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Carbon nanofiber multiplexed array and wireless instantaneous neurotransmitter concentration sensor for simultaneous detection of dissolved oxygen and dopamine - Biomedical Engineering Letters

  • ️Lee, Kendall H.
  • ️Tue Jan 08 2013

Abstract

Purpose

While the mechanism of Deep Brain Stimulation (DBS) remains poorly understood, previous studies have shown that it evokes release of neurochemicals and induces activation of functional magnetic resonance imaging (fMRI) blood oxygen level-dependent signal in distinct areas of the brain. Therefore, the main purpose of this paper is to demonstrate the capabilities of the Wireless Instantaneous Neurotransmitter Concentration Sensor system (WINCS) in conjunction with a carbon nanofiber (CNF) multiplexed array electrode as a powerful tool for elucidating the mechanism of DBS through the simultaneous detection of multiple bioactivemolecules.

Methods

Patterned CNF nanoelectrode arrays were prepared on a 4-inch silicon wafer where each device consists of 3 × 3 electrode pads, 200 μm square, that contain CNFs spaced at 1 μm intervals. The multiplexed carbon nanofiber CNF electrodes were integrated with WINCS to detect mixtures of dopamine (DA) and oxygen (O2) using fast scan cyclic voltammetry (FSCV) in vitro.

Results

First, simultaneous detection of O2 at two spatially different locations, 200 um apart, was demonstrated. Second, simultaneous detection of both O2 and DA at two spatially different locations, using two different decoupled waveforms was demonstrated. Third, controlled studies demonstrated that the waveform must be interleaved to avoid electrode crosstalk artifacts in the acquired data.

Conclusions

Multiplexed CNF nanoelectrode arrays for electrochemical detection of neurotransmitters show promise for the detection of multiple analytes with the application of time independent decoupled waveforms. Electrochemistry on CNF electrodes may be helpful in elucidating the mechanism of DBS, and may also provide the precision and sensitivity required for future applications in feedback modulated DBS neural control systems.

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Author information

Authors and Affiliations

  1. Department of Neurosurgery, Mayo Clinic, Rochester, MN, 55905, USA

    Michael P. Marsh, Kevin E. Bennet & Kendall H. Lee

  2. Center for Nanotechnology, NASA Ames Research Center, Moffett Field, CA, 94035, USA

    Jessica E. Koehne, Russell J. Andrews & M. Meyyappan

  3. Division of Engineering, Mayo Clinic, Rochester, MN, 55905, USA

    Kevin E. Bennet

  4. Department of Physiology and Biomedical Engineering, Mayo Clinic, Rochester, MN, 55905, USA

    Kendall H. Lee

Authors

  1. Michael P. Marsh

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  2. Jessica E. Koehne

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  3. Russell J. Andrews

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  4. M. Meyyappan

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  5. Kevin E. Bennet

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  6. Kendall H. Lee

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Corresponding author

Correspondence to Kendall H. Lee.

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Cite this article

Marsh, M.P., Koehne, J.E., Andrews, R.J. et al. Carbon nanofiber multiplexed array and wireless instantaneous neurotransmitter concentration sensor for simultaneous detection of dissolved oxygen and dopamine. Biomed. Eng. Lett. 2, 271–277 (2012). https://doi.org/10.1007/s13534-012-0081-8

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  • Received: 27 September 2012

  • Revised: 21 December 2012

  • Accepted: 23 December 2012

  • Published: 08 January 2013

  • Issue Date: December 2012

  • DOI: https://doi.org/10.1007/s13534-012-0081-8

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