Protein Arginine Methyltransferase 5 Functions in Opposite Ways in the Cytoplasm and Nucleus of Prostate Cancer Cells
Figure 2
PRMT5 contains three nuclear exclusion signals.
(A) Diagrams of the PRMT5 truncations expressed as GFP-fusion proteins. The percentages of cells with GFP-PRMT5 truncations in the cytoplasm (C), nucleus (N), or cytoplasm plus nucleus (C/N) are shown on the right. (B) Subcellular localization of isolated nuclear export signals. Cells were transfected with pcDNA-f:GFP-PRMT5, -PRMT5(1–90), -PRMT5(500–560), -PRMT5(576–637), or pcDNA-GFP and observed under a confocal microscope. (C) Western blot analysis of cytoplasmic and nuclear fractions of cells transfected with pcDNA-f:GFP-PRMT5, -PRMT5(1–90), PRMT5(500–560), or PRMT5(576–637) with anti-FLAG, -HSP90, or -lamin B antibody.