Protein Arginine Methyltransferase 5 Functions in Opposite Ways in the Cytoplasm and Nucleus of Prostate Cancer Cells
Figure 7
The nuclear PRMT5 inhibited the growth of prostate cancer cells.
(A) The PRMT5 protein in LNCaP cells expressing PRMT5, PRMT5mt, NLS-PRMT5, or NLS-PRMT5mt was immunostained with the anti-PRMT5 antibody. The samples were observed under a confocal microscope. (B) Western blot of cytoplasmic (C) and nuclear (N) fractions of LNCaP cells expressing PRMT5, f:PRMT5mt, NLS-PRMT5, or NLS-PRMT5mt with anti-PRMT5, -p44, -lamin B, or -HSP90 antibody. (C) Growth curves of prostate cancer LNCaP cells expressing PRMT5, f:PRMT5mt, NLS-PRMT5, or NLS-PRMT5mt.