cDNA-AFLP analysis reveals differential gene expression in compatible interaction of wheat challenged with Puccinia striiformis f. sp. tritici - BMC Genomics

Quantitative real-time PCR (qRT-PCR) analyses of 29 selected genes. Leaf tissues were sampled for both inoculated and mock-inoculated plants at 12, 18, 24, 48, 72 and 120 hpi, as well as mock-inoculated plants near 0 hpi. Three independent biological replications were performed. The relative expression level for Pst-inoculated plants at each time point was calculated as fold of the mock-inoculated plants at that time point using the comparative ΔΔCT method. All data were normalized to the 18S rRNA expression level. The mean expression value was calculated for every transcript derived fragment (TDF) with three replications.

Back to article page