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Hippocampal neurogenesis is not required for behavioral effects of environmental enrichment - Nature Neuroscience

  • ️Hen, René
  • ️Sun Apr 30 2006

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Acknowledgements

We thank J.A. Gordon, C.O. Lacefield and A. Sahay for their comments on the manuscript. This work was supported by US National Institute of Mental Health grants 5R01MH068542-03, 5T32MH018264-22 and 5F31MH069089-02.

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Author notes

  1. Dar Meshi and Michael R Drew: These authors contributed equally to this work.

Authors and Affiliations

  1. Department of Biological Sciences, Columbia University, New York, 10027, New York, USA

    Dar Meshi

  2. Center for Neurobiology and Behavior, Columbia University, 1051 Riverside Drive, Unit 87, New York, 10032, New York, USA

    Michael R Drew, Michael Saxe, Denis David, Luca Santarelli & René Hen

  3. Biopsychology Unit, New York State Psychiatric Institute, 1051 Riverside Drive, Unit 50, New York, 10032, New York, USA

    Michael R Drew & Chariklia Malapani

  4. Department of Psychiatry, Columbia University, 1051 Riverside Drive, Unit 14, New York, 10032, New York, USA

    Mark S Ansorge, Holly Moore & René Hen

  5. Faculté de Pharmacie, Université Paris-Sud, Tour D1, 2e etage, EA3544, Chatenay-Malabry, F-92296, Cedex, France

    Denis David

Authors

  1. Dar Meshi

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  2. Michael R Drew

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  3. Michael Saxe

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  4. Mark S Ansorge

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  5. Denis David

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  6. Luca Santarelli

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  7. Chariklia Malapani

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  8. Holly Moore

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  9. René Hen

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Corresponding author

Correspondence to René Hen.

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Supplementary information

Supplementary Fig. 1

CD68 immunoreactivity in the dentate gyrus and hilus of mice. (a) Irradiated mice exhibited significantly more CD68-positive cells than sham mice one month after x-irradiation (T(1,4) = 3.425, p = 0.027). No difference was observed between sham and irradiated groups two months after irradiation (T(1,4) = 0.079, p = 0.941). (b) Representative image showing CD68 positive cells in the dentate gyrus and hilus one month after irradiation. Error bars = 1 S.E.M. (PDF 2715 kb)

Supplementary Fig. 2

Images of (a) BrdU and (b) doublecortin immunohistochemistry in the dentate gyrus after housing in either standard or enriched environment, and treatment with either sham or hippocampal x-irradiation. Immunostaining of both BrdU and doublecortin is increased in enriched animals that received sham treatment (see Fig. 2). When animals in both standard and enriched housing received irradiation treatment, absence of BrdU and doublecortin immunostaining was observed. (PDF 28309 kb)

Supplementary Fig. 3

Proportion of time active in the habituation of activity test. On both days of the test enriched animals spent less time active (F's(1,60) > 14, p's < 0.001) and exhibited steeper within-session declines in activity (housing X minute interaction: F’s(2,120) > 13, p's < 0.001). There was no effect of irradiation treatment on either day (F’s(1,60) < 1), and irradiation did not interact significantly with the other variables. Error bars = 1 S.E.M. (PDF 467 kb)

Supplementary Fig. 4

Latency to feed in the novelty-suppressed feeding test. Male mice were transferred to the enriched environment immediately after irradiation and then tested after 6 weeks of enrichment. Enriched mice exhibit a significantly reduced latency to feed (F(1,75) = 36.647, p < 0.001). There was no effect of irradiation (F(1,75) = 0.112), and no interaction between housing and irradiation treatment (F(1,75) = 0.554). n = 20 per group. Error bars = 1 S.E.M. (PDF 465 kb)

Supplementary Methods (PDF 161 kb)

Supplementary Data (PDF 51 kb)

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Meshi, D., Drew, M., Saxe, M. et al. Hippocampal neurogenesis is not required for behavioral effects of environmental enrichment. Nat Neurosci 9, 729–731 (2006). https://doi.org/10.1038/nn1696

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  • Received: 01 February 2006

  • Accepted: 11 April 2006

  • Published: 30 April 2006

  • Issue Date: 01 June 2006

  • DOI: https://doi.org/10.1038/nn1696