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Granulocyte colony-stimulating factor for the treatment of biomaterial-associated staphylococcal infections in-vitro - Urolithiasis

  • ️Rohde, Detlef
  • ️Fri Dec 20 2002

Abstract

Staphylococcal infections are a common and severe complication after the implantation of a prosthesis. We developed an in-vitro model for biomaterial-associated infections and studied the effects of human recombinant granulocyte colony-stimulating factor (rhuG-CSF; filgrastime) on the eradication of bacteria from the surface of biomaterial. Latex beads (25 µm) were incubated with 107 colony forming units of either a slime producing (DSM 3269) or non-slime producing strain (ATCC 14990) of Staphylococcus epidermidis. Infected particles were consecutively confronted with effector cells, derived from heparinized whole blood samples taken from healthy volunteers, after stimulation with rhuG-CSF (5,000 IU/ml, 10,000 IU/ml). Control blood specimens were not stimulated or conditioned with normal saline. The results indicate that stimulation with rhuG-CSF induced an increased rate of phagocytosis and lead to a more rapid reduction of adhering bacteria from the surface of the beads. Therefore, the in-vitro data suggest that patients with prosthesis infection may profit from an additional treatment with rhuG-CSF.

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Acknowledgement

The rhuG-CSF (Neupogen) was kindly provided by Amgen Ltd., Munich, Germany.

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Authors and Affiliations

  1. Department of Urology, Universitiy of Aachen, Pauwelsstraße 30, 52057, Aachen, Germany

    Andrea Schlöbe & Detlef Rohde

  2. Institute of Microbiology and Immunology, Universitiy of Aachen, Pauwelsstraße 30, 52057, Aachen, Germany

    Norbert Schnitzler & Klaus Schweizer

Authors

  1. Andrea Schlöbe

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  2. Norbert Schnitzler

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  3. Klaus Schweizer

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  4. Detlef Rohde

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Correspondence to Detlef Rohde.

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Schlöbe, A., Schnitzler, N., Schweizer, K. et al. Granulocyte colony-stimulating factor for the treatment of biomaterial-associated staphylococcal infections in-vitro. Urol Res 30, 394–398 (2003). https://doi.org/10.1007/s00240-002-0289-7

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  • Received: 27 March 2002

  • Accepted: 06 November 2002

  • Published: 20 December 2002

  • Issue Date: February 2003

  • DOI: https://doi.org/10.1007/s00240-002-0289-7

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