Dominant versus recessive behavior of a cold- and a heat-sensitive mammalian cell cycle variant in heterokaryons - Somatic Cell and Molecular Genetics
- ️Schindler, R.
- ️Tue Sep 01 1981
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Abstract
A heat-sensitive (hs, arrested at 39.5 °C, termed 21-Ta) and a cold-sensitive (cs, arrested at 33 °C, termed 21-Fb) clonal cell cycle variant were isolated from the same clone of the P-815 murine mastocytoma line. At the respective nonpermissive temperatures, both the hs and the cs variant were reversibly arrested in G1 phase, and numbers of cells forming colonies upon reincubation at the permissive temperature remained nearly constant for at least 6 days. Cells arrested in G1 by incubation at the respective nonpermissive temperatures were fused to cells of another P-815 clone (31-S) that had been arrested by serum deprivation. Upon reincubation in medium containing 10% serum for 48 h at 39.5° C, 21-Ta × 31-S heterokaryons, similar to 31-S × 31-S homokaryons, entered the S phase, whereas at 33°C, 21-Fb × 31-S heterokaryons, similar to 21-Fb × 21-Fb homokaryons, remained arrested in G1, indicating a recessive expression of the hs and a dominant expression of the cs phenotype.
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Authors and Affiliations
Department of Pathology, University of Bern, Freiburgstrasse 30, 3010, Bern, Switzerland
A. Zimmermann, J. C. Schaer, J. Schneider, P. Molo & R. Schindler
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- A. Zimmermann
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- J. C. Schaer
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- J. Schneider
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- P. Molo
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- R. Schindler
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Zimmermann, A., Schaer, J.C., Schneider, J. et al. Dominant versus recessive behavior of a cold- and a heat-sensitive mammalian cell cycle variant in heterokaryons. Somat Cell Mol Genet 7, 591–601 (1981). https://doi.org/10.1007/BF01549661
Received: 30 March 1981
Accepted: 04 June 1981
Issue Date: September 1981
DOI: https://doi.org/10.1007/BF01549661