en.unionpedia.org

Ammonium sulfate precipitation, the Glossary

Index Ammonium sulfate precipitation

Ammonium sulfate precipitation is one of the most commonly used methods for large and laboratory scale protein purification and fractionation that can be used to separate proteins by altering their solubility in the presence of a high salt concentration.[1]

Table of Contents

  1. 9 relations: Ammonium sulfate, Centrifugation, Hofmeister series, Ion chromatography, Salting in, Salting out, Size-exclusion chromatography, Solubility, Solvent effects.

  2. Biochemical separation processes

Ammonium sulfate

Ammonium sulfate (American English and international scientific usage; ammonium sulphate in British English); (NH4)2SO4, is an inorganic salt with a number of commercial uses.

See Ammonium sulfate precipitation and Ammonium sulfate

Centrifugation

Centrifugation is a mechanical process which involves the use of the centrifugal force to separate particles from a solution according to their size, shape, density, medium viscosity and rotor speed.

See Ammonium sulfate precipitation and Centrifugation

Hofmeister series

The Hofmeister series or lyotropic series is a classification of ions in order of their lyotrophic properties, which is the ability to salt out or salt in proteins.

See Ammonium sulfate precipitation and Hofmeister series

Ion chromatography

Ion chromatography (or ion-exchange chromatography) is a form of chromatography that separates ions and ionizable polar molecules based on their affinity to the ion exchanger.

See Ammonium sulfate precipitation and Ion chromatography

Salting in

Salting in refers to the effect where increasing the ionic strength of a solution increases the solubility of a solute, such as a protein.

See Ammonium sulfate precipitation and Salting in

Salting out

Salting out (also known as salt-induced precipitation, salt fractionation, anti-solvent crystallization, precipitation crystallization, or drowning out) is a purification technique that utilizes the reduced solubility of certain molecules in a solution of very high ionic strength.

See Ammonium sulfate precipitation and Salting out

Size-exclusion chromatography

Size-exclusion chromatography, also known as molecular sieve chromatography, is a chromatographic method in which molecules in solution are separated by their size, and in some cases molecular weight.

See Ammonium sulfate precipitation and Size-exclusion chromatography

Solubility

In chemistry, solubility is the ability of a substance, the solute, to form a solution with another substance, the solvent.

See Ammonium sulfate precipitation and Solubility

Solvent effects

In chemistry, solvent effects are the influence of a solvent on chemical reactivity or molecular associations.

See Ammonium sulfate precipitation and Solvent effects

See also

Biochemical separation processes

References

[1] https://en.wikipedia.org/wiki/Ammonium_sulfate_precipitation