link.springer.com

Immunocytochemical evidence for inducible nitric oxide synthase and cyclooxygenase-2 expression with nitrotyrosine formation in human hibernating myocardium - Basic Research in Cardiology

  • ️Camici, Paolo G.
  • ️Sun Sep 01 2002

Abstract.

Background: Myocardial hibernation may result from repetitive episodes of transient ischaemia leading to prolonged dysfunction. Inducible nitric oxide synthase (iNOS) expression has been demonstrated in animals following brief, non-lethal ischaemia-reperfusion injury. We therefore, hypothesised that in human hibernating myocardium: 1) iNOS would be present; 2) the reaction of nitric oxide and superoxide would form the strong oxidant peroxynitrite; 3) that this process would be accompanied by the expression of cyclooxygenase-2 (Cox-2) which interacts with NOS and whose products could further affect myocardial function. Method and results: In sixteen patients with coronary artery disease (CAD), left ventricular biopsies were obtained from chronically dysfunctional segments subtended by a stenotic artery (> 75 %) and shown to be viable by 18F-fluorodeoxyglucose positron emission tomography. Comparison was made with myocardial biopsies (n = 8) from normally contracting myocardium in patients undergoing coronary surgery, from unused transplant donors and at post-mortem. Regional wall motion score improved in all patients 6 months post-revascularisation (from 2.7 ± 0.7 to 1.5 ± 0.5; p < 0.001), confirming hibernation. Immunocytochemistry localized reactivity to iNOS, Cox-2 and nitrotyrosine (a marker of peroxynitrite formation) to cardiomyocytes from hibernating segments. No difference in reactivity to endothelial NOS was seen between hibernating and control cardiomyocytes. Conclusion: Cox-2 and iNOS are co-expressed in hibernating myocardium with nitrotyrosine suggesting nitric oxide production and peroxynitrite formation. We propose that this is secondary to ischaemia-reperfusion and that the products of these enzymes may have consequences for myocardial contractile function and survival.

Access this article

Log in via an institution

Subscribe and save

  • Get 10 units per month
  • Download Article/Chapter or eBook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

Author information

Authors and Affiliations

  1. MRC Clinical Sciences Centre, Imperial College Faculty of Medicine, Hammersmith Hospital, Du Cane Road, London, W12 ONN United Kingdom, Tel.: +(44) 208 383 3186, Fax: +(44) 208 383 3742, E-Mail: paolo.camici@csc.mrc.ac.uk, , , , , , GB

    Paolo G. Camici

  2. Department of Cardiology, Faculty of Medicine, Imperial College of Science, Technology and Medicine, Hammersmith Hospital, London, , , , , , GB

    Christopher S. R. Baker & Roger J. C. Hall

  3. Department of Histochemistry, Faculty of Medicine, Imperial College of Science, Technology and Medicine, Chelsea and Westminster Hospital, London, , , , , , GB

    Julia M. Polak

  4. MRC Clinical Sciences Centre, Faculty of Medicine, Imperial College of Science, Technology and Medicine, Hammersmith Hospital, London, , , , , , GB

    David P. Dutka & Ornella Rimoldi

  5. Department of Cardiothoracic Surgery, Queen Elizabeth Hospital, Birmingham, United Kingdom, , , , , , GB

    Domenico Pagano, Michael Pitt & Robert S. Bonser

Authors

  1. Christopher S. R. Baker

    You can also search for this author in PubMed Google Scholar

  2. David P. Dutka

    You can also search for this author in PubMed Google Scholar

  3. Domenico Pagano

    You can also search for this author in PubMed Google Scholar

  4. Ornella Rimoldi

    You can also search for this author in PubMed Google Scholar

  5. Michael Pitt

    You can also search for this author in PubMed Google Scholar

  6. Roger J. C. Hall

    You can also search for this author in PubMed Google Scholar

  7. Julia M. Polak

    You can also search for this author in PubMed Google Scholar

  8. Robert S. Bonser

    You can also search for this author in PubMed Google Scholar

  9. Paolo G. Camici

    You can also search for this author in PubMed Google Scholar

Additional information

Received: 11 February 2002, Returned for revision: 14 February 2002, Revision received: 4 March 2002, Accepted: 11 March 2002

About this article

Cite this article

Baker, C., Dutka, D., Pagano, D. et al. Immunocytochemical evidence for inducible nitric oxide synthase and cyclooxygenase-2 expression with nitrotyrosine formation in human hibernating myocardium. Basic Res Cardiol 97, 409–415 (2002). https://doi.org/10.1007/s003950200050

Download citation

  • Issue Date: September 2002

  • DOI: https://doi.org/10.1007/s003950200050