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Loss and apoptosis of smooth muscle cells in intracranial aneurysms studies with in situ DNA end labeling and antibody against single-stranded DNA - Acta Neurochirurgica

  • ️Hayakawa, T.
  • ️Thu May 01 1997

Summary

Pathological specimens were collected from 14 unruptured and 13 ruptured aneurysms at the time of clipping and studied in order to assess the underlying mechanism of rupture by investigating degeneration of the aneurysmal wall and possible involvement of apoptosis. Immunohistochemistry with anti-actin antibody showed few smooth muscle cells in the ruptured aneurysms and replacement of the muscularis layer by a fibro-hyalin tissue. However, at least one layer of smooth muscle cells was clearly observed in the unruptured aneurysms. Thus, smooth muscle cells in the wall of the ruptured aneurysms were much more degenerated than those in the wall of unruptured aneurysms. In addition, unruptured aneurysms with an angiographically smooth wall showed well-layered positive staining for anti-smooth muscle actin antibody while those with irregular shapes rarely reacted. We found, for the first time, evidence of DNA fragmentation in the aneurysmal wall. Apoptotic bodies were detected by means of a terminal transferase (TdT)-mediated dUTP biotin nick end labelling technique (TUNEL) and an anti-single-stranded DNA antibody in 54% (7/13) of the ruptured aneurysms. In contrast, apoptotic bodies were found in only 7% (1/14) of the unruptured cases. These results suggest that apoptotic cell death might be involved in the rupture of aneurysms.

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References

  1. Bjorkerud S, Bjorkerud B, Joelsson M (1994) Structural organization of reconstituted human arterial smooth muscle tissue. Arterioscler Thromb 14(4): 644–651

    PubMed  Google Scholar 

  2. Gavrieli Y, Yoshida Y, Ben-Sasson S (1992) Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. J Cell Biol 119: 493–501

    PubMed  Google Scholar 

  3. Juvela S, Porras M, Heiskanen O (1993) Natural history of unruptured intracranial aneurysms: a long-term follow-up study. J Neurosurg 79: 174–182

    PubMed  Google Scholar 

  4. Keino H, Masaki S, Keyword S, Naruse I (1994) Apoptotic degeneration in the arhinencephalic brain of the mouse mutant Pdn/Pdn. Brain Res Dev Brain Res 78(2): 161–168

    PubMed  Google Scholar 

  5. Kerr J (1971) Shrinkage necrosis: a distinct mode of cellular death. J Pathol 105: 13–20

    PubMed  Google Scholar 

  6. Kitagawa Y, Okuhara E (1981) Anti-poly(I)-poly(C) antibody bound to cellulose and its use in the specific separation of double-stranded RNAs. Anal Biochem 115: 102–108

    PubMed  Google Scholar 

  7. Kockx MM, Cambier BA, Bortier HE, De Meyer G, Declercq SC, van Cauwelaert P (1994) Foam cell replication and smooth muscle cell apoptosis in human saphenous vein grafts. Histopathology 25(4): 365–371

    PubMed  Google Scholar 

  8. Leszczynski D, Zhao Y, Luokkamaki M, Foegh ML (1994) Apoptosis of vascular smooth muscle cells. Protein kinase C and oncoprotein Bcl-2 are involved in regulation of apoptosis in non-transformed rat vascular smooth muscle cells. Am J Pathol 145(6): 1265–1270

    PubMed  Google Scholar 

  9. Li Y, Chopp M, Jiang N, Yao F, Zaloge C (1995) Temporal profile of in situ DNA fragmentation after transient middle cerebral artery occlusion in the rat. J Cereb Blood Flow Metab 15: 389–397

    PubMed  Google Scholar 

  10. Naruse I, Keino H, Keyword Y (1994) Antibody against single-stranded DNA detects both programmed cell death and drug-induced apoptosis. Histochemistry 101(1): 73–78

    PubMed  Google Scholar 

  11. Plescia J (1964) Production of antibodies to denatured deoxyribonucleic acid. Proc Natl Acad Sci USA 52: 279–285

    PubMed  Google Scholar 

  12. Savill J (1994) Apoptosis in disease. Eur J Clin Invest 24: 715–723

    PubMed  Google Scholar 

  13. Schumer M, Colombel MC, Sawczuk IS, Gobe G, Connor J, O'Toole KM, Olsson CA, Wise GJ, Buttyan R (1992) Morphologic, biochemical and molecular evidence of apoptosis during the reperfusion phase after brief periods of renal ischemia. Am J Pathol 140: 831–838

    PubMed  Google Scholar 

  14. Taneda M, Yamada K, Kinoshita A, Hayakawa T, Kadota E (1992) Do incidental aneurysms really rupture in future? Jpn J Stroke 14: 768–772

    Google Scholar 

  15. Tomei L (1991) Apoptosis: a program for death or survival? Apoptosis. The Molecular Basis of Cell Death 279–316

  16. Tominaga T, Kure S, Narisawa K, Yoshimoto T (1993) Endonuclease activation following focal ischemic injury in the rat brain. Brain Res 608: 21–26

    PubMed  Google Scholar 

  17. Wijsman JH, Jonker RR, Keijzer R, Yen de Velde CJH, Cornelisse CJ, Van Dierendonck JH (1993) A new method to detect apoptosis in paraffin sections: in situ end-labeling of fragmented DNA. J Histochem Cytochem 41: 7–12

    PubMed  Google Scholar 

  18. Wood K, Dipasquale B, Youle R (1993) In situ labeling of granule cells for apoptosis-associated DNA fragmentation reveals different mechanisms of cell loss in developing cerebellum. Neuron 11: 621–632

    PubMed  Google Scholar 

  19. Yamada H, Hirai S, Ikegemi S, Keyword Y, Okuhara E, Nagano H (1985) The fate of DNA originally existing in the zygote nucleus during achromosomal cleavage of fertilized echinoderm eggs in the presence of aphidicolon: Microscopic studies with anti-DNA antibody. J Cell Physiol 124: 9–12

    PubMed  Google Scholar 

  20. Zakeri Z, Quaglino D, Latham T, Lockshin R (1993) Delayed internucleosomal DNA framgentation in programmed cell death. FASEB J 7: 470–478

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Department of Neurosurgery, Osaka University Medical School, Osaka, Japan

    T. Sakaki, E. Kohmura, T. Kishiguchi, T. Yuguchi, T. Yamashita & T. Hayakawa

Authors

  1. T. Sakaki

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  2. E. Kohmura

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  3. T. Kishiguchi

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  4. T. Yuguchi

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  5. T. Yamashita

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  6. T. Hayakawa

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Sakaki, T., Kohmura, E., Kishiguchi, T. et al. Loss and apoptosis of smooth muscle cells in intracranial aneurysms studies with in situ DNA end labeling and antibody against single-stranded DNA. Acta neurochir 139, 469–475 (1997). https://doi.org/10.1007/BF01808885

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  • Issue Date: May 1997

  • DOI: https://doi.org/10.1007/BF01808885

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