The heart-gill preparation of the eel and its perfusion for the study of a natural membrane in situ - Journal of Comparative Physiology A
- ️Keys, Ancel B.
- ️Mon Jun 01 1931
The isolated heart-gill preparation of the eel is presented. Essentially it is a double perfusion system in which both internal and external mediums are circulated separately. The applicability of this preparation to other species of fish besides the eel is indicated.
The apparatus arrangement, operative technique, and the procedure for making the most carefully controlled studies of the permeability and chemical activity of the gills are described in detail.
The composition and preparation of various concentrations of a perfusion medium developed for use in this preparation are given.
It is shown that the preparation retains its vital properties for a period of hours and is physiologically acceptable.
The utilization of the preparation as a potent research tool in the investigation of the physiology 6f the gills and heart, as well as its use from the aspect of the properties of membranes, are discussed.