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Polyclonal Antibodies Against the Ultrafast Multisubstrate Deoxyribonucleoside Kinase from Drosophila Melanogaster

Conclusion

Histidine tagged recombinant Dm-dNK was shown to be superior to untagged protein as antigen for generating antibodies against Dm-dNK. The antiserum to rDm-dNK-8His was able to recognise recombinant Dm-dNK expressed in different hosts and will therefore be a valuable tool to investigate subcellular localization and expression levels of Dm-dNK in homologous and heterologous expression.

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Author information

Authors and Affiliations

  1. Department of Microbiology, Technical University of Denmark, DK-2800, Lyngby

    Wolfgang Knecht & Jure Piskur

  2. Department of Life Sciences and Chemistry, Roskilde University, DK-4000, Roskilde

    Birgitte Munch-Petersen

Authors

  1. Wolfgang Knecht

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  2. Birgitte Munch-Petersen

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  3. Jure Piskur

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Editor information

Editors and Affiliations

  1. Tel-Aviv University, Tel-Aviv, Israel

    Esther Zoref-Shani  & Oded Sperling  & 

  2. Rabin Medical Center, Petah-Tikva, Israel

    Oded Sperling

© 2002 Kluwer Academic Publishers

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Knecht, W., Munch-Petersen, B., Piskur, J. (2002). Polyclonal Antibodies Against the Ultrafast Multisubstrate Deoxyribonucleoside Kinase from Drosophila Melanogaster. In: Zoref-Shani, E., Sperling, O. (eds) Purine and Pyrimidine Metabolism in Man X. Advances in Experimental Medicine and Biology, vol 486. Springer, Boston, MA. https://doi.org/10.1007/0-306-46843-3_51

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  • DOI: https://doi.org/10.1007/0-306-46843-3_51

  • Publisher Name: Springer, Boston, MA

  • Print ISBN: 978-0-306-46515-4

  • Online ISBN: 978-0-306-46843-8

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