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Genetics of Growth in Axenic Medium of the Cellular Slime Mould Dictyostelium discoideum - Nature

  • ️NEWELL, PETER C.
  • ️Fri Jan 18 1974
  • Letter
  • Published: 18 January 1974

Nature volume 247, pages 142–143 (1974)Cite this article

Abstract

ALTHOUGH Dictyostelium discoideum is normally grown on bacteria1,2, it is desirable for most biochemical studies to use strains grown in the absence of bacteria (axenically)3. Isolation of these strains is laborious, however; for this reason we have investigated the genetic basis of growth in axenic medium and have developed a method for inserting the chromosomes bearing the genes for axenic growth into other non-axenic strains. This is made possible by our finding that genes for axenic growth are located on only two of the seven linkage groups and that chromosomes can be readily exchanged between different strains. Using an improved version of the methods employing parasexuality previously reported4,5, we have selected axenic resegregants carrying various markers from non-axenic strains.

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Author information

Author notes

  1. RICHARD H. KESSIN

    Present address: Division of Biological Sciences, Brown University, Providence, Rhode Island, USA

Authors and Affiliations

  1. Department of Biochemistry, University of Oxford, South Parks Road, Oxford

    KEITH LESLIE WILLIAMS, RICHARD H. KESSIN & PETER C. NEWELL

Authors

  1. KEITH LESLIE WILLIAMS

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  2. RICHARD H. KESSIN

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  3. PETER C. NEWELL

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WILLIAMS, K., KESSIN, R. & NEWELL, P. Genetics of Growth in Axenic Medium of the Cellular Slime Mould Dictyostelium discoideum. Nature 247, 142–143 (1974). https://doi.org/10.1038/247142a0

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  • Received: 06 September 1973

  • Issue Date: 18 January 1974

  • DOI: https://doi.org/10.1038/247142a0