The anti-HSV-1 effect of quercetin is dependent on the suppression of TLR-3 in Raw 264.7 cells - Archives of Pharmacal Research
- ️Cho, Hyosun
- ️Fri Mar 03 2017
Abstract
Quercetin is a major component of the plant Glycyrrhiza uralensis, which is largely used as a traditional medicine in Asia. Quercetin has been reported to have several biological activities, which include anti-viral and anti-inflammatory effects. We explored the molecular mechanism linking anti-viral and anti-inflammatory activities using an in vitro herpes simplex virus-1 (HSV-1) infection model. Raw 264.7 cells were infected with HSV-1 in the presence or absence of different concentrations of quercetin and infected cell lysates were harvested 24 h later. HSV plaque reduction assays, western blotting (HSV-1gD, HSV-1 ICP0, TLR-2, 3, 9, NF-κB, IRF3), and real time PCR (HSV-1ICP0, HSV-1UL13, HSV-1UL52) were performed to elucidate the mechanism responsible for the anti-HSV-1 effect of quercetin. In addition, TNF-α level was measured. Quercetin significantly lowered HSV infectivity in Raw 264.7 cells and inhibited the expressions of HSV proteins (gD, ICP0) and genes (ICP0, UL13, UL52). Interestingly, quercetin specifically suppressed the expression of TLR-3, and this led to the inhibitions of inflammatory transcriptional factors (NF-κB and IRF3). These findings suggest that the anti-HSV-1 effects of quercetin are related to the suppression of TLR-3 dependent inflammatory responses in Raw 264.7 cells.
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Acknowledgements
We gratefully acknowledge the financial support of the National Research Foundation of Korea (NRF) (2015R1C1A1A02037573). This work was also supported by the Priority Research Center Program through the NRF funded by the Korean Ministry of Education, Science and Technology (2016R1A6A1A03007648).
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Authors and Affiliations
College of Pharmacy, Duksung Women’s University, Seoul, 132-714, Republic of Korea
Seulki Lee, Hwan Hee Lee & Hyosun Cho
Innovative Drug Center, Duksung Women’s University, Seoul, 132-714, Republic of Korea
Seulki Lee, Hwan Hee Lee & Hyosun Cho
Department of Medicinal Crop Research, National Institute of Horticultural and Herbal Science, Rural Development Administration, Eumseong, 369-873, Republic of Korea
Yu Su Shin
College of Pharmacy, Research Institute of Pharmaceutical Sciences and Institute for Microorganisms, Kyungpook National University, Daegu, 702-701, Republic of Korea
Hyojeung Kang
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- Hwan Hee Lee
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- Yu Su Shin
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- Hyojeung Kang
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- Hyosun Cho
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Correspondence to Yu Su Shin, Hyojeung Kang or Hyosun Cho.
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Lee, S., Lee, H.H., Shin, Y.S. et al. The anti-HSV-1 effect of quercetin is dependent on the suppression of TLR-3 in Raw 264.7 cells. Arch. Pharm. Res. 40, 623–630 (2017). https://doi.org/10.1007/s12272-017-0898-x
Received: 09 May 2016
Accepted: 12 February 2017
Published: 03 March 2017
Issue Date: May 2017
DOI: https://doi.org/10.1007/s12272-017-0898-x