Loss of Histone H3 Methylation at Lysine 4 Triggers Apoptosis in Saccharomyces cerevisiae
Figure 5
NUC1 disruption hampers apoptosis onset and improves viability of yeast cells lacking Set1p.
(A) Survival of WT, Δset1, Δnuc1 and Δset1Δnuc1 cells was determined by clonogenicity during chronological aging. Data represent mean ± SD (n = 3). (B) Integrals under the life span curves were determined. Data represent mean ± SD (n = 3, **P<0.01). (C) DHE-positive WT, Δset1, Δnuc1 and Δset1Δnuc1 cells were quantified after 4 days in culture by fluorescence microscopy. In each experiment, 800–1500 cells were evaluated. Data represent mean ± SD, ***P<0.001. (D) ROS accumulation in WT, Δyca1, Δdot1, Δset1, Δset1Δdot1 and Δset1Δdot1Δyca1 cells after two days in culture was determined by DHE staining and visualized by fluorescence microscopy. Scale bars, 10 µm.