Immunohistochemical co-localization of glycogen phosphorylase with the astroglial markers glial fibrillary acidic protein and S-100 protein in rat brain sections - Histochemistry and Cell Biology
- ️Hamprecht, B.
- ️Mon Jun 01 1992
Summary
Immunofluorescence double-labelling and immunoenzyme double-staining methods were used to examine the location of glycogen phosphorylase brain isozyme with the astrocyte markers glial fibrillary acidic protein (GFAP) and S-100 protein in formaldehydefixed, paraffin-embedded slices from adult rat brain. Astrocytes in the cerebellum and the hippocampus, which express GFAP or S-100 protein immunoreactivity, show glycogen phosphorylase immunoreactivity. Regional intensity and intracellular distribution of the three antigens vary characteristically. In ependymal cells, glycogen phosphorylase immunoreactivity is co-localized with S-100 protein immunoreactivity, but not with GFAP immunoreactivity. These findings confirm that glycogen phosphorylase in the rat brain is exclusively localized in astrocytes and ependymal cells. All astrocytes, as far as they express GFAP or S-100 protein, do contain glycogen phosphorylase.
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References
Bignami A, Dahl D, Rueger DG (1980) Glial fibrillary acidic (GFA) protein in normal neural cells and pathological conditions. In: Fedoroff S, Hertz L (eds) Advances in cellular neurobiology, vol 1. Academic Press, New York, pp 285–310
Breisch M (1990) Isolierung, Kultivierung und Charakterisierung von Gefäßendothelzellen, insbesondere von Endothelzellen der Mikrokapillaren des cerebralen Cortex der Ratte. Thesis, University of Tübingen, Faculty of Biology
Chapman AG, Meldrum BS, Siesjö BK (1977) Cerebral metabolic changes during prolonged epileptic seizures in rats. J Neurochem 28:1025–1035
Cicero TJ, Cowan WM, Moore BW, Suntzeff V (1970) The cellular localization of the two brain-specific proteins, S-100 and 14-3-2. Brain Res 18:25–34
De Armond SJ, Eng LF, Rubinstein IJ (1980) The application of glial fibrillary acidic (GFA) protein immunohistochemistry in neurooncology: a progress report. Pathol Res Pract 168:374–394
De Vellis J, Wu DK, Kumar S (1986) Enzyme induction and regulation of protein synthesis. In: Fedoroff S, Vernadakis A (eds) Astrocytes. Development, morphology and regional specifications of astrocytes, vol 2. Academic Press, New York, pp 209–237
Duffy TE, Howse DC, Plum F (1975) Cerebral energy metabolism during experimental status epilepticus. J Neurochem 24:925–934
Eng LF, De Armond SJ (1983) Immunochemistry of the glial fibrillary acidic (GFA) protein. In: Zimmerman M (ed) Progress in neuropathology, vol 5. Raven Press, New York, pp 19–39
Eng LF, Gerstel B, Vanderhaeghen JJ (1970) A study of proteins in old multiple sclerosis plaques. Trans Am Soc Neurochem 1:42
Eng LF, Vanderhaeghen JJ, Bignami A, Gerstl B (1971) An acidic protein isolated from fibrous astrocytes. Brain Res 28:351–354
Folbergrova J (1975) Changes in glycogen phosphorylase activity and glycogen levels of mouse cerebral cortex during convulsions induced by homocysteine. J Neurochem 24:15–20
Ghandour MS, Labourdette G, Vincendon G, Gombos G (1981 a) A biochemical and immunohistological study of S-100 protein in developing rat cerebellum. Dev Neurosci 4:98–109
Ghandour MS, Langley OK, Labourdette G, Vincendon G, Gombos G (1981 b) Specific and artefactual cellular localization of S-100 protein: an astrocyte marker in rat cerebellum. Dev Neurosci 4:66–78
Ignacio PC, Baldwin BA, Vijayan VK, Tait RC, Gorin FA (1990) Brain isozyme of glycogen phosphorylase: immunohistological localization within the central nervous system. Brain Res 529:42–49
Kato K, Shimizu A, Kurobe N, Takashi M, Koshikawa T (1989) Human brain-type glycogen phosphorylase: quantitative localization in human tissues determined with an immunoassay system. J Neurochem 52:1425–1432
Khalfan H, Abaknesha R, Rand-Weaver M, Rice RG, Robinson D (1986) Aminomethyl coumarin acetic acid: a new fluorescent labeling agent for proteins. Histochem J 18:497–499
Ludwin SK, Kosek JC, Eng LF (1976) The topographical distribution of S-100 and GFA proteins in the adult rat brain: An immunohistochemical study using horseradish peroxidase-labeled antibodies. J Comp Neurol 165:197–200
Matus A, Mughal S (1975) Immunohistochemical localization of S-100 protein in brain. Nature 258:746–748
Moore BW (1965) A soluble protein characteristic of the nervous system. Biochem Biophys Res Commun 19:739–744
Newgard CB, Hwang PK, Fletterick RJ (1989) The family of glycogen phosphorylases: structure and function. Crit Rev Biochem Mol Biol 24:69–99
Norenberg MD (1979) The distribution of glutamine synthetase in the rat central nervous system. J Histochem Cytochem 27:756–762
Norenberg MD, Martinez-Hernandez A (1979) Fine structural localization of glutamine synthetase in astrocytes of rat brain. Brain Res 161:303–310
Palay SL, Chan-Palay V (1974) Cerebellar cortex. Cytology and organization. Springer, Berlin Heidelberg New York, pp 288–320
Pfeiffer B, Elmer K, Roggendorf W, Reinhart PH, Hamprecht B (1990) Immunohistochemical demonstration of glycogen phosphorylase in rat brain slices. Histochemistry 94:73–80
Reinhart PH, Pfeiffer B, Spengler S, Hamprecht B (1990) Purification of glycogen phosphorylase from bovine brain and immunocytochemical examination of rat glial primary cultures using monoclonal antibodies raised against this enzyme. J Neurochem 54:1474–1483
Vandesande F (1983) Immunohistochemical double staining techniques. In: Cuello AC (ed) Immunohistochemistry, IBRO handbook, Wiley, Chichester, pp 257–272
Watanabe H, Passonneau JV (1973) Factors affecting the turnover of cerebral glycogen and limit dextrin in vivo. J Neurochem 20:1543–1554
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Physiologisch-Chemisches Institut, University of Tübingen, Hoppe-Seyler-Strasse 4, W-7400, Tübingen, Federal Republic of Germany
B. Pfeiffer & B. Hamprecht
Institut für Hirnforschung, University of Tübingen, Calwer Strasse 3, W-7400, Tübingen, Federal Republic of Germany
R. Meyermann
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Pfeiffer, B., Meyermann, R. & Hamprecht, B. Immunohistochemical co-localization of glycogen phosphorylase with the astroglial markers glial fibrillary acidic protein and S-100 protein in rat brain sections. Histochemistry 97, 405–412 (1992). https://doi.org/10.1007/BF00270387
Accepted: 07 April 1992
Issue Date: June 1992
DOI: https://doi.org/10.1007/BF00270387