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Protein factors in pre-mRNA 3′-end processing - Cellular and Molecular Life Sciences

  • ️Tong, L.
  • ️Wed Dec 26 2007

Abstract.

Most eukaryotic mRNA precursors (premRNAs) must undergo extensive processing, including cleavage and polyadenylation at the 3′-end. Processing at the 3′-end is controlled by sequence elements in the pre-mRNA (cis elements) as well as protein factors. Despite the seeming biochemical simplicity of the processing reactions, more than 14 proteins have been identified for the mammalian complex, and more than 20 proteins have been identified for the yeast complex. The 3′-end processing machinery also has important roles in transcription and splicing. The mammalian machinery contains several sub-complexes, including cleavage and polyadenylation specificity factor, cleavage stimulation factor, cleavage factor I, and cleavage factor II. Additional protein factors include poly(A) polymerase, poly(A)-binding protein, symplekin, and the C-terminal domain of RNA polymerase II largest subunit. The yeast machinery includes cleavage factor IA, cleavage factor IB, and cleavage and polyadenylation factor.

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Authors and Affiliations

  1. Department of Biological Sciences, Columbia University, New York, NY, 10027, USA

    C. R. Mandel, Y. Bai & L. Tong

Authors

  1. C. R. Mandel

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  2. Y. Bai

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  3. L. Tong

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Corresponding author

Correspondence to L. Tong.

Additional information

Received 11 October 2007; received after revision 17 November 2007; accepted 21 November 2007

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Cite this article

Mandel, C.R., Bai, Y. & Tong, L. Protein factors in pre-mRNA 3′-end processing. Cell. Mol. Life Sci. 65, 1099–1122 (2008). https://doi.org/10.1007/s00018-007-7474-3

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  • Published: 26 December 2007

  • Issue Date: April 2008

  • DOI: https://doi.org/10.1007/s00018-007-7474-3

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