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A study on the role that quorum sensing play in antibiotic-resistant plasmid conjugative transfer in Escherichia coli - Ecotoxicology

  • ️Yu, Yang
  • ️Fri Jan 19 2018

Abstract

Horizontal genes transfer (HGT) plays an important role in the dissemination of antibiotic resistance genes (ARGs) in the environment. However, the mechanisms of HGT of ARGs under the influence of antibiotics in sub-MIC remain rarely explored. Moreover, given its collective nature, HGT was considered to be relative to quorum sensing (QS) system. To investigate whether QS has any impact on horizontal gene transfer of ARGs, experiments were conducted to determine the conjugative efficiency of plasmid RP4 on Escherichia coli (E.coli) under the influences of tetracyclines (TCs), quorum sensing autoinducers (AIs) and quorum sensing inhibitors (QSIs). The results indicated that the sub-MIC TCs could facilitate the conjugative transfer of RP4, a process which could be enhanced by AIs but inhibited by QSIs. This study demonstrated the roles that QS played in the dissemination of ARGs, and provided theoretical insights into the mechanism of HGT of ARGs in the environment.

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Funding

This work was funded by the Foundation of the State Key Laboratory of Pollution Control and Resource Reuse, China (PCRRK16007), the National Natural Science Foundation of China (21577105, 21777123), the National Water Pollution Control and Treatment Science and Technology Major Project of China (2018ZX07109-1), the Science & Technology Commission of Shanghai Municipality (14DZ2261100, 17DZ1200103), the State Key Laboratory of Environmental Chemistry and Ecotoxicology (KF2016-11), and the 111 Project.

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Author notes

  1. Yueheng Zhang and Qingping Ma contributed equally to this work and are co-first authors.

Authors and Affiliations

  1. State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, 200092, Shanghai, P.R. China

    Yueheng Zhang, Qingping Ma, Rui Chen, Zhifen Lin & Dali Wang

  2. College of Marine Ecology and Environment, Shanghai Ocean University, 201306, Shanghai, P.R. China

    Yueheng Zhang & Qingping Ma

  3. Fujian Provincial Key Laboratory of Marine Enzyme Engineering, Fuzhou University, Fuzhou, 350116, P.R. China

    Bingmei Su & Juan Lin

  4. Shanghai Institute of Pollution Control and Ecological Security, 200092, Shanghai, P.R. China

    Zhifen Lin

  5. Shanghai Key Lab of Chemical Assessment and Sustainability, Shanghai, 201306, P.R. China

    Zhifen Lin

  6. Solid Waste and Chemicals Management Center, Ministry of Environmental Protection, 100029, Beijing, P.R. China

    Yang Yu

Authors

  1. Yueheng Zhang

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  2. Qingping Ma

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  3. Bingmei Su

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  4. Rui Chen

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  5. Juan Lin

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  6. Zhifen Lin

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  7. Dali Wang

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  8. Yang Yu

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Corresponding author

Correspondence to Zhifen Lin.

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The authors declare that they have no conflict of interest.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Zhang, Y., Ma, Q., Su, B. et al. A study on the role that quorum sensing play in antibiotic-resistant plasmid conjugative transfer in Escherichia coli. Ecotoxicology 27, 209–216 (2018). https://doi.org/10.1007/s10646-017-1886-0

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  • Accepted: 08 December 2017

  • Published: 19 January 2018

  • Issue Date: March 2018

  • DOI: https://doi.org/10.1007/s10646-017-1886-0

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