Commensal microbe-derived butyrate induces the differentiation of colonic regulatory T cells - Nature

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DDBJ/GenBank/EMBL

Gene Expression Omnibus

Data deposits

The microarray and ChIP-seq analysis data have been deposited at the Gene Expression Omnibus (GEO) under accession number GSE49655. The microbiome analysis data have been deposited at the DDBJ database (http://getentry.ddbj.nig.ac.jp/) under accession number DRA001105.

• 12 February 2014

  An Erratum to this paper has been published: https://doi.org/10.1038/nature13041

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Author notes

1. Yukihiro Furusawa, Yuuki Obata, Shinji Fukuda and Koji Hase: These authors contributed equally to this work.

Authors and Affiliations

1. RIKEN Center for Integrative Medical Sciences (IMS-RCAI), Kanagawa 230-0045, Japan ,

   Yukihiro Furusawa, Yuuki Obata, Shinji Fukuda, Takaho A. Endo, Gaku Nakato, Daisuke Takahashi, Chikako Uetake, Keiko Kato, Tamotsu Kato, Masumi Takahashi, Eiji Miyauchi, Koji Atarashi, Satoshi Onawa, Shohei Hori, Osamu Ohara, Haruhiko Koseki, Kenya Honda, Koji Hase & Hiroshi Ohno

2. The Institute of Medical Science, The University of Tokyo, Tokyo 108-8639, Japan ,

   Yukihiro Furusawa, Yuuki Obata, Yumiko Fujimura & Koji Hase

3. Graduate School of Medicine, Chiba University, Chiba 260-8670, Japan ,

   Yuuki Obata, Haruhiko Koseki & Hiroshi Ohno

4. Institute for Advanced Biosciences, Keio University, Yamagata 997-0052, Japan ,

   Shinji Fukuda, Yumiko Nakanishi, Noriko N. Fukuda, Shinnosuke Murakami & Masaru Tomita

5. Graduate School of Medical Life Science, Yokohama City University, Kanagawa 230-0045, Japan ,

   Keiko Kato, Haruhiko Koseki, Jun Kikuchi & Hiroshi Ohno

6. Faculty of Agriculture, Shizuoka University, Shizuoka 422-8529, Japan ,

   Shingo Hino & Tatsuya Morita

7. PRESTO, Japan Science and Technology Agency, Saitama 332-0012, Japan ,

   Koji Atarashi & Koji Hase

8. Preventative Health National Research Flagship, CSIRO Food and Nutritional Sciences, South Australia 5000, Australia ,

   Trevor Lockett, Julie M. Clarke & David L. Topping

9. RIKEN Center for Sustainable Resource Science, Kanagawa 230-0045, Japan ,

   Jun Kikuchi

10. CREST, Japan Science and Technology Agency, Saitama 332-0012, Japan ,

    Kenya Honda

Contributions

S.F., K.Ha., D.L.T., T.M., K.Ho. and H.O. conceived the study; K.Ha. and S.F. designed the experiments and wrote the manuscript with Y.Fur., Y.O. and H.O.; Y. Fur. and Y.O conducted a large part of experiments together with S.F., G.N., D.T., C.U., K.K., T.K., M.Ta., E.M. and K.Ha; S.F, S.O. and K.Ha. prepared germ-free, CRB-associated and gnotobiotic mice. K.A. and K.Ho. were involved in data discussion. S.F., Y.N., C.U. and J.K. performed metabolome analysis. S.F., T.K., S.M. and M.To. performed microbiome analysis. T.A.E. performed bioinformatic analyses. S.Hi. and T.M. performed HPLC analysis. S.F. and N.N.F. performed GC–MS analysis. Y.Fuj. performed histological analysis. T.L., J.M.C., D.L.T. and S.Ho. provided essential materials and contributed to the design of experiments. Y.Fur. and H.K. contributed to the ChIP assay. H.O. directed the study and took primary responsibility for editing the manuscript.

Corresponding authors

Correspondence to Shinji Fukuda, Koji Hase or Hiroshi Ohno.

Competing interests

The authors declare no competing financial interests.

Cite this article

Furusawa, Y., Obata, Y., Fukuda, S. et al. Commensal microbe-derived butyrate induces the differentiation of colonic regulatory T cells. Nature 504, 446–450 (2013). https://doi.org/10.1038/nature12721

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• Received: 18 June 2013

• Accepted: 27 September 2013

• Published: 13 November 2013

• Issue Date: 19 December 2013

• DOI: https://doi.org/10.1038/nature12721