IL16, CPTAC-926 - CPTAC Assay Portal
- ️ABCC's Scientific Web Programming Group, Frederick National Laboratory for Cancer Research (FNLCR)
Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.
Protein Sequence hover to view complete sequence
| 10 | 20 | 30 | 40 | 50 |
| MESHSRAGKS | RKSAKFRSIS | RSLMLCNAKT | SDDGSSPDEK | YPDPFEISLA |
| 60 | 70 | 80 | 90 | 100 |
| QGKEGIFHSS | VQLADTSEAG | PSSVPDLALA | SEAAQLQAAG | NDRGKTCRRI |
| 110 | 120 | 130 | 140 | 150 |
| FFMKESSTAS | SREKPGKLEA | QSSNFLFPKA | CHQRARSNST | SVNPYCTREI |
| 160 | 170 | 180 | 190 | 200 |
| DFPMTKKSAA | PTDRQPYSLC | SNRKSLSQQL | DCPAGKAAGT | SRPTRSLSTA |
| 210 | 220 | 230 | 240 | 250 |
| QLVQPSGGLQ | ASVISNIVLM | KGQAKGLGFS | IVGGKDSIYG | PIGIYVKTIF |
| 260 | 270 | 280 | 290 | 300 |
| AGGAAAADGR | LQEGDEILEL | NGESMAGLTH | QDALQKFKQA | KKGLLTLTVR |
| 310 | 320 | 330 | 340 | 350 |
| TRLTAPPSLC | SHLSPPLCRS | LSSSTCITKD | SSSFALESPS | APISTAKPNY |
| 360 | 370 | 380 | 390 | 400 |
| RIMVEVSLQK | EAGVGLGIGL | CSVPYFQCIS | GIFVHTLSPG | SVAHLDGRLR |
| 410 | 420 | 430 | 440 | 450 |
| CGDEIVEISD | SPVHCLTLNE | VYTILSHCDP | GPVPIIVSRH | PDPQVSEQQL |
| 460 | 470 | 480 | 490 | 500 |
| KEAVAQAVEN | TKFGKERHQW | SLEGVKRLES | SWHGRPTLEK | EREKNSAPPH |
| 510 | 520 | 530 | 540 | 550 |
| RRAQKVMIRS | SSDSSYMSGS | PGGSPGSGSA | EKPSSDVDIS | THSPSLPLAR |
| 560 | 570 | 580 | 590 | 600 |
| EPVVLSIASS | RLPQESPPLP | ESRDSHPPLR | LKKSFEILVR | KPMSSKPKPP |
| 610 | 620 | 630 | 640 | 650 |
| PRKYFKSDSD | PQKSLEEREN | SSCSSGHTPP | TCGQEARELL | PLLLPQEDTA |
| 660 | 670 | 680 | 690 | 700 |
| GRSPSASAGC | PGPGIGPQTK | SSTEGEPGWR | RASPVTQTSP | IKHPLLKRQA |
| 710 | 720 | 730 | 740 | 750 |
| RMDYSFDTTA | EDPWVRISDC | IKNLFSPIMS | ENHGHMPLQP | NASLNEEEGT |
| 760 | 770 | 780 | 790 | 800 |
| QGHPDGTPPK | LDTANGTPKV | YKSADSSTVK | KGPPVAPKPA | WFRQSLKGLR |
| 810 | 820 | 830 | 840 | 850 |
| NRASDPRGLP | DPALSTQPAP | ASREHLGSHI | RASSSSSSIR | QRISSFETFG |
| 860 | 870 | 880 | 890 | 900 |
| SSQLPDKGAQ | RLSLQPSSGE | AAKPLGKHEE | GRFSGLLGRG | AAPTLVPQQP |
| 910 | 920 | 930 | 940 | 950 |
| EQVLSSGSPA | ASEARDPGVS | ESPPPGRQPN | QKTLPPGPDP | LLRLLSTQAE |
| 960 | 970 | 980 | 990 | 1000 |
| ESQGPVLKMP | SQRARSFPLT | RSQSCETKLL | DEKTSKLYSI | SSQVSSAVMK |
| 1010 | 1020 | 1030 | 1040 | 1050 |
| SLLCLPSSIS | CAQTPCIPKE | GASPTSSSNE | DSAANGSAET | SALDTGFSLN |
| 1060 | 1070 | 1080 | 1090 | 1100 |
| LSELREYTEG | LTEAKEDDDG | DHSSLQSGQS | VISLLSSEEL | KKLIEEVKVL |
| 1110 | 1120 | 1130 | 1140 | 1150 |
| DEATLKQLDG | IHVTILHKEE | GAGLGFSLAG | GADLENKVIT | VHRVFPNGLA |
| 1160 | 1170 | 1180 | 1190 | 1200 |
| SQEGTIQKGN | EVLSINGKSL | KGTTHHDALA | ILRQAREPRQ | AVIVTRKLTP |
| 1210 | 1220 | 1230 | 1240 | 1250 |
| EAMPDLNSST | DSAASASAAS | DVSVESTAEA | TVCTVTLEKM | SAGLGFSLEG |
| 1260 | 1270 | 1280 | 1290 | 1300 |
| GKGSLHGDKP | LTINRIFKGA | ASEQSETVQP | GDEILQLGGT | AMQGLTRFEA |
| 1310 | 1320 | 1330 | 1332 | |
| WNIIKALPDG | PVTIVIRRKS | LQSKETTAAG | DS |
Data source: UniProt
Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs
Uniprot Database Entry PhosphoSitePlus ®
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to view detailed assay information below
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Assay Details for CPTAC-926 Collapse assay details
Data source: Panorama
-
Official Gene Symbol
- IL16 Peptide Modified Sequence
- LLS[+80.0]TQAEESQGPVLK Modification Type
- Phospho (ST) Protein - Site of Modification
- 946 Peptide - Site of Modification
- 3 Peptide Start
- 944 Peptide End
- 958
-
CPTAC ID
- CPTAC-926 Peptide Molecular Mass
- 1,678.8179 Species
- Homo sapiens (Human) Assay Type
- Enrichment MRM Enrichment Method
- IMAC Matrix
- cell lysate Submitting Laboratory
- Fred Hutchinson Cancer Research Center Submitting Lab PI
- Amanda Paulovich
Publication
View Details (opens in a new window)
Kennedy JJ, Yan P, Zhao L, Ivey RG, Voytovich U, Moore HD, Lin C, Pogosova-Agadjanyan EL, Stirewalt DL, Reding KW, Whiteaker JR, Paulovich AG. Immobilized metal affinity chromatography coupled to multiple reaction monitoring enables reproducible quantification of phospho-signaling. Molecular and Cellular Proteomics. mcp.O115.054940
Assay Parameters Collapse assay parameters
Data source: Panorama
-
Instrument
- QTrap 5500 (Sciex) Internal Standard
- Peptide Peptide Standard Purity
- >95% Peptide Standard Label Type
- 13C and 15N at C-terminus K
-
LC
- nanoLC-2D (Eksigent) Column Packing
- ChromXP C18-CL, 3 µm, 120 Å Column Dimensions
- 75 µm x 15cm Flow Rate
- 300 nL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y4 (1+) | 36.4 | 23.1 | 27.7 | 58.1 | 31.8 | 32.3 | 68.6 | 39.3 | 42.6 | 15 | 24 | 15 |
| y5 (1+) | 36.9 | 18.5 | 20.1 | 29.9 | 19.1 | 21.5 | 47.5 | 26.6 | 29.4 | 15 | 24 | 15 |
| y7 (1+) | 55.3 | 13.5 | 13.2 | 87.3 | 20 | 24.9 | 103.3 | 24.1 | 28.2 | 15 | 24 | 15 |
| sum | 36.5 | 11.3 | 11.2 | 55.7 | 14.9 | 14.4 | 66.6 | 18.7 | 18.2 | 15 | 24 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-6050 Collapse assay details
Data source: Panorama
-
Official Gene Symbol
- IL16 Peptide Sequence
- LLSTQAEESQGPVLK Modification Type
- unmodified Protein - Site of Modification
- N/A Peptide - Site of Modification
- N/A Peptide Start
- 944 Peptide End
- 958
-
CPTAC ID
- CPTAC-6050 Peptide Molecular Mass
- 1,598.8516 Species
- Human Assay Type
- Enrichment MRM Enrichment Method
- IMAC Matrix
- FFPE lung tumor tissue lysate pool Submitting Laboratory
- Fred Hutchinson Cancer Research Center Submitting Lab PI
- Amanda Paulovich
Publication
View Details (opens in a new window)
Whiteaker JR, Zhao L, Schoenherr RM, Huang D, Lundeen RA, Voytovich U, Kennedy JJ, Ivey RG, Lin C, Murillo OD, Lorentzen TD, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Reading JJ, Perry CD, Richardson CW, Garcia-Buntley SS, Bocik W, Hewitt SM, Chowdhury S, Vandermeer J, Smith SD, Gopal AK, Ramchurren N, Fling SP, Wang P, Paulovich AG. A multiplexed assay for quantifying immunomodulatory proteins supports correlative studies in immunotherapy clinical trials. Front Oncol. 2023 May 2;13:1168710. doi: 10.3389/fonc.2023.1168710. PMID: 37205196; PMCID: PMC10185886.
Assay Parameters Collapse assay parameters
Data source: Panorama
-
Instrument
- QTRAP 5500 Internal Standard
- peptide Peptide Standard Purity
- >95% Peptide Standard Label Type
- 13C and 15N at C-terminus K
-
LC
- Eksigent nanoLC 425 Column Packing
- 3 µm ChromXP C18EP, 120 Å Column Dimensions
- 150 x 0.075 mm Flow Rate
- 0.3 µL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| y10 (1+) | 23.9 | 15.7 | 19.9 | 22.3 | 16 | 20.6 | 32.7 | 22.4 | 28.6 | 15 | 15 | 15 |
| y11 (1+) | 36.7 | 15.3 | 31.5 | 44.9 | 15.1 | 32.5 | 58 | 21.5 | 45.3 | 15 | 15 | 15 |
| y4 (1+) | 30.4 | 11.3 | 19.1 | 30.3 | 11.5 | 24.4 | 42.9 | 16.1 | 31 | 15 | 15 | 15 |
| y5 (1+) | 23.3 | 10.8 | 24 | 22.1 | 11.2 | 24.9 | 32.1 | 15.6 | 34.6 | 15 | 15 | 15 |
| y7 (1+) | 28.9 | 20.8 | 22.8 | 30 | 23.7 | 21.9 | 41.7 | 31.5 | 31.6 | 15 | 15 | 15 |
| y8 (1+) | 23.7 | 10.7 | 20.8 | 24.6 | 10.6 | 19.2 | 34.2 | 15.1 | 28.3 | 15 | 15 | 15 |
| y9 (1+) | 18.6 | 10.6 | 21.9 | 25.7 | 13.5 | 22.6 | 31.7 | 17.2 | 31.5 | 15 | 15 | 15 |
| sum | 20.8 | 7.7 | 19.2 | 19.7 | 8.5 | 20 | 28.6 | 11.5 | 27.7 | 15 | 15 | 15 |