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NT5E

5'-nucleotidase, ecto (CD73)

Identifiers

Symbols

NT5E; CD73; E5NT; NT; NT5; NTE; eN; eNT

External IDs

OMIM: 129190 MGI: 99782 HomoloGene: 1895 GeneCards: NT5E Gene

EC number

3.1.3.5

Gene Ontology
Molecular function	• nucleotide binding • ferrous iron binding • 5'-nucleotidase activity • hydrolase activity, acting on ester bonds • metal ion binding
Cellular component	• membrane fraction • cytoplasm • plasma membrane • plasma membrane • anchored to membrane
Biological process	• purine base metabolic process • purine nucleotide biosynthetic process • purine nucleotide catabolic process • AMP catabolic process • pyrimidine base metabolic process • DNA metabolic process • adenosine biosynthetic process • pyrimidine nucleoside catabolic process • negative regulation of inflammatory response • nucleobase, nucleoside and nucleotide metabolic process
Sources: Amigo / QuickGO

RNA expression pattern

More reference expression data

Orthologs

Species

Human

Mouse

RefSeq (mRNA)

RefSeq (protein)

Location (UCSC)

Chr 6:
86.16 – 86.21 Mb

Chr 9:
88.22 – 88.27 Mb

PubMed search

[1]

[2]

5'-nucleotidase (5'-NT), also known as ecto-5'-nucleotidase or CD73 (Cluster of Differentiation 73), is an enzyme that in humans is encoded by the NT5E gene.^[1]

Function

Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase; EC 3.1.3.5) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. Consequently, a deficiency of NT5 occurs in a variety of immunodeficiency diseases (e.g., see MIM 102700, MIM 300300). Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate.^[2]

References

^ Misumi Y, Ogata S, Ohkubo K, Hirose S, Ikehara Y (August 1990). "Primary structure of human placental 5'-nucleotidase and identification of the glycolipid anchor in the mature form". Eur. J. Biochem. 191 (3): 563–9. doi:10.1111/j.1432-1033.1990.tb19158.x. PMID 2129526.
^ "Entrez Gene: NT5E 5'-nucleotidase, ecto (CD73)". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=4907.

Resta R, Thompson LF (1997). "T cell signalling through CD73.". Cell. Signal. 9 (2): 131–9. doi:10.1016/S0898-6568(96)00132-5. PMID 9113412.
Kirchhoff C, Hale G (1997). "Cell-to-cell transfer of glycosylphosphatidylinositol-anchored membrane proteins during sperm maturation.". Mol. Hum. Reprod. 2 (3): 177–84. doi:10.1093/molehr/2.3.177. PMID 9238677.
Resta R, Yamashita Y, Thompson LF (1998). "Ecto-enzyme and signaling functions of lymphocyte CD73.". Immunol. Rev. 161: 95–109. doi:10.1111/j.1600-065X.1998.tb01574.x. PMID 9553767.
Rosi F, Carlucci F, Marinello E, Tabucchi A (2002). "Ecto-5'-nucleotidase in B-cell chronic lymphocytic leukemia.". Biomed. Pharmacother. 56 (2): 100–4. doi:10.1016/S0753-3322(01)00072-5. PMID 12000134.
Babiychuk EB, Draeger A (2006). "Regulation of ecto-5'-nucleotidase activity via Ca2+-dependent, annexin 2-mediated membrane rearrangement?". Biochem. Soc. Trans. 34 (Pt 3): 374–6. doi:10.1042/BST0340374. PMID 16709165.
Stefanovic V, Mandel P, Rosenberg A (1976). "Ecto-5'-nucleotidase of intact cultured C6 rat glioma cells.". J. Biol. Chem. 251 (13): 3900–5. PMID 819433.
Thomson LF, Ruedi JM, Glass A, et al. (1990). "Production and characterization of monoclonal antibodies to the glycosyl phosphatidylinositol-anchored lymphocyte differentiation antigen ecto-5'-nucleotidase (CD73).". Tissue Antigens 35 (1): 9–19. doi:10.1111/j.1399-0039.1990.tb01750.x. PMID 2137649.
Klemens MR, Sherman WR, Holmberg NJ, et al. (1990). "Characterization of soluble vs membrane-bound human placental 5'-nucleotidase.". Biochem. Biophys. Res. Commun. 172 (3): 1371–7. doi:10.1016/0006-291X(90)91601-N. PMID 2173922.
Boyle JM, Hey Y, Guerts van Kessel A, Fox M (1989). "Assignment of ecto-5'-nucleotidase to human chromosome 6.". Hum. Genet. 81 (1): 88–92. doi:10.1007/BF00283737. PMID 2848759.
Vlahović P, Stefanović V (1995). "Effect of dopamine on ecto-5'-nucleotidase expression in human glomerular mesangial cells.". Archives internationales de physiologie, de biochimie et de biophysique 102 (3): 171–3. PMID 8000038.
Hansen KR, Resta R, Webb CF, Thompson LF (1996). "Isolation and characterization of the promoter of the human 5'-nucleotidase (CD73)-encoding gene.". Gene 167 (1-2): 307–12. doi:10.1016/0378-1119(95)00574-9. PMID 8566797.
Airas L, Jalkanen S (1996). "CD73 mediates adhesion of B cells to follicular dendritic cells.". Blood 88 (5): 1755–64. PMID 8781432.
Airas L, Niemelä J, Salmi M, et al. (1997). "Differential regulation and function of CD73, a glycosyl-phosphatidylinositol-linked 70-kD adhesion molecule, on lymphocytes and endothelial cells.". J. Cell Biol. 136 (2): 421–31. doi:10.1083/jcb.136.2.421. PMC 2134816. PMID 9015312. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2134816.
Strohmeier GR, Lencer WI, Patapoff TW, et al. (1997). "Surface expression, polarization, and functional significance of CD73 in human intestinal epithelia.". J. Clin. Invest. 99 (11): 2588–601. doi:10.1172/JCI119447. PMC 508104. PMID 9169488. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=508104.
Aumüller G, Renneberg H, Schiemann PJ, et al. (1998). "The role of apocrine released proteins in the post-testicular regulation of human sperm function.". Adv. Exp. Med. Biol. 424: 193–219. PMID 9361795.
Rosi F, Agostinho AB, Carlucci F, et al. (1998). "Behaviour of human lymphocytic isoenzymes of 5'-nucleotidase.". Life Sci. 62 (25): 2257–66. doi:10.1016/S0024-3205(98)00206-9. PMID 9651114.

External links

MeSH NT5E+protein,+human

Categories:

Human proteins
Membrane protein stubs
Clusters of differentiation

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NT5E

Function

See also

References

Further reading

External links