Genetic distinctiveness of brown pelicans (Pelecanus occidentalis) from the Galápagos Islands compared to continental North America - Conservation Genetics
- ️Friesen, Vicki
- ️Sat Dec 23 2017
Abstract
We examined population differentiation across a substantial portion of the range of the brown pelican (Pelecanus occidentalis) to assess (1) the genetic distinctness of the Galápagos subspecies (P. o. urinator) and (2) genetic differentiation between subspecies that inhabit the coasts of North and Central America (P. o. californicus and P. o. carolinensis). Birds were sampled from coastal California, coastal Florida, and the Galápagos Islands. Using a 957 bp (bp) fragment of the NADH dehydrogenase subunit 2 (ND2) gene, 661 bp of the mitochondrial control region, and eleven microsatellite loci we characterize population genetic differentiation among 158 brown pelicans. The Galápagos subspecies is genetically distinct from the sampled continental subspecies, possessing a unique ND2 haplotype and unique mitochondrial control region haplotypes. Samples from the two continental subspecies all possessed the same ND2 haplotype and shared four mitochondrial control region haplotypes. Bayesian clustering in STRUCTURE placed the Galápagos subspecies in a distinct genetic group with high probability, but could not differentiate the continental subspecies from one another. Estimates of migration rates from BayesAss indicated substantial migration between continental subspecies, but no migration between the Galápagos subspecies and either continental subspecies. There are clearly two Evolutionarily Significant Units within the range of the brown pelican, which warrants conservation attention. Further investigation should determine how the un-sampled subspecies (P. o. murphyi and P. o. occidentalis) fit into the broader picture.
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Acknowledgements
Thanks to Dan Anderson, B. Ploger and P. Boag for sample collection; to the Galápagos National Park Directorate (GNPD) for permission to work in the Park; to the Charles Darwin Foundation (CDF) and TAME Airline for logistical support; and to the Zhengxin Sun for microsatellite analyses, Genome Quebec Innovation Center for sequencing, and Savanah River Ecology Laboratory (S. Lance) for microsatellite development. Thanks to W. Hochachka, J. Morris-Pocock, S. Wallace, E. Chown, and C. Eckert for lab and data analysis help and project discussion. Thanks to the rangers from the GNPD and the assistants from CDF who helped in the field in Galápagos. Thanks to the USNM for the Panama specimen tissue loan. This work was funded by an NSERC Discovery grant (VLF), an NSERC PGSD (SAT), and Queen’s University Summer Work Experience Program. This material is based upon work supported by the National Science Foundation under Grant No. DEB 0842199 to DJA. This publication is a contribution of the Charles Darwin Foundation for the Galápagos Islands.
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Scott A. Taylor
Present address: Department of Ecology and Evolutionary Biology, University of Colorado Boulder, 1300 Pleasant Street, Boulder, CO, 80302, USA
Authors and Affiliations
Department of Biology, Queen’s University, Kingston, ON, Canada
Scott A. Taylor, Melissa Jenkins, Meera Manghani, Tim Birt & Vicki Friesen
Department of Biology, Wake Forest University, Winston-Salem, NC, USA
David J. Anderson
Charles Darwin Research Station, Puerto Ayora, Isla Santa Cruz, Galápagos, Ecuador
Gustavo Jiménez-Uzcátegui
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- Melissa Jenkins
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- Meera Manghani
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- Tim Birt
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- David J. Anderson
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- Gustavo Jiménez-Uzcátegui
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- Vicki Friesen
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SAT and VLF conceived the ideas; SAT, MJ, MM, TB, GJU and DJA collected the data; SAT, MJ, and MM analyzed the data; VLF and DJA funded the project; and SAT led the writing with the help of all authors.
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Correspondence to Scott A. Taylor.
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This research was conducted in compliance with the Guidelines to the use of Wild Birds in Research and adhering to V. L. Friesen’s Animal Care Protocol.
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Taylor, S.A., Jenkins, M., Manghani, M. et al. Genetic distinctiveness of brown pelicans (Pelecanus occidentalis) from the Galápagos Islands compared to continental North America. Conserv Genet 19, 629–636 (2018). https://doi.org/10.1007/s10592-017-1041-8
Received: 16 June 2017
Accepted: 18 December 2017
Published: 23 December 2017
Issue Date: June 2018
DOI: https://doi.org/10.1007/s10592-017-1041-8