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Cell fractionation, the Glossary

In cell biology, cell fractionation is the process used to separate cellular components while preserving individual functions of each component.^[1]

21 relations: Biochemistry, Buffer solution, Cell (biology), Cell biology, Cell disruption, Density, Differential centrifugation, Ficoll, Gauze, Homogenization (biology), Middle lamella, Organelle, Osmotic shock, Pectinase, Percoll, Protein, Suction filtration, Suspension culture, Tissue (biology), Tonicity, Ultrasound.
Biochemical separation processes
Fractionation

Biochemistry

Biochemistry or biological chemistry is the study of chemical processes within and relating to living organisms.

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Buffer solution

A buffer solution is a solution where the pH does not change significantly on dilution or if an acid or base is added at constant temperature.

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Cell (biology)

The cell is the basic structural and functional unit of all forms of life.

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Cell biology

Cell biology (also cellular biology or cytology) is a branch of biology that studies the structure, function, and behavior of cells.

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Cell disruption

Cell disruption is a method or process for releasing biological molecules from inside a cell.

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Density

Density (volumetric mass density or specific mass) is a substance's mass per unit of volume.

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In biochemistry and cell biology, differential centrifugation (also known as differential velocity centrifugation) is a common procedure used to separate organelles and other sub-cellular particles based on their sedimentation rate. Cell fractionation and differential centrifugation are Fractionation and Laboratory techniques.

See Cell fractionation and Differential centrifugation

Ficoll

Ficoll is a neutral, highly branched, high-mass, hydrophilic polysaccharide which dissolves readily in aqueous solutions.

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Gauze

Gauze is a thin, translucent fabric with a loose open weave.

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Homogenization (biology)

Homogenization, in cell biology or molecular biology, is a process whereby different fractions of a biological sample become equal in composition. Cell fractionation and Homogenization (biology) are Laboratory techniques.

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Middle lamella

The middle lamella is a layer that cements together the primary cell walls of two adjoining plant cells.

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Organelle

In cell biology, an organelle is a specialized subunit, usually within a cell, that has a specific function.

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Osmotic shock

Osmotic shock or osmotic stress is physiologic dysfunction caused by a sudden change in the solute concentration around a cell, which causes a rapid change in the movement of water across its cell membrane.

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Pectinase

Pectinases are a group of enzymes that breaks down pectin, a polysaccharide found in plant cell walls, through hydrolysis, transelimination and deesterification reactions.

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Percoll

Percoll is a tool for efficient density separation in Cell biology that was first formulated by Pertoft and colleagues.

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Protein

Proteins are large biomolecules and macromolecules that comprise one or more long chains of amino acid residues.

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Suction filtration

Vacuum filtration is a fast filtration technique used to separate solids from liquids. Cell fractionation and Suction filtration are Laboratory techniques.

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Suspension culture

A cell suspension or suspension culture is a type of cell culture in which single cells or small aggregates of cells are allowed to function and multiply in an agitated growth medium, thus forming a suspension.

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Tissue (biology)

In biology, tissue is an assembly of similar cells and their extracellular matrix from the same embryonic origin that together carry out a specific function.

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Tonicity

In chemical biology, tonicity is a measure of the effective osmotic pressure gradient; the water potential of two solutions separated by a partially-permeable cell membrane.

See Cell fractionation and Tonicity

Ultrasound

Ultrasound is sound with frequencies greater than 20 kilohertz.

See Cell fractionation and Ultrasound

References

[1] https://en.wikipedia.org/wiki/Cell_fractionation

Also known as Cellular fractionation, Sub-cellular fractionation, Subcellular fractionation, Subcellular fractions.