A study on the role that quorum sensing play in antibiotic-resistant plasmid conjugative transfer in Escherichia coli - Ecotoxicology
- ️Yu, Yang
- ️Fri Jan 19 2018
Abstract
Horizontal genes transfer (HGT) plays an important role in the dissemination of antibiotic resistance genes (ARGs) in the environment. However, the mechanisms of HGT of ARGs under the influence of antibiotics in sub-MIC remain rarely explored. Moreover, given its collective nature, HGT was considered to be relative to quorum sensing (QS) system. To investigate whether QS has any impact on horizontal gene transfer of ARGs, experiments were conducted to determine the conjugative efficiency of plasmid RP4 on Escherichia coli (E.coli) under the influences of tetracyclines (TCs), quorum sensing autoinducers (AIs) and quorum sensing inhibitors (QSIs). The results indicated that the sub-MIC TCs could facilitate the conjugative transfer of RP4, a process which could be enhanced by AIs but inhibited by QSIs. This study demonstrated the roles that QS played in the dissemination of ARGs, and provided theoretical insights into the mechanism of HGT of ARGs in the environment.
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Calabrese EJ, Hoffmann GR, Stanek EJ et al. (2010) Hormesis in high-throughput screening of antibacterial compounds in E coli. Hum Exp Toxicol 29(8):667
Covo V, Medina LM, Vives MJ, et al. (2008) Quorum Quenching on Pseudomonas Aeruginosa: Optimization of the Dose of 4-Nitropyridine-N-Oxide for the Inhibition of Biofilms Based on Stochastic Kinetics. Aiche Meeting
Dunny GM (2013) Enterococcal sex pheromones: signaling, social behavior, and evolution. Annu Rev Genet 47(47):457–482
González Barrios AF, Zuo R, Hashimoto Y et al. (2006) Autoinducer 2 controls biofilm formation in Escherichia coli through a novel motility quorum-sensing regulator (MqsR, B3022). J Bacteriol 188(1):305
Huddleston JR (2014) Horizontal gene transfer in the human gastrointestinal tract: potential spread of antibiotic resistance genes. Infect Drug Resist 7:167–176
Hwang I, Zhang L, Piper KR et al. (1994) TraI, a LuxI Homologue, is Responsible for Production of Conjugation Factor, the Ti Plasmid N-Acylhomoserine Lactone Autoinducer. Proc Natl Acad Sci USA 91(11):4639–4643
Ji X, Shen Q, Liu F et al. (2012) Antibiotic resistance gene abundances associated with antibiotics and heavy metals in animal manures and agricultural soils adjacent to feedlots in Shanghai; China. J Hazard Mater 235-236(20):178
Keeney D, Ruzin A, Mcaleese F et al. (2008) MarA-mediated overexpression of the AcrAB efflux pump results in decreased susceptibility to tigecycline in Escherichia coli. J Antimicrob Chemother 61(1):46
Lei B, Zhang K, Shen D et al. (2017) Control of the pollution of antibiotic resistance genes in soils by quorum sensing inhibition. Environ Sci Pollut Res 24(6):5259–5267
Lessl M, Balzer D, Pansegrau W et al. (1992) Sequence similarities between the RP4 Tra2 and the Ti VirB region strongly support the conjugation model for T-DNA transfer. J Biol Chem 267(28):20471
Luo Y, Xu L, Rysz M et al. (2011) Occurrence and transport of tetracycline, sulfonamide, quinolone, and macrolide antibiotics in the Haihe River Basin, China. Environ Sci Technol 45(5):1827–1833
Muñoz-Aguayo J, Lang KS, Lapara TM et al. (2007) Evaluating the effects of chlortetracycline on the proliferation of antibiotic-resistant bacteria in a simulated river water ecosystemAppl Environ Microbiol 73(17):5421–5425
Norman A, Hansen LH, Sørensen SJ (2009) Conjugative plasmids: vessels of the communal gene pool. Philos Trans R Soc Lond 364(1527):2275
Pansegrau W, Balzer D, Kruft V et al. (1990) In vitro assembly of relaxosomes at the transfer origin of plasmid RP4. Proc Natl Acad Sci USA 87(17):6555–6559
Ploy MC, Lambert T, Couty JP et al. (2000) Integrons: an antibiotic resistance gene capture and expression system. Clin Chem Lab Med 38(6):483–487
Ponnusamy K, Paul D, Sam KY et al. (2010) 2(5H)-Furanone: a prospective strategy for biofouling-control in membrane biofilm bacteria by quorum sensing inhibition. Braz J Microbiol 41(1):227–234
Qiu Z, Yu Y, Chen Z et al. (2012) Nanoalumina promotes the horizontal transfer of multiresistance genes mediated by plasmids across genera. Proc Natl Acad Sci USA 109(13):4944–4949
Shetye GS, Singh N, Gao X et al. (2013) Structures and biofilm inhibition activities of brominated furanones for Escherichia coli and Pseudomonas aeruginosa. Medchemcomm 4(7):1079–1084
Su S, Khan SR, Farrand SK (2008) Induction and loss of Ti plasmid conjugative competence in response to the acyl-homoserine lactone quorum-sensing signal. J Bacteriol 190(13):4398–4407
Thiele-Bruhn S (2003) Pharmaceutical antibiotic compounds in soils-a review. J Plant Nutr Soil Sci 166(2):145–167
Thienfah C, O’Toole GA (2001) Mechanisms of biofilm resistance to antimicrobial agents. Trends Microbiol 9(1):34–39
Tsui WH, Yim G, Wang HH et al. (2004) Dual effects of MLS antibiotics: transcriptional modulation and interactions on the ribosome. Chem Biol 11(9):1307–1316
Wei Y, Lee JM, Smulski DR et al. (2001) Global impact of sdiA amplification revealed by comprehensive gene expression profiling of Escherichia coli. J Bacteriol 183(7):2265
Weir EK, Martin LC, Poppe C et al. (2008) Subinhibitory concentrations of tetracycline affect virulence gene expression in a multi-resistant Salmonella enterica subsp. enterica serovar Typhimurium DT104. Microbes & Infect 10(8):901–907
Wong JJ, Lu J, Glover JN (2012) Relaxosome function and conjugation regulation in F-like plasmids—a structural biology perspective. Mol Microbiol 85(4):602–617
Zhang Y, Gu AZ, He M et al. (2017) Sub-inhibitory concentrations of disinfectants promote the horizontal transfer of multidrug resistance genes within and across Genera. Environ Sci Technol 51(1):570
Zhou LJ, Ying GG, Zhao JL et al (2011) Trends in the occurrence of human and veterinary antibiotics in the sediments of the Yellow River, Hai River and Liao River in northern China Environ Pollut 159(7):1877–1885
Funding
This work was funded by the Foundation of the State Key Laboratory of Pollution Control and Resource Reuse, China (PCRRK16007), the National Natural Science Foundation of China (21577105, 21777123), the National Water Pollution Control and Treatment Science and Technology Major Project of China (2018ZX07109-1), the Science & Technology Commission of Shanghai Municipality (14DZ2261100, 17DZ1200103), the State Key Laboratory of Environmental Chemistry and Ecotoxicology (KF2016-11), and the 111 Project.
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Yueheng Zhang and Qingping Ma contributed equally to this work and are co-first authors.
Authors and Affiliations
State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, 200092, Shanghai, P.R. China
Yueheng Zhang, Qingping Ma, Rui Chen, Zhifen Lin & Dali Wang
College of Marine Ecology and Environment, Shanghai Ocean University, 201306, Shanghai, P.R. China
Yueheng Zhang & Qingping Ma
Fujian Provincial Key Laboratory of Marine Enzyme Engineering, Fuzhou University, Fuzhou, 350116, P.R. China
Bingmei Su & Juan Lin
Shanghai Institute of Pollution Control and Ecological Security, 200092, Shanghai, P.R. China
Zhifen Lin
Shanghai Key Lab of Chemical Assessment and Sustainability, Shanghai, 201306, P.R. China
Zhifen Lin
Solid Waste and Chemicals Management Center, Ministry of Environmental Protection, 100029, Beijing, P.R. China
Yang Yu
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- Yueheng Zhang
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- Qingping Ma
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- Bingmei Su
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- Rui Chen
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- Juan Lin
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- Zhifen Lin
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- Dali Wang
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- Yang Yu
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Correspondence to Zhifen Lin.
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Zhang, Y., Ma, Q., Su, B. et al. A study on the role that quorum sensing play in antibiotic-resistant plasmid conjugative transfer in Escherichia coli. Ecotoxicology 27, 209–216 (2018). https://doi.org/10.1007/s10646-017-1886-0
Accepted: 08 December 2017
Published: 19 January 2018
Issue Date: March 2018
DOI: https://doi.org/10.1007/s10646-017-1886-0