Mammalian In Vitro Splicing Assays
References
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Mayeda, A. and Ohshima, Y. (1990) β-globin transcripts carrying a single intron with three adjacent nucleotides of 5′ exon are efficiently spliced in vitro irrespective of intron position or surrounding exon sequences. Nucleic Acids Res. 18, 4671–4676.
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Krainer, A. R. and Maniatis, T. (1985) Multiple factors including the small nuclear ribonucleoproteins U1 and U2 are necessary for pre-mRNA splicing in vitro. Cell 42, 725–736.
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