ProteomeXchange Dataset PXD023178
- ️Wed Mar 03 2021
PXD023178 is an original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Protein-protein interaction of Phytophthora infestans RxLR effector AvrCap1 by immunoafinity enrichment in planta |
| Description | In plants, NLR (nucleotide-binding domain and leucine-rich repeat-containing) proteins can form receptor networks to confer hypersensitive cell death and innate immunity. One class of NLRs, known as NRCs (NLR required for cell death), are central nodes in a complex network that protects against multiple pathogens and comprises up to half of the NLRome of solanaceous plants. Given the prevalence of this NLR network, we hypothesized that pathogens convergently evolved to secrete effectors that target NRC activities. To test this, we screened a library of 167 bacterial, oomycete, nematode and aphid effectors for their capacity to suppress the cell death response triggered by the NRC-dependent disease resistance proteins Prf and Rpi-blb2. Among five of the identified suppressors, one cyst nematode protein and one oomycete protein suppress the activity of autoimmune mutants of NRC2 and NRC3, but not NRC4, indicating that they specifically counteract a subset of NRC proteins independently of their sensor NLR partners. Whereas the cyst nematode effector SPRYSEC15 binds the nucleotide-binding domain of NRC2 and NRC3, the oomycete effector AVRcap1b suppresses the response of these NRCs via the membrane trafficking-associated protein NbTOL9a (Target of Myb 1-like protein 9a). The interaction was studied by co-immunoprecipitation in plantacoupled with tandem mass spectrometry (IP-MS). |
| HostingRepository | PRIDE |
| AnnounceDate | 2021-03-03 |
| AnnouncementXML | Submission_2021-03-02_22:30:23.299.xml |
| DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD023178 |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Supported dataset by repository |
| PrimarySubmitter | Jan Sklenar |
| SpeciesList | scientific name: Phytophthora infestans; NCBI TaxID: 4787; |
| ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
| Instrument | Orbitrap Fusion |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|---|---|---|
| 0 | 2020-12-16 02:49:49 | ID requested | |
| ⏵ 1 | 2021-03-02 22:30:23 | announced |
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Phytophthora infestans, RxLR effector, AvrCap1, RD54
Contact List
| Jan Sklenar | |
|---|---|
| contact affiliation | Proteomics group, The Sainsbury Laboratory, Colney lane, Norwich, NR4 7UH, United Kingdom |
| contact email | jan.sklenar@tsl.ac.uk |
| lab head | |
| Jan Sklenar | |
| contact affiliation | The Sainsbury Laboratory, Norwich, UK |
| contact email | jan.sklenar@tsl.ac.uk |
| dataset submitter |
Full Dataset Link List
| Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/03/PXD023178 |
| PRIDE project URI |
Repository Record List
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