Protein Arginine Methyltransferase 5 Functions in Opposite Ways in the Cytoplasm and Nucleus of Prostate Cancer Cells
Figure 3
PRMT5 promotes p44 cytoplasmic translocation.
(A) Diagrams of the PRMT5 truncations. Cells were transfected with pcDNA-GFP-p44 and pcDNA-PRMT5 or pcDNA-PRMT5 truncations, and the percentages of cells with GFP-p44 in cytoplasm (C) or cytoplasm plus nucleus (C/N) are shown on the right. (B) Cytoplasmic translocation of GFP-p44 driven by PRMT5. Cells were transfected with pcDNA-GFP-p44 alone or together with pcDNA-f:PRMT5, -f:PRMT5(91–637), or f:PRMT5(325–637), and the GFP-p44 subcellular localization was observed under a confocal microscope. (C) Western blot analysis of cytoplasmic and nuclear fractions of Cos 7 cells described in B with anti-p44, -HSP90, or -lamin B antibody.