link.springer.com

Identification ofTnr3, aSuppressor-Mutator/Enhancer-like transposable element from rice - Molecular Genetics and Genomics

  • ️Ohtsubo, Hisako
  • ️Thu Feb 01 1996

Abstract

We isolated members of the retroposon family p-SINE1 in rice and found that one member contained an insertion. A 3-bp sequence at the insertion site within p-SINE1 appeared duplicated. The insertion sequence, 1536 bp in length, carried imperfect inverted repeats of about 13 bp at its termini which begin with 5′-CACTA--- -3′; these repeats are similar to those found in members of theEn/Spm transposable element family. These results indicate that the insertion sequence is a transposable element belonging to theEn/Spm family and is thus namedTnr3 (transposable element inrice no.3). In fact,Tnr3 carried long subterminal regions containing direct and inverted repeats of short DNA sequences of 15 bp, another characteristic of theEN/Spm family. The subterminal repeat sequences inTnr3 are, however, of two kinds, although they share homology with each other.Tnr3 and its relatives were present in multiple copies in rice. Considering the length ofTnr3, it cannot represent an autonomous type element, but is a non-autonomous element probably derived by deletion from an autonomous transposon.

Access this article

Log in via an institution

Subscribe and save

  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Fedoroff NV (1989) Maize transposable elements. In: Berg DE, Howe MM (eds) Mobile DNA. American Society for Microbiology. Washington DC, pp 375–411

    Google Scholar 

  • Frey M, Reinecke J, Grant S, Saedler H, Gierl A (1990) Excision of theEn/Spm transposable element ofZea mays requires two element-encoded proteins. EMBO J 9:4037–4044

    PubMed  Google Scholar 

  • Gierl A, Saedler H (1992) Plant-transposable elements and gene tagging. Plant Mol Biol 19:39–49

    Article  PubMed  Google Scholar 

  • Gierl A, Lütticke S, Saedler H (1988)TnpA product encoded by the transposable elementEn-1 ofZea mays is a DNA binding protein. EMBO J 7:4045–4053

    PubMed  Google Scholar 

  • Hoshino A, Inagaki Y, Iida S (1995) Structural analysis ofTpn1, a transposable element isolated from Japanese morning glory bearing variegated flowers. Mol Gen Genet 247:114–117

    Article  PubMed  Google Scholar 

  • Inagaki Y, Hisatomi Y, Suzuki T, Kasahara K, Iida S (1994) Isolation of aSuppressor-mutator/Enhancer-like transposable element,Tpn1, from Japanese morning glory bearing variegated flowers. Plant Cell 6:375–383

    Article  PubMed  Google Scholar 

  • Krebbers E, Hehl R, Piotrowiak R, Lönnig WE, Sommer H, Saedler H (1987) Molecular analysis of paramutant plants ofAntirrhinum majus and the involvement of transposable elements. Mol Gen Genet 209:499–507

    Article  Google Scholar 

  • Luo D, Coen ES, Doyle S, Carpenter R (1991) Pigmentation mutants produced by transposon mutagenesis inAntirrhinum majus. Plant J 1:59–69

    PubMed  Google Scholar 

  • Masson P, Surosky R, Kingsbury JA, Fedoroff NV (1987) Genetic and molecular analysis of theSpm-dependenta-m2 alleles of the maizea locus. Genetics 117:117–137

    PubMed  Google Scholar 

  • Masson P, Rutherford G, Banks JA, Fedoroff NV (1989) Essential large transcripts of the maizeSpm transposable element are generated by alternative splicing. Cell 58:755–765

    Article  PubMed  Google Scholar 

  • Masson P, Strem M, Fedoroff NV (1991) ThetnpA andtnpD gene products of theSpm element are required for transposition in tobacco. Plant Cell 3:73–85

    Article  PubMed  Google Scholar 

  • Messing J (1983) New M13 vectors in cloning. Methods Enzymol 101:20–78

    PubMed  Google Scholar 

  • Mochizuki K, Umeda M, Ohtsubo H, Ohtsubo E (1992) Characterization of a plant SINE, p-SINE1, in rice genomes. Jpn J Genet 57:155–166

    Article  Google Scholar 

  • Nacken WKF, Piotrowiak R, Saedler, Sommer H (1991) The transposable elementTam1 fromAntirrhinum majus shows structural homology to the maize transposonEn/Spm and has no sequence specificity of insertion. Mol Gen Genet 228:201–208

    Article  PubMed  Google Scholar 

  • Nevers P, Shepherd NS, Saedler H (1986) Plant transposable elements. Adv Bot Res 12:103–203

    Google Scholar 

  • Ohtsubo H, Umeda M, Ohtsubo E (1991) Organization of DNA sequences highly repeated in tandem in rice genomes. Jpn J Genet 66:241–254

    PubMed  Google Scholar 

  • Pereira A, Cuypers H, Schwarz-Sommer Z, Saedler H (1986) Molecular analysis of theEn/Spm transposable element system ofZea mays. EMBO J 5:835–841

    PubMed  Google Scholar 

  • Peterson P (1987) Mobile elements in plants. CRC Crit Rev Plant Sci 6:104–208

    Google Scholar 

  • Rhodes PR, Vodkin LO (1985) Highly structured sequence homology between an insertion element and the gene in which it resides. Proc Natl Acad Sci USA 82:493–497

    Google Scholar 

  • Rhodes PR, Vodkin LO (1988) Organization of theTgm family of transposable elements in soybean. Genetics 120:597–604

    PubMed  Google Scholar 

  • Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, Mullis KB, Erlich HA (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239:487–491

    PubMed  Google Scholar 

  • Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467

    PubMed  Google Scholar 

  • Shirsat AH (1988) A transposon-like structure in the 5′ flanking sequence of a legumin gene fromPisum sativum. Mol Gen Genet 212:129–133

    Article  PubMed  Google Scholar 

  • Trentmann SM, Saedler H, Gierl A (1993) The transposable elementEn/Spm-encoded TNPA protein contains a DNA binding and a dimerization domain. Mol Gen Genet 238:201–208

    PubMed  Google Scholar 

  • Umeda M, Ohtsubo H, Ohtsubo E (1991) Diversification of the riceWaxy gene by insertion of mobile DNA elements into introns. Jpn J Genet 66:569–586

    PubMed  Google Scholar 

  • Vieira J, Messing J (1987) Production of single stranded plasmid DNA. Methods Enzymol 153:3–11

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Institute of Molecular and Cellular Biosciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, 113, Tokyo, Japan

    Reiko Motohashi, Eiichi Ohtsubo & Hisako Ohtsubo

Authors

  1. Reiko Motohashi

    You can also search for this author in PubMed Google Scholar

  2. Eiichi Ohtsubo

    You can also search for this author in PubMed Google Scholar

  3. Hisako Ohtsubo

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by H. Saedler

About this article

Cite this article

Motohashi, R., Ohtsubo, E. & Ohtsubo, H. Identification ofTnr3, aSuppressor-Mutator/Enhancer-like transposable element from rice. Molec. Gen. Genet. 250, 148–152 (1996). https://doi.org/10.1007/BF02174173

Download citation

  • Received: 30 August 1995

  • Accepted: 02 October 1995

  • Issue Date: February 1996

  • DOI: https://doi.org/10.1007/BF02174173

Key words