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A yeast gene necessary for bud-site selection encodes a protein similar to insulin-degrading enzymes - Nature

  • ️Misumi, Yoshio
  • ️Thu Dec 08 1994
  • Letter
  • Published: 08 December 1994

Nature volume 372, pages 567–570 (1994)Cite this article

Abstract

CELLS of the yeast Saccharomyces cerevisiae choose bud sites in a non-random spatial pattern that depends on mating type: axial for haploid cells and bipolar for a/α diploid cells1,2. We identified a mutant yeast, axi1, in which the budding pattern is altered from axial to bipolar. Expression of the AXLI gene is repressed in a/a diploid cells. With the ectopic expression of AXL1, a/α cells exhibited an axial budding pattern, thus AXL1 is a key morphological determinant that distinguishes the budding pattern of haploid cells from that of a/α diploid cells2. AXL1 encodes a protein similar in sequence to the human and Drosophila insulin-degrading enzymes3,4 and to the Escherichia coli ptr gene product5. The axial budding pattern might result from degradation of a target protein by the putative Axil protease.

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Author information

Author notes

  1. Chitoshi Oka: Industrial Research Institute of Chiba Prefecture, Kasori, Wakaba-ku, Chiba 264, Japan

  2. Yukihiko Arikawa: Nagano State Laboratory of Food Technology, Nishibannba, Kurita, Nagano 380, Japan

  3. Akio Tonouchi and Satoru Kuhara: Graduate School of Genetic Resources Technology, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812, Japan

  4. Yoshio Misumi: Department of Biochemistry, School of Medicine, Fukuoka University, 7-45-1 Nanakuma, Jonan-ku, Fukuoka 814-01, Japan

Authors and Affiliations

  1. National Institute of Bioscience and Human-Technology, Agency of Industrial Science & Technology, 1-1 Higashi, Tsukuba, 305, Japan

    Atsushi Fujita, Chitoshi Oka, Yukihiko Arikawa, Tatsuyuki Katagai, Akio Tonouchi, Satoru Kuhara & Yoshio Misumi

Authors

  1. Atsushi Fujita

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  2. Chitoshi Oka

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  3. Yukihiko Arikawa

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  4. Tatsuyuki Katagai

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  5. Akio Tonouchi

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  6. Satoru Kuhara

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  7. Yoshio Misumi

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Fujita, A., Oka, C., Arikawa, Y. et al. A yeast gene necessary for bud-site selection encodes a protein similar to insulin-degrading enzymes. Nature 372, 567–570 (1994). https://doi.org/10.1038/372567a0

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  • Received: 16 May 1994

  • Accepted: 31 October 1994

  • Issue Date: 08 December 1994

  • DOI: https://doi.org/10.1038/372567a0