Microdissected Domes from Gut-Associated Lymphoid Tissues: A Model of M Cell Transepithelial Transport In Vitro
Abstract
Specialized M cells in gut-associated lymphoid tissues (GALT) comprise 10-50% of the epithelial cell population overlying GALT domes,1,2 and exhibit short microvilli, numerous apical vesicles, and a basolateral pocket infiltrated by clusters of lymphoid cells.3,4 Functionally, M cells endocytose protein molecules,5 viruses,6 and Gram-microorganisms.7 Particle binding to the M cell apical membrane results in rapid (2 µm/min) internalization and shuttling to pocket domains and to underlying dome lymphoid tissue.8 While it has been shown that lymphocytes which infiltrate M cell pockets and localize to subepithelial GALT domes are MHC class II+ activated T cells,4,9 and that mucosal immunization with structures which are taken up by M cells into GALT may result in the development of IgA responses,10 the relationship between M cell-mediated antigen uptake and the evolution of IgA antibody has not been examined directly. A model using microdissected domes from GALT was developed to enable the in vitro screening of a large repertoire of antigens for M cell tropism, and to study the development of IgA antibody responses to antigens recognized by M cells.
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Vaccine Delivery Research, OraVax Inc., Cambridge, MA, 02139, USA
Zita Kabok, Thomas H. Ermak & Jacques Pappo
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- Zita Kabok
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- Thomas H. Ermak
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- Jacques Pappo
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University of Alabama at Birmingham, Birmingham, Alabama, USA
Jiri Mestecky , Michael W. Russell , Susan Jackson & Suzanne M. Michalek , , &
Academy of Sciences of the Czech Republic, Prague, Czech Republic
Helena Tlaskalová-Hogenová & Jaroslav Šterzl &
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© 1995 Springer Science+Business Media New York
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Kabok, Z., Ermak, T.H., Pappo, J. (1995). Microdissected Domes from Gut-Associated Lymphoid Tissues: A Model of M Cell Transepithelial Transport In Vitro . In: Mestecky, J., Russell, M.W., Jackson, S., Michalek, S.M., Tlaskalová-Hogenová, H., Šterzl, J. (eds) Advances in Mucosal Immunology. Advances in Experimental Medicine and Biology, vol 371. Springer, Boston, MA. https://doi.org/10.1007/978-1-4615-1941-6_49
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DOI: https://doi.org/10.1007/978-1-4615-1941-6_49
Publisher Name: Springer, Boston, MA
Print ISBN: 978-1-4613-5796-4
Online ISBN: 978-1-4615-1941-6
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