Bcl-2 related proteins are dramatically induced at the early stage of differentiation in human liver cancer cells by a histone deacetylase inhibitor projecting an anti-apoptotic role during this period.
- Authors:
- K Wakabayashi
- H Saito
- H Ebinuma
- Y Saito
- T Takagi
- M Nakamura
- A Umezawa
- J Hata
- H Ishii
- Published online on: March 1, 2000 https://doi.org/10.3892/or.7.2.285
- Pages: 285-293
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Abstract
Expression of the Bcl-2 family members in a human hepatocellular carcinoma cell line (HCC-T) after sodium butyrate-treatment was investigated. Sodium butyrate, a histone deacetylase inhibitor, induced differentiation of the cell line into its normal counterpart without inducing apoptosis at the concentration of 2 mmol/l. Since sodium butyrate has effects on both differentiation and apoptosis, we investigated the expression profile of bcl-2 related genes in HCC-T. The expression of Bcl-2 and Mcl-1/EAT was up-regulated 4-12 h after the treatment while Bcl-XL was up-regulated 2-3 days after the stimulation. On the other hand, the expression levels of Bax protein remained unchanged during differentiation. The HCC-T cells entered a cell cycle arrest at G1 and showed neither cellular fragmentation nor apoptosis during this period, which was concomitantly associated with up-regulated expression of a cell cycle regulator, p21WAF-1. These results demonstrate that induction of anti-apoptotic bcl-2 related proteins at an early stage of differentiation is important for the maintenance of HCC-T cell differentiation by antagonizing pro-apoptotic molecules such as Bax.
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- Impact Factor: 3.8
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Oncology
(total number of cites) - CiteScore: 8.5
- CiteScore Rank: Q1: #74/404 Oncology
- Source Normalized Impact per Paper (SNIP): 0.719
- SCImago Journal Rank (SJR): 0.864
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Spandidos Publications style
Wakabayashi K, Saito H, Ebinuma H, Saito Y, Takagi T, Nakamura M, Umezawa A, Hata J and Ishii H: Bcl-2 related proteins are dramatically induced at the early stage of differentiation in human liver cancer cells by a histone deacetylase inhibitor projecting an anti-apoptotic role during this period.. Oncol Rep 7: 285-293, 2000.
APA
Wakabayashi, K., Saito, H., Ebinuma, H., Saito, Y., Takagi, T., Nakamura, M. ... Ishii, H. (2000). Bcl-2 related proteins are dramatically induced at the early stage of differentiation in human liver cancer cells by a histone deacetylase inhibitor projecting an anti-apoptotic role during this period.. Oncology Reports, 7, 285-293. https://doi.org/10.3892/or.7.2.285
MLA
Wakabayashi, K., Saito, H., Ebinuma, H., Saito, Y., Takagi, T., Nakamura, M., Umezawa, A., Hata, J., Ishii, H."Bcl-2 related proteins are dramatically induced at the early stage of differentiation in human liver cancer cells by a histone deacetylase inhibitor projecting an anti-apoptotic role during this period.". Oncology Reports 7.2 (2000): 285-293.
Chicago
Wakabayashi, K., Saito, H., Ebinuma, H., Saito, Y., Takagi, T., Nakamura, M., Umezawa, A., Hata, J., Ishii, H."Bcl-2 related proteins are dramatically induced at the early stage of differentiation in human liver cancer cells by a histone deacetylase inhibitor projecting an anti-apoptotic role during this period.". Oncology Reports 7, no. 2 (2000): 285-293. https://doi.org/10.3892/or.7.2.285