5.4 | 2.7 | 3.3 | 12.2 | 7.7 | 6.3 | 13.3 | 8.2 | 7.1 | 15 | 14 | 15 |
| y4 (1+) | 4 | 3.3 | 3.4 | 14.1 | 7.2 | 4.5 | 14.7 | 7.9 | 5.6 | 15 | 14 | 15 |
| y5 (1+) | 4 | 4.1 | 3.2 | 13.6 | 5.8 | 4.1 | 14.2 | 7.1 | 5.2 | 15 | 14 | 15 |
| y6 (1+) | 11.6 | 6.4 | 7.2 | 19.7 | 9.6 | 7.8 | 22.9 | 11.5 | 10.6 | 15 | 14 | 15 |
| sum | 1.7 | 1.5 | 1.3 | 12.8 | 6.2 | 3.2 | 12.9 | 6.4 | 3.5 | 15 | 14 | 15 |