Affinity chromatography, the Glossary

Table of Contents

1. 64 relations: Agarose, Alkaline phosphatase, Antibody, Antigen, Australian Veterinary Association, Avidin, Biomolecule, Biotin, Calmodulin, Chaperone (protein), Chemical compound, Chemoproteomics, Chromatography, Cobalt, Complementarity (molecular biology), Concanavalin A, Copper, Cysteine, Dye-ligand affinity chromatography, Elution, Enzyme, Functional group, Fusion protein, Glutathione, Glutathione S-transferase, Glycoprotein, His-tag, Histidine, Hormone, Hormone receptor, Imidazole, Iron, Kinase, Lectin, Ligand (biochemistry), Maltose, Meir Wilchek, Molecular binding, Molecular cloning, Monoclonal antibody, Nickel, Nitrilotriacetic acid, Nucleic acid, Pedro Cuatrecasas, Periodic counter-current chromatography, PH, Polyacrylamide, Polymer, Polysaccharide, Protease, ... Expand index (14 more) »

2. Biochemical separation processes

Agarose

Agarose is a heteropolysaccharide, generally extracted from certain red algae.

See Affinity chromatography and Agarose

Alkaline phosphatase

The enzyme alkaline phosphatase (ALP, alkaline phenyl phosphatase) is a phosphatase with the physiological role of dephosphorylating compounds.

See Affinity chromatography and Alkaline phosphatase

Antibody

An antibody (Ab) is the secreted form of a B cell receptor; the term immunoglobulin (Ig) can refer to either the membrane-bound form or the secreted form of the B cell receptor, but they are, broadly speaking, the same protein, and so the terms are often treated as synonymous.

See Affinity chromatography and Antibody

Antigen

In immunology, an antigen (Ag) is a molecule, moiety, foreign particulate matter, or an allergen, such as pollen, that can bind to a specific antibody or T-cell receptor.

See Affinity chromatography and Antigen

Australian Veterinary Association

The Australian Veterinary Association (AVA) is a not-for-profit association representing more than 7000 Australian veterinarians working in private practice, government, industry, and academia.

See Affinity chromatography and Australian Veterinary Association

Avidin

Avidin is a tetrameric biotin-binding protein produced in the oviducts of birds, reptiles and amphibians and deposited in the whites of their eggs.

See Affinity chromatography and Avidin

Biomolecule

A biomolecule or biological molecule is loosely defined as a molecule produced by a living organism and essential to one or more typically biological processes.

See Affinity chromatography and Biomolecule

Biotin

Biotin (also known as vitamin B7 or vitamin H) is one of the B vitamins.

See Affinity chromatography and Biotin

Calmodulin

Calmodulin (CaM) (an abbreviation for calcium-modulated protein) is a multifunctional intermediate calcium-binding messenger protein expressed in all eukaryotic cells.

See Affinity chromatography and Calmodulin

Chaperone (protein)

In molecular biology, molecular chaperones are proteins that assist the conformational folding or unfolding of large proteins or macromolecular protein complexes.

See Affinity chromatography and Chaperone (protein)

Chemical compound

A chemical compound is a chemical substance composed of many identical molecules (or molecular entities) containing atoms from more than one chemical element held together by chemical bonds.

See Affinity chromatography and Chemical compound

Chemoproteomics

Chemoproteomics (also known as chemical proteomics) entails a broad array of techniques used to identify and interrogate protein-small molecule interactions.

See Affinity chromatography and Chemoproteomics

Chromatography

In chemical analysis, chromatography is a laboratory technique for the separation of a mixture into its components.

See Affinity chromatography and Chromatography

Cobalt

Cobalt is a chemical element; it has symbol Co and atomic number 27.

See Affinity chromatography and Cobalt

Complementarity (molecular biology)

In molecular biology, complementarity describes a relationship between two structures each following the lock-and-key principle.

See Affinity chromatography and Complementarity (molecular biology)

Concanavalin A

Concanavalin A (ConA) is a lectin (carbohydrate-binding protein) originally extracted from the jack-bean (Canavalia ensiformis).

See Affinity chromatography and Concanavalin A

Copper

Copper is a chemical element; it has symbol Cu and atomic number 29.

See Affinity chromatography and Copper

Cysteine

Cysteine (symbol Cys or C) is a semiessential proteinogenic amino acid with the formula.

See Affinity chromatography and Cysteine

Dye-ligand affinity chromatography

Dye-ligand affinity chromatography is one of the Affinity chromatography techniques used for protein purification of a complex mixture. Affinity chromatography and Dye-ligand affinity chromatography are chromatography.

See Affinity chromatography and Dye-ligand affinity chromatography

Elution

In analytical and organic chemistry, elution is the process of extracting one material from another by washing with a solvent: washing of loaded ion-exchange resins to remove captured ions, or eluting proteins or other biopolymers from a gel electrophoresis or chromatography column. Affinity chromatography and elution are chromatography.

See Affinity chromatography and Elution

Enzyme

Enzymes are proteins that act as biological catalysts by accelerating chemical reactions.

See Affinity chromatography and Enzyme

Functional group

In organic chemistry, a functional group is a substituent or moiety in a molecule that causes the molecule's characteristic chemical reactions.

See Affinity chromatography and Functional group

Fusion protein

Fusion proteins or chimeric (kī-ˈmir-ik) proteins (literally, made of parts from different sources) are proteins created through the joining of two or more genes that originally coded for separate proteins.

See Affinity chromatography and Fusion protein

Glutathione

Glutathione (GSH) is an organic compound with the chemical formula.

See Affinity chromatography and Glutathione

Glutathione S-transferase

Glutathione S-transferases (GSTs), previously known as ligandins, are a family of eukaryotic and prokaryotic phase II metabolic isozymes best known for their ability to catalyze the conjugation of the reduced form of glutathione (GSH) to xenobiotic substrates for the purpose of detoxification.

See Affinity chromatography and Glutathione S-transferase

Glycoprotein

Glycoproteins are proteins which contain oligosaccharide (sugar) chains covalently attached to amino acid side-chains.

See Affinity chromatography and Glycoprotein

His-tag

A polyhistidine-tag, best known by the trademarked name His-tag, is an amino acid motif in proteins that typically consists of at least six histidine (His) residues, often at the N- or C-terminus of the protein. Affinity chromatography and his-tag are biochemical separation processes.

See Affinity chromatography and His-tag

Histidine

Histidine (symbol His or H) is an essential amino acid that is used in the biosynthesis of proteins.

See Affinity chromatography and Histidine

Hormone

A hormone (from the Greek participle ὁρμῶν, "setting in motion") is a class of signaling molecules in multicellular organisms that are sent to distant organs or tissues by complex biological processes to regulate physiology and behavior.

See Affinity chromatography and Hormone

Hormone receptor

A hormone receptor is a receptor molecule that binds to a specific hormone.

See Affinity chromatography and Hormone receptor

Imidazole

Imidazole (ImH) is an organic compound with the formula C3N2H4.

See Affinity chromatography and Imidazole

Iron

Iron is a chemical element.

See Affinity chromatography and Iron

Kinase

In biochemistry, a kinase is an enzyme that catalyzes the transfer of phosphate groups from high-energy, phosphate-donating molecules to specific substrates.

See Affinity chromatography and Kinase

Lectin

Lectins are carbohydrate-binding proteins that are highly specific for sugar groups that are part of other molecules, so cause agglutination of particular cells or precipitation of glycoconjugates and polysaccharides.

See Affinity chromatography and Lectin

Ligand (biochemistry)

In biochemistry and pharmacology, a ligand is a substance that forms a complex with a biomolecule to serve a biological purpose.

See Affinity chromatography and Ligand (biochemistry)

Maltose

Maltose, also known as maltobiose or malt sugar, is a disaccharide formed from two units of glucose joined with an α(1→4) bond. In the isomer isomaltose, the two glucose molecules are joined with an α(1→6) bond. Maltose is the two-unit member of the amylose homologous series, the key structural motif of starch.

See Affinity chromatography and Maltose

Meir Wilchek

Meir Wilchek (Hebrew: מאיר אשר וילצ'ק, born 17 October 1935) is an Israeli biochemist.

See Affinity chromatography and Meir Wilchek

Molecular binding

Molecular binding is an attractive interaction between two molecules that results in a stable association in which the molecules are in close proximity to each other.

See Affinity chromatography and Molecular binding

Molecular cloning

Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication within host organisms.

See Affinity chromatography and Molecular cloning

Monoclonal antibody

A monoclonal antibody (mAb, more rarely called moAb) is an antibody produced from a cell lineage made by cloning a unique white blood cell.

See Affinity chromatography and Monoclonal antibody

Nickel

Nickel is a chemical element; it has symbol Ni and atomic number 28.

See Affinity chromatography and Nickel

Nitrilotriacetic acid

Nitrilotriacetic acid (NTA) is the aminopolycarboxylic acid with the formula N(CH2CO2H)3.

See Affinity chromatography and Nitrilotriacetic acid

Nucleic acid

Nucleic acids are large biomolecules that are crucial in all cells and viruses.

See Affinity chromatography and Nucleic acid

Pedro Cuatrecasas

Pedro Cuatrecasas (born 27 September 1936) is an American biochemist and an Adjunct Professor of Pharmacology & Medicine at the University of California San Diego.

See Affinity chromatography and Pedro Cuatrecasas

Periodic counter-current chromatography

Periodic counter-current chromatography (PCC) is a method for running affinity chromatography in a quasi-continuous manner. Affinity chromatography and Periodic counter-current chromatography are chromatography.

See Affinity chromatography and Periodic counter-current chromatography

PH

In chemistry, pH, also referred to as acidity or basicity, historically denotes "potential of hydrogen" (or "power of hydrogen").

See Affinity chromatography and PH

Polyacrylamide

Polyacrylamide (abbreviated as PAM or pAAM) is a polymer with the formula (-CH2CHCONH2-). It has a linear-chain structure.

See Affinity chromatography and Polyacrylamide

Polymer

A polymer is a substance or material consisting of very large molecules linked together into chains of repeating subunits.

See Affinity chromatography and Polymer

Polysaccharide

Polysaccharides, or polycarbohydrates, are the most abundant carbohydrates found in food.

See Affinity chromatography and Polysaccharide

Protease

A protease (also called a peptidase, proteinase, or proteolytic enzyme) is an enzyme that catalyzes proteolysis, breaking down proteins into smaller polypeptides or single amino acids, and spurring the formation of new protein products.

See Affinity chromatography and Protease

Protein

Proteins are large biomolecules and macromolecules that comprise one or more long chains of amino acid residues.

See Affinity chromatography and Protein

Protein A

Protein A is a 42 kDa surface protein originally found in the cell wall of the bacteria Staphylococcus aureus.

See Affinity chromatography and Protein A

Protein G

Protein G is an immunoglobulin-binding protein expressed in group C and G streptococcal bacteria much like protein A but with differing binding specificities.

See Affinity chromatography and Protein G

Protein isoform

A protein isoform, or "protein variant", is a member of a set of highly similar proteins that originate from a single gene or gene family and are the result of genetic differences.

See Affinity chromatography and Protein isoform

Protein tag

Protein tags are peptide sequences genetically grafted onto a recombinant protein. Affinity chromatography and protein tag are biochemical separation processes.

See Affinity chromatography and Protein tag

Protein–protein interaction

Protein–protein interactions (PPIs) are physical contacts of high specificity established between two or more protein molecules as a result of biochemical events steered by interactions that include electrostatic forces, hydrogen bonding and the hydrophobic effect.

See Affinity chromatography and Protein–protein interaction

Receptor (biochemistry)

In biochemistry and pharmacology, receptors are chemical structures, composed of protein, that receive and transduce signals that may be integrated into biological systems.

See Affinity chromatography and Receptor (biochemistry)

Resolution (chromatography)

In chromatography, resolution is a measure of the separation of two peaks of different retention time t in a chromatogram. Affinity chromatography and resolution (chromatography) are chromatography.

See Affinity chromatography and Resolution (chromatography)

Substrate (chemistry)

In chemistry, the term substrate is highly context-dependent.

See Affinity chromatography and Substrate (chemistry)

Substrate analog

Substrate analogs (substrate state analogues), are chemical compounds with a chemical structure that resemble the substrate molecule in an enzyme-catalyzed chemical reaction.

See Affinity chromatography and Substrate analog

Thiocyanate

Thiocyanates are salts containing the thiocyanate anion (also known as rhodanide or rhodanate).

See Affinity chromatography and Thiocyanate

Thiol

In organic chemistry, a thiol, or thiol derivative, is any organosulfur compound of the form, where R represents an alkyl or other organic substituent.

See Affinity chromatography and Thiol

Tris

Tris, or tris(hydroxymethyl)aminomethane, or known during medical use as tromethamine or THAM, is an organic compound with the formula (HOCH2)3CNH2.

See Affinity chromatography and Tris

Zinc

Zinc is a chemical element with the symbol Zn and atomic number 30.

See Affinity chromatography and Zinc

See also

Biochemical separation processes

• Affinity chromatography
• Aldehyde tag
• Ammonium sulfate precipitation
• Cell fractionation
• Centrifuge
• Chromatin immunoprecipitation
• Chromosome conformation capture
• Cohn process
• Cross-flow filtration
• DNA extraction
• Dilution cloning
• Electrophoresis
• FLAG-tag
• Gel permeation chromatography
• HA-tag
• His-tag
• Immunoprecipitation
• Kidney dialysis
• Microfiltration
• Protein purification
• Protein tag
• RNA extraction
• SBP-tag
• Tandem affinity purification

References

[1] https://en.wikipedia.org/wiki/Affinity_chromatography

Also known as Affinity purification, Affinity selection, Boronate affinity chromatography, Chromatography, affinity, Immobilized metal affinity chromatography, Immobilized metal ion affinity chromatography, Immunoaffinity chromatography, Immunochromatographic, Immunochromatography, Lectin affinity chromatography, WAC technology, Weak Affinity Chromatography.

, Protein, Protein A, Protein G, Protein isoform, Protein tag, Protein–protein interaction, Receptor (biochemistry), Resolution (chromatography), Substrate (chemistry), Substrate analog, Thiocyanate, Thiol, Tris, Zinc.