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Definition of a consensus binding site for p53 - Nature Genetics

  • ️Vogelstein, Bert
  • ️Wed Apr 01 1992
  • Article
  • Published: 01 April 1992

Nature Genetics volume 1, pages 45–49 (1992)Cite this article

Abstract

Recent experiments have suggested that p53 action may be mediated through its inter action with DNA. We have now identified 18 human genomic clones that bind to p53 in vitro. Precise mapping of the binding sequences within these clones revealed a consensus binding site with a striking internal symmetry, consisting of two copies of the 10 base pair motif 5′-PuPuPuC(A/T)(T/A)GPyPyPy-3′ separated by 0-13 base pairs. One copy of the motif was insufficient for binding, and subtle alterations of the motif, even when present in multiple copies, resulted in loss of affinity for p53. Mutants of p53, representing each of the four “hot spots” frequently altered in human cancers, failed to bind to the consensus dimer. These results define the DNA sequence elements with which p53 interacts in vitro and which may be important for p53 action in vivo.

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Authors and Affiliations

  1. Oncology Center, Johns Hopkins University School of Medicine, 424 N Bond Street, Baltimore, Maryland, 21231, USA

    Wafik S. El-Deiry, Scott E. Kern, Jennifer A. Pietenpol, Kenneth W. Kinzler & Bert Vogelstein

  2. Department of Pathology, Johns Hopkins University School of Medicine, 424 N Bond Street, Baltimore, Maryland, 21231, USA

    Scott E. Kern

Authors

  1. Wafik S. El-Deiry

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  2. Scott E. Kern

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  3. Jennifer A. Pietenpol

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  4. Kenneth W. Kinzler

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  5. Bert Vogelstein

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Cite this article

El-Deiry, W., Kern, S., Pietenpol, J. et al. Definition of a consensus binding site for p53. Nat Genet 1, 45–49 (1992). https://doi.org/10.1038/ng0492-45

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  • Received: 10 January 1992

  • Accepted: 24 February 1992

  • Issue Date: 01 April 1992

  • DOI: https://doi.org/10.1038/ng0492-45