Mpo, CPTAC-3375 - CPTAC Assay Portal
- ️ABCC's Scientific Web Programming Group, Frederick National Laboratory for Cancer Research (FNLCR)
Please include the following statement when referencing the CPTAC Assay Portal
We would like to acknowledge the National Cancer Institute’s Clinical Proteomic Tumor Analysis Consortium (CPTAC) Assay Portal (assays.cancer.gov) for developing assays and establishing criteria for the assays described in this publication.
Protein Sequence hover to view complete sequence
| 10 | 20 | 30 | 40 | 50 |
| MKLLLALAGL | LAPLAMLQTS | NGATPALLGE | VENSVVLSCM | EEAKQLVDRA |
| 60 | 70 | 80 | 90 | 100 |
| YKERRESIKR | SLQSGSASPT | ELLFYFKQPV | AGTRTAVRAA | DYLHVALDLL |
| 110 | 120 | 130 | 140 | 150 |
| KRKLQPLWPR | PFNVTDVLTP | AQLNLLSVSS | GCAYQDVRVT | CPPNDKYRTI |
| 160 | 170 | 180 | 190 | 200 |
| TGHCNNRRSP | TLGASNRAFV | RWLPAEYEDG | VSMPFGWTPG | VNRNGFKVPL |
| 210 | 220 | 230 | 240 | 250 |
| ARQVSNAIVR | FPNDQLTKDQ | ERALMFMQWG | QFLDHDITLT | PEPATRFSFF |
| 260 | 270 | 280 | 290 | 300 |
| TGLNCETSCL | QQPPCFPLKI | PPNDPRIKNQ | KDCIPFFRSC | PACTRNNITI |
| 310 | 320 | 330 | 340 | 350 |
| RNQINALTSF | VDASGVYGSE | DPLARKLRNL | TNQLGLLAIN | TRFQDNGRAL |
| 360 | 370 | 380 | 390 | 400 |
| MPFDSLHDDP | CLLTNRSARI | PCFLAGDMRS | SEMPELTSMH | TLFVREHNRL |
| 410 | 420 | 430 | 440 | 450 |
| ATQLKRLNPR | WNGEKLYQEA | RKIVGAMVQI | ITYRDYLPLV | LGPAAMKKYL |
| 460 | 470 | 480 | 490 | 500 |
| PQYRSYNDSV | DPRIANVFTN | AFRYGHTLIQ | PFMFRLNNQY | RPTGPNPRVP |
| 510 | 520 | 530 | 540 | 550 |
| LSKVFFASWR | VVLEGGIDPI | LRGLMATPAK | LNRQNQIVVD | EIRERLFEQV |
| 560 | 570 | 580 | 590 | 600 |
| MRIGLDLPAL | NMQRSRDHGL | PGYNAWRRFC | GLPQPSTVGE | LGTVLKNLEL |
| 610 | 620 | 630 | 640 | 650 |
| ARKLMAQYGT | PNNIDIWMGG | VSEPLEPNGR | VGQLLACLIG | TQFRKLRDGD |
| 660 | 670 | 680 | 690 | 700 |
| RFWWENPGVF | SKQQRQALAS | ISLPRIICDN | TGITTVSKNN | IFMSNTYPRD |
| 710 | 718 | |||
| FVSCNTLPKL | NLTSWKET |
Data source: UniProt
Position of Targeted Peptide Analytes Relative to SNPs, Isoforms, and PTMs
Uniprot Database Entry PhosphoSitePlus ®
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Assay Details for CPTAC-6037 Collapse assay details
Data source: Panorama
-
Official Gene Symbol
- MPO Peptide Modified Sequence
- IGLDLPALNM[+15.994915]QR Modification Type
- Oxidation (M) Protein - Site of Modification
- 588 Peptide - Site of Modification
- 10 Peptide Start
- 579 Peptide End
- 590
-
CPTAC ID
- CPTAC-6037 Peptide Molecular Mass
- 1,355.7231 Species
- Human Assay Type
- Enrichment MRM Enrichment Method
- N/A Matrix
- FFPE lung tumor tissue lysate pool Submitting Laboratory
- Fred Hutchinson Cancer Research Center Submitting Lab PI
- Amanda Paulovich
Publication
View Details (opens in a new window)
Whiteaker JR, Zhao L, Schoenherr RM, Huang D, Lundeen RA, Voytovich U, Kennedy JJ, Ivey RG, Lin C, Murillo OD, Lorentzen TD, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Reading JJ, Perry CD, Richardson CW, Garcia-Buntley SS, Bocik W, Hewitt SM, Chowdhury S, Vandermeer J, Smith SD, Gopal AK, Ramchurren N, Fling SP, Wang P, Paulovich AG. A multiplexed assay for quantifying immunomodulatory proteins supports correlative studies in immunotherapy clinical trials. Front Oncol. 2023 May 2;13:1168710. doi: 10.3389/fonc.2023.1168710. PMID: 37205196; PMCID: PMC10185886.
Assay Parameters Collapse assay parameters
Data source: Panorama
-
Instrument
- QTRAP 5500 Internal Standard
- peptide Peptide Standard Purity
- >95% Peptide Standard Label Type
- 13C and 15N at C-terminus R
-
LC
- Eksigent nanoLC 425 Column Packing
- 3 µm ChromXP C18EP, 120 Å Column Dimensions
- 150 x 0.075 mm Flow Rate
- 0.3 µL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| b4 (1+) | 61 | 67.6 | 40.5 | 70.7 | 86.3 | 59.5 | 93.4 | 109.6 | 72 | 15 | 15 | 15 |
| b5 (1+) | 41.8 | 60.7 | 58.9 | 69.1 | 59.7 | 75.7 | 80.8 | 85.1 | 95.9 | 15 | 15 | 15 |
| y4 (1+) | 78.4 | 71.2 | 58.6 | 84.2 | 72.7 | 72.7 | 115 | 101.8 | 93.4 | 15 | 15 | 15 |
| y6 (1+) | 72.4 | 70.4 | 53.7 | 79.1 | 87.5 | 76.1 | 107.2 | 112.3 | 93.1 | 15 | 15 | 15 |
| y7 (1+) | 42.9 | 62.4 | 33.3 | 42.4 | 74.9 | 40.6 | 60.3 | 97.5 | 52.5 | 15 | 15 | 15 |
| y8 (1+) | 48.6 | 68.8 | 31.2 | 45.1 | 67.7 | 74 | 66.3 | 96.5 | 80.3 | 15 | 15 | 15 |
| y9 (1+) | 54 | 85.5 | 44.5 | 56.4 | 83.4 | 64.3 | 78.1 | 119.4 | 78.2 | 15 | 15 | 15 |
| sum | 34.9 | 62 | 32.4 | 32 | 71.4 | 48.1 | 47.3 | 94.6 | 58 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for CPTAC-6053 Collapse assay details
Data source: Panorama
-
Official Gene Symbol
- MPO Peptide Sequence
- IGLDLPALNMQR Modification Type
- unmodified Protein - Site of Modification
- N/A Peptide - Site of Modification
- N/A Peptide Start
- 579 Peptide End
- 590
-
CPTAC ID
- CPTAC-6053 Peptide Molecular Mass
- 1,339.7282 Species
- Human Assay Type
- Enrichment MRM Enrichment Method
- N/A Matrix
- FFPE lung tumor tissue lysate pool Submitting Laboratory
- Fred Hutchinson Cancer Research Center Submitting Lab PI
- Amanda Paulovich
Publication
View Details (opens in a new window)
Whiteaker JR, Zhao L, Schoenherr RM, Huang D, Lundeen RA, Voytovich U, Kennedy JJ, Ivey RG, Lin C, Murillo OD, Lorentzen TD, Colantonio S, Caceres TW, Roberts RR, Knotts JG, Reading JJ, Perry CD, Richardson CW, Garcia-Buntley SS, Bocik W, Hewitt SM, Chowdhury S, Vandermeer J, Smith SD, Gopal AK, Ramchurren N, Fling SP, Wang P, Paulovich AG. A multiplexed assay for quantifying immunomodulatory proteins supports correlative studies in immunotherapy clinical trials. Front Oncol. 2023 May 2;13:1168710. doi: 10.3389/fonc.2023.1168710. PMID: 37205196; PMCID: PMC10185886.
Assay Parameters Collapse assay parameters
Data source: Panorama
-
Instrument
- QTRAP 5500 Internal Standard
- peptide Peptide Standard Purity
- >95% Peptide Standard Label Type
- 13C and 15N at C-terminus R
-
LC
- Eksigent nanoLC 425 Column Packing
- 3 µm ChromXP C18EP, 120 Å Column Dimensions
- 150 x 0.075 mm Flow Rate
- 0.3 µL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| b5 (1+) | 20.5 | 20.8 | 19.8 | 18.4 | 22.7 | 23.4 | 27.5 | 30.8 | 30.7 | 15 | 15 | 15 |
| y7 (1+) | 11 | 24.3 | 17.1 | 12.2 | 24.3 | 17.9 | 16.4 | 34.4 | 24.8 | 15 | 15 | 15 |
| y8 (1+) | 9.3 | 22.3 | 20.4 | 16.2 | 26 | 27.3 | 18.7 | 34.3 | 34.1 | 15 | 15 | 15 |
| y9 (1+) | 17.9 | 24.9 | 24.6 | 18.4 | 29 | 25.9 | 25.7 | 38.2 | 35.7 | 15 | 15 | 15 |
| sum | 10.7 | 23.8 | 16.9 | 12 | 24.1 | 18 | 16.1 | 33.9 | 24.7 | 15 | 15 | 15 |
Additional Resources and Comments
Assay Details for non-CPTAC-3375 Collapse assay details
Data source: Panorama
-
Official Gene Symbol
- Mpo Peptide Sequence
- IANVFTNAFR Modification Type
- unmodified Protein - Site of Modification
- N/A Peptide - Site of Modification
- N/A Peptide Start
- 464 Peptide End
- 473
-
CPTAC ID
- non-CPTAC-3375 Peptide Molecular Mass
- 1,151.6087 Species
- Mus musculus (Mouse) Assay Type
- Direct MRM Matrix
- Plasma Submitting Laboratory
- UVic-Genome BC Proteomics Centre Submitting Lab PI
- Christoph Borchers
Publication
View Details (opens in a new window)
Molecular phenotyping of laboratory mouse strains using 500 multiple reaction monitoring mass spectrometry plasma assays. Michaud SA, Sinclair NJ, Petrošová H, Palmer AL, Pistawka AJ, Zhang S, Hardie DB, Mohammed Y Eshghi A1, Richard VR, Sickmann A, Borchers CH. Commun Biol. 2018 Jun 27;1:78. doi: 10.1038/s42003-018-0087-6. eCollection 2018.
Assay Parameters Collapse assay parameters
Data source: Panorama
-
Instrument
- Agilent 6490/6495 QQQ Internal Standard
- synthetic peptide Peptide Standard Purity
- >95% Peptide Standard Label Type
- 13C and 15N at C-terminus R
-
LC
- 1290 LC (Agilent) Column Packing
- Zorbax Eclipse Plus C18, 1.8 µm Column Dimensions
- 2.1 x 150 mm Flow Rate
- 400 µL/min
Chromatograms
Data source: Panorama
Response Curves
Data source: Panorama
Retrieving Data
Repeatability
Data source: Panorama
| Average intra-assay CV (within day CV) |
Average inter-assay CV (between day CV) |
Total CV |
n= | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fragment ion / Transition | Low | Med | High | Low | Med | High | Low | Med | High | Low | Med | High |
| b4 (1+) | 11.9 | 6.6 | 3.9 | 19.1 | 10.3 | 4.9 | 22.5 | 12.2 | 6.3 | 15 | 15 | 15 |
| b3 (1+) | 11.7 | 4 | 5.8 | 26.9 | 12.8 | 7.1 | 29.3 | 13.4 | 9.2 | 15 | 15 | 15 |
| y5 (1+) | 3.5 | 6.5 | 6 | 13.7 | 11.3 | 6.8 | 14.1 | 13 | 9.1 | 15 | 15 | 15 |
| y6 (1+) | 5.8 | 6.7 | 3.6 | 13.6 | 11.7 | 4.7 | 14.8 | 13.5 | 5.9 | 15 | 15 | 15 |
| y8 (1+) | 5.5 | 5.2 | 5.7 | 13.3 | 7.9 | 6.2 | 14.4 | 9.5 | 8.4 | 15 | 15 | 15 |
| sum | 2.6 | 3 | 1.5 | 14.4 | 9.5 | 3.8 | 14.6 | 10 | 4.1 | 15 | 15 | 15 |