The Housekeeping Gene Hypoxanthine Guanine Phosphoribosyltransferase (HPRT) Regulates Multiple Developmental and Metabolic Pathways of Murine Embryonic Stem Cell Neuronal Differentiation
Figure 1
HPRT knockdown in murine ESD3 cells.
(A) Reduction of HPRT mRNA expression as assayed by qPCR; (B) reduction of immunoreactive HPRT protein as determined by Western blot analysis; (C) reduction of HPRT enzymatic activity as measured by absent detectable IMP production as detected by autoradiography of a thin layer chromatography of cell lysate (6).